ZER1

Summary

ZER1 (zyg-11 related cell cycle regulator, HGNC:30960) is a protein-coding gene on chromosome 9q34.11, encoding Protein zer-1 homolog (Q7Z7L7). Serves as substrate adapter subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC.

This gene encodes a subunit of an E3 ubiquitin ligase complex that may be involved in meiosis. The encoded protein contains three leucine-rich repeat motifs.

Source: NCBI Gene 10444 — RefSeq curated summary.

At a glance

• GWAS associations: 11
• Clinical variants (ClinVar): 94 total
• Druggable target: yes
• MANE Select transcript: NM_006336

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30960
Approved symbol	ZER1
Name	zyg-11 related cell cycle regulator
Location	9q34.11
Locus type	gene with protein product
Status	Approved
Aliases	ZYG, Hzyg
Ensembl gene	ENSG00000160445
Ensembl biotype	protein_coding
OMIM	617764
Entrez	10444

Gene structure

Transcript identifiers

Ensembl transcripts: 34 — 33 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000291900, ENST00000414921, ENST00000427848, ENST00000494461, ENST00000873658, ENST00000873659, ENST00000929001, ENST00000929002, ENST00000929003, ENST00000929004, ENST00000929005, ENST00000929006, ENST00000929009, ENST00000929013, ENST00000929014, ENST00000960716, ENST00000960717, ENST00000960718, ENST00000960719, ENST00000960720, ENST00000960721, ENST00000960722, ENST00000960723, ENST00000960724, ENST00000960725, ENST00000960726, ENST00000960727, ENST00000960728, ENST00000960729, ENST00000960730, ENST00000960731, ENST00000960732, ENST00000960733, ENST00000960734

RefSeq mRNA: 7 — MANE Select: NM_006336 NM_001375954, NM_001375955, NM_001375956, NM_001375957, NM_001375958, NM_001375959, NM_006336

CCDS: CCDS6910

Canonical transcript exons

ENST00000291900 — 16 exons

Exon	Start	End
ENSE00001052402	128751413	128751527
ENSE00001052403	128751122	128751268
ENSE00001052404	128750616	128750789
ENSE00001052405	128739931	128740119
ENSE00001052406	128742530	128742745
ENSE00001052408	128752673	128752849
ENSE00001052410	128735334	128735431
ENSE00001052412	128740772	128740887
ENSE00001052414	128753164	128753600
ENSE00001052416	128741535	128741654
ENSE00001052418	128733426	128733528
ENSE00001052419	128741800	128741841
ENSE00001179277	128729786	128731394
ENSE00001347654	128771581	128771927
ENSE00001373790	128755408	128755659
ENSE00003638075	128753809	128753959

Expression profiles

Bgee: expression breadth ubiquitous, 253 present calls, max score 95.21.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 26.0891 / max 385.9950, expressed in 1805 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
102706	21.3117	1801
102705	3.1694	1468
102704	1.0296	595
102703	0.5784	327

Top tissues by expression

280 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right frontal lobe	UBERON:0002810	95.21	gold quality
prefrontal cortex	UBERON:0000451	94.63	gold quality
Brodmann (1909) area 9	UBERON:0013540	94.26	gold quality
gastrocnemius	UBERON:0001388	93.75	gold quality
hindlimb stylopod muscle	UBERON:0004252	93.56	gold quality
cingulate cortex	UBERON:0003027	93.52	gold quality
nucleus accumbens	UBERON:0001882	93.43	gold quality
anterior cingulate cortex	UBERON:0009835	93.38	gold quality
amygdala	UBERON:0001876	93.33	gold quality
muscle of leg	UBERON:0001383	93.16	gold quality
apex of heart	UBERON:0002098	93.16	gold quality
lower esophagus muscularis layer	UBERON:0035833	92.50	gold quality
frontal cortex	UBERON:0001870	92.49	gold quality
lower esophagus	UBERON:0013473	92.48	gold quality
right hemisphere of cerebellum	UBERON:0014890	92.48	gold quality
neocortex	UBERON:0001950	92.34	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	92.33	gold quality
putamen	UBERON:0001874	92.30	gold quality
cerebellar hemisphere	UBERON:0002245	92.21	gold quality
cerebellar cortex	UBERON:0002129	92.15	gold quality
caudate nucleus	UBERON:0001873	91.95	gold quality
hypothalamus	UBERON:0001898	91.93	gold quality
telencephalon	UBERON:0001893	91.72	gold quality
mucosa of stomach	UBERON:0001199	91.69	gold quality
Brodmann (1909) area 46	UBERON:0006483	91.69	gold quality
cerebral cortex	UBERON:0000956	91.66	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	91.66	gold quality
temporal lobe	UBERON:0001871	91.51	gold quality
forebrain	UBERON:0001890	91.50	gold quality
muscle layer of sigmoid colon	UBERON:0035805	91.48	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	8.77
E-GEOD-100618	no	362.50
E-MTAB-9467	no	0.66

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

164 targeting ZER1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-4692	100.00	67.32	2066
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-4481	100.00	66.42	1669
HSA-MIR-4747-5P	100.00	67.90	2681
HSA-MIR-5196-5P	100.00	67.98	2761
HSA-MIR-6740-5P	100.00	65.64	932
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-29A-3P	100.00	73.11	1835
HSA-MIR-29B-3P	100.00	73.18	1833
HSA-MIR-29C-3P	100.00	73.15	1833
HSA-MIR-4514	99.99	67.10	1870
HSA-MIR-19A-3P	99.98	75.33	2762
HSA-MIR-19B-3P	99.98	75.44	2754
HSA-MIR-4745-5P	99.98	65.95	1028
HSA-MIR-507	99.97	70.11	1915
HSA-MIR-6793-5P	99.97	65.95	758
HSA-MIR-557	99.96	70.01	1640
HSA-MIR-651-3P	99.94	73.48	5177
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-3143	99.93	71.96	3104
HSA-MIR-6835-3P	99.93	70.49	2904
HSA-MIR-450B-5P	99.92	71.48	3175
HSA-MIR-497-5P	99.92	71.83	2674

Literature-anchored findings (GeneRIF, showing 1)

• Molecular basis for recognition of Gly/N-degrons by CRL2(ZYG11B) and CRL2(ZER1). (PMID:34214466)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	zer1	ENSDARG00000105123
mus_musculus	Zer1	ENSMUSG00000039686
rattus_norvegicus	Zer1	ENSRNOG00000025832
drosophila_melanogaster	CG12084	FBGN0043458
caenorhabditis_elegans		WBGENE00020762

Paralogs (2): ZYG11B (ENSG00000162378), ZYG11A (ENSG00000203995)

Protein

Protein identifiers

Protein zer-1 homolog — Q7Z7L7 (reviewed: Q7Z7L7)

Alternative names: Hzyg, Zyg-11 homolog B-like protein, Zyg11b-like protein

All UniProt accessions (3): Q7Z7L7, X6RC68, X6RD87

UniProt curated annotations — full annotation on UniProt →

Function. Serves as substrate adapter subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC. Acts to target substrates bearing N-terminal degrons for proteasomal degradation with the first four residues of substrates being the key recognition elements. Involved in the clearance of proteolytic fragments generated by caspase cleavage during apoptosis since N-terminal glycine degrons are strongly enriched at caspase cleavage sites. Also important in the quality control of protein N-myristoylation in which N-terminal glycine degrons are conditionally exposed after a failure of N-myristoylation.

Subunit / interactions. Interacts with the ELOC-ELOB/Elongin BC complex. Part of an E3 ubiquitin ligase complex including ZER1, CUL2 and Elongin BC.

Tissue specificity. Expressed in testis, spermatocytes and spermatids (at protein level). Expressed in spermatocytes, spermatids, prostate, skeletal muscle, ovary, small intestine, heart, brain and pancreas.

Similarity. Belongs to the zyg-11 family.

RefSeq proteins (7): NP_001362883, NP_001362884, NP_001362885, NP_001362886, NP_001362887, NP_001362888, NP_006327* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000225	Armadillo	Repeat
IPR011989	ARM-like	Homologous_superfamily
IPR016024	ARM-type_fold	Homologous_superfamily
IPR032675	LRR_dom_sf	Homologous_superfamily
IPR051341	Zyg-11_UBL_adapter	Family
IPR055142	ZER1-like_C	Domain
IPR056845	LRR_Zer-1	Domain

Pfam: PF22964, PF25013

UniProt features (39 total): helix 16, repeat 8, sequence conflict 4, mutagenesis site 3, turn 3, initiator methionine 1, chain 1, modified residue 1, sequence variant 1, strand 1

Structure

Experimental structures (PDB)

6 structures.

PDB	Method	Resolution (Å)
7XYT	X-RAY DIFFRACTION	1.5
7EP3	X-RAY DIFFRACTION	1.51
7XYS	X-RAY DIFFRACTION	1.7
7EP5	X-RAY DIFFRACTION	2.02
7EP4	X-RAY DIFFRACTION	2.07
7XYU	X-RAY DIFFRACTION	2.7

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q7Z7L7-F1	90.92	0.77

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 2

Mutagenesis-validated functional residues (3):

Position	Phenotype
9	abolishes interaction with eloc.
552	complete loss of n-degron binding.
597	complete loss of n-degron binding.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 179 (showing top): TGGTGCT_MIR29A_MIR29B_MIR29C, AGGAAGC_MIR5163P, GOBP_REGULATION_OF_PROTEASOMAL_UBIQUITIN_DEPENDENT_PROTEIN_CATABOLIC_PROCESS, GOBP_POSITIVE_REGULATION_OF_PROTEIN_CATABOLIC_PROCESS, GOBP_MACROMOLECULE_CATABOLIC_PROCESS, TATTATA_MIR374, CACCAGC_MIR138, WANG_ESOPHAGUS_CANCER_VS_NORMAL_DN, GOBP_REGULATION_OF_CATABOLIC_PROCESS, GOBP_POSITIVE_REGULATION_OF_CATABOLIC_PROCESS, SHEDDEN_LUNG_CANCER_GOOD_SURVIVAL_A4, HOSHIDA_LIVER_CANCER_LATE_RECURRENCE_DN, GOBP_REGULATION_OF_PROTEIN_CATABOLIC_PROCESS, HELLER_HDAC_TARGETS_SILENCED_BY_METHYLATION_UP, GOBP_POSITIVE_REGULATION_OF_PROTEOLYSIS

GO Biological Process (2): protein quality control for misfolded or incompletely synthesized proteins (GO:0006515), positive regulation of proteasomal ubiquitin-dependent protein catabolic process (GO:0032436)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (1): Cul2-RING ubiquitin ligase complex (GO:0031462)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein catabolic process	1
regulation of proteasomal ubiquitin-dependent protein catabolic process	1
proteasome-mediated ubiquitin-dependent protein catabolic process	1
positive regulation of proteasomal protein catabolic process	1
positive regulation of ubiquitin-dependent protein catabolic process	1
binding	1
cullin-RING ubiquitin ligase complex	1

Protein interactions and networks

STRING

906 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZER1	CUL2	Q13617	882
ZER1	ELOC	Q15369	613
ZER1	LRR1	Q96L50	606
ZER1	ZYG11B	Q9C0D3	552
ZER1	ELOB	Q15370	529
ZER1	UBR4	Q5T4S7	507
ZER1	RBX1	P62877	499
ZER1	PPTC7	Q8NI37	476
ZER1	SNX11	Q9Y5W9	474
ZER1	CFAP45	Q9UL16	444
ZER1	VHL	P40337	422
ZER1	TSPAN2	O60636	420
ZER1	ZBTB44	Q8NCP5	420
ZER1	FPGS	Q05932	418
ZER1	CUL5	Q93034	411

IntAct

113 interactions, top by confidence:

A	B	Type	Score
POLG2	POLG	psi-mi:“MI:0914”(association)	0.950
DNM2	DNM1	psi-mi:“MI:0914”(association)	0.740
TEPSIN	AP4M1	psi-mi:“MI:0914”(association)	0.700
CDCA5	RAD21	psi-mi:“MI:0914”(association)	0.640
AGRP	TK1	psi-mi:“MI:0914”(association)	0.640
VEGFD	ADAM9	psi-mi:“MI:0914”(association)	0.640
MGME1	POLG	psi-mi:“MI:0914”(association)	0.640
TAFA4	NRP1	psi-mi:“MI:0914”(association)	0.530
GAST	ZZEF1	psi-mi:“MI:0914”(association)	0.530
LYG2	COL4A2	psi-mi:“MI:0914”(association)	0.530
SPINK7	UBB	psi-mi:“MI:0914”(association)	0.530
WFDC9	CTSC	psi-mi:“MI:0914”(association)	0.530
AGA	HBB	psi-mi:“MI:0914”(association)	0.530
IFNA2	IFIT3	psi-mi:“MI:0914”(association)	0.530
STMN2	MTA2	psi-mi:“MI:0914”(association)	0.530
SLAIN1	HSPA9	psi-mi:“MI:0914”(association)	0.530
CLPSL1	TNFAIP1	psi-mi:“MI:0914”(association)	0.530
TAFA1	ZER1	psi-mi:“MI:0914”(association)	0.530
HSPB8	VWA8	psi-mi:“MI:0914”(association)	0.530
MPP2	ABLIM1	psi-mi:“MI:0914”(association)	0.530
LYG2	TRAF2	psi-mi:“MI:0914”(association)	0.530
TSSC4	SNRNP200	psi-mi:“MI:0914”(association)	0.530
PRRC2B	ZER1	psi-mi:“MI:0915”(physical association)	0.500
K6	ZER1	psi-mi:“MI:0915”(physical association)	0.400

BioGRID (124): ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS), ZER1 (Affinity Capture-MS)

ESM2 similar proteins: A0JM56, A0JPI9, A2BFL2, A5PK13, A6H639, J9SQF3, O13066, P19686, P33402, Q02108, Q0VAA2, Q14BP6, Q15111, Q15813, Q3USB7, Q498T9, Q4R642, Q4V8D9, Q4ZHS0, Q5DU41, Q5FVQ9, Q5RAG3, Q5RBD9, Q5RJH2, Q5ZIJ9, Q5ZIU8, Q62688, Q68F79, Q6DN12, Q6GQN5, Q6NU09, Q6P9F7, Q6WRX3, Q7Z7L7, Q80ZJ6, Q8BG40, Q8CDU4, Q8CIR4, Q8CIV8, Q8HXA6

Diamond homologs: A0JMZ3, A2BFL2, P21541, Q3UFS0, Q5RAG3, Q5TYQ1, Q6WRX3, Q7Z7L7, Q80ZJ6, Q9C0D3, Q2WF59, Q9W0E8

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 154 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Late endosomal microautophagy	5	16.5×	4e-03
Clathrin-mediated endocytosis	8	6.9×	5e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

94 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	69
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2740 predictions. Top by Δscore:

Variant	Effect	Δscore
9:128733420:TCTTA:T	donor_loss	1.0000
9:128733421:CTTA:C	donor_loss	1.0000
9:128733422:TTACC:T	donor_loss	1.0000
9:128733423:TACC:T	donor_loss	1.0000
9:128733424:A:AC	donor_gain	1.0000
9:128733424:A:AG	donor_loss	1.0000
9:128733425:C:CC	donor_gain	1.0000
9:128733529:C:CC	acceptor_gain	1.0000
9:128740000:T:TA	donor_gain	1.0000
9:128740115:GGTTG:G	acceptor_gain	1.0000
9:128740116:GTTG:G	acceptor_gain	1.0000
9:128740117:TTG:T	acceptor_gain	1.0000
9:128740117:TTGCT:T	acceptor_loss	1.0000
9:128740118:TG:T	acceptor_gain	1.0000
9:128740118:TGC:T	acceptor_loss	1.0000
9:128740119:GCTG:G	acceptor_loss	1.0000
9:128740120:C:CC	acceptor_gain	1.0000
9:128740120:CT:C	acceptor_loss	1.0000
9:128740121:T:A	acceptor_loss	1.0000
9:128740124:C:CT	acceptor_gain	1.0000
9:128740125:A:T	acceptor_gain	1.0000
9:128740769:AAC:A	donor_loss	1.0000
9:128740770:A:AC	donor_gain	1.0000
9:128740771:C:CC	donor_gain	1.0000
9:128740771:CC:C	donor_loss	1.0000
9:128740883:AATTC:A	acceptor_gain	1.0000
9:128740884:ATTC:A	acceptor_gain	1.0000
9:128740885:TTC:T	acceptor_gain	1.0000
9:128740886:TC:T	acceptor_gain	1.0000
9:128740887:CC:C	acceptor_gain	1.0000

AlphaMissense

5091 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
9:128733520:A:G	Y717H	1.000
9:128735348:A:G	L709P	1.000
9:128735350:G:C	N708K	1.000
9:128735350:G:T	N708K	1.000
9:128735357:A:G	L706P	1.000
9:128735360:G:T	A705D	1.000
9:128735364:A:G	W704R	1.000
9:128735364:A:T	W704R	1.000
9:128735370:C:G	A702P	1.000
9:128735371:C:A	W701C	1.000
9:128735371:C:G	W701C	1.000
9:128735372:C:G	W701S	1.000
9:128735373:A:G	W701R	1.000
9:128735373:A:T	W701R	1.000
9:128735408:A:G	L689P	1.000
9:128735417:A:T	I686N	1.000
9:128735420:G:T	P685Q	1.000
9:128735430:A:G	S682P	1.000
9:128735431:C:A	R681S	1.000
9:128735431:C:G	R681S	1.000
9:128739931:C:A	R681M	1.000
9:128739931:C:G	R681T	1.000
9:128739932:T:C	R681G	1.000
9:128739934:T:C	Y680C	1.000
9:128739935:A:C	Y680D	1.000
9:128739935:A:G	Y680H	1.000
9:128739935:A:T	Y680N	1.000
9:128739936:A:C	N679K	1.000
9:128739936:A:T	N679K	1.000
9:128739940:A:G	I678T	1.000

dbSNP variants (sampled 300 via entrez): RS1000050324 (9:128741201 CAG>C), RS1000082425 (9:128744346 C>G), RS1000089370 (9:128759794 CA>C,CAA), RS1000200221 (9:128766275 G>C), RS1000240439 (9:128729633 T>G), RS1000280449 (9:128757817 C>T), RS1000396086 (9:128757499 G>A,C,T), RS1000430111 (9:128766565 G>C), RS1000542959 (9:128744796 G>C), RS1000546060 (9:128759529 C>G), RS1000552324 (9:128749577 C>T), RS1000602970 (9:128749874 T>C), RS1000642845 (9:128772241 C>T), RS1000672064 (9:128750954 C>T), RS1000713220 (9:128748319 G>A)

Disease associations

OMIM: gene MIM:617764 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

11 associations (top):

Study	Trait	p-value
GCST001959_7	Eating disorders (purging via substances)	5.000000e-06
GCST002115_14	Axial length	6.000000e-06
GCST005951_65	Body mass index	5.000000e-09
GCST008077_28	LDL cholesterol levels	5.000000e-06
GCST008077_82	LDL cholesterol levels	1.000000e-06
GCST008078_137	LDL cholesterol levels x alcohol consumption (regular vs non-regular drinkers) interaction (2df)	4.000000e-08
GCST008078_42	LDL cholesterol levels x alcohol consumption (regular vs non-regular drinkers) interaction (2df)	7.000000e-07
GCST008079_123	LDL cholesterol levels x alcohol consumption (drinkers vs non-drinkers) interaction (2df)	3.000000e-10
GCST008079_67	LDL cholesterol levels x alcohol consumption (drinkers vs non-drinkers) interaction (2df)	1.000000e-08
GCST008086_5	LDL cholesterol levels in current drinkers	7.000000e-07
GCST008086_82	LDL cholesterol levels in current drinkers	2.000000e-06

EFO canonical traits (4, from GWAS)

EFO ID	Trait name
EFO:0005318	axial length measurement
EFO:0004340	body mass index
EFO:0004611	low density lipoprotein cholesterol measurement
EFO:0004329	alcohol drinking

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL6066862 (PROTEIN-PROTEIN INTERACTION), CHEMBL6193837 (PROTEIN-PROTEIN INTERACTION)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

22 total (human), top 22 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases expression	2
Benzo(a)pyrene	affects methylation, increases expression	2
GSK-J4	increases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	increases expression	1
trichostatin A	affects expression	1
benzo(e)pyrene	decreases methylation	1
aflatoxin B2	decreases methylation	1
(+)-JQ1 compound	increases expression	1
Sunitinib	increases expression	1
Air Pollutants	increases abundance, increases expression	1
Dichlorodiphenyl Dichloroethylene	decreases expression	1
Ivermectin	decreases expression	1
Methapyrilene	decreases methylation	1
Methyl Methanesulfonate	increases expression	1
Selenium	increases expression	1
Smoke	decreases expression	1
Urethane	increases expression	1
Valproic Acid	increases methylation	1
Vitamin E	increases expression	1
Aflatoxin B1	decreases methylation	1
Particulate Matter	increases abundance, increases expression	1

ChEMBL screening assays

12 unique, capped per target: 12 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5554710	Binding	PROTAC (AATac) activity at ZYG11B/ZER1/EML4-ALK in human NCI-H3122 cells assessed as protein degradation upto 20 uM incubated for 72 hrs by immunoblot analysis	Distinct Amino Acid-Based PROTACs Target Oncogenic Kinases for Degradation in Non-Small Cell Lung Cancer (NSCLC). — J Med Chem

Cellosaurus cell lines

1 cell lines: 1 telomerase immortalized cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_C3LA	N/Tert-1 ZER1	Telomerase immortalized cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.