ZNF32

Summary

ZNF32 (zinc finger protein 32, HGNC:13095) is a protein-coding gene on chromosome 10q11.21, encoding Zinc finger protein 32 (P17041). May be involved in transcriptional regulation.

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in nucleus.

Source: NCBI Gene 7580 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 26 total
• MANE Select transcript: NM_006973

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:13095
Approved symbol	ZNF32
Name	zinc finger protein 32
Location	10q11.21
Locus type	gene with protein product
Status	Approved
Aliases	KOX30, ZNF637, Zfp637
Ensembl gene	ENSG00000169740
Ensembl biotype	protein_coding
OMIM	194539
Entrez	7580

Gene structure

Transcript identifiers

Ensembl transcripts: 15 — 14 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000374433, ENST00000395797, ENST00000485351, ENST00000858678, ENST00000858679, ENST00000858680, ENST00000858681, ENST00000858682, ENST00000858683, ENST00000924832, ENST00000924833, ENST00000924834, ENST00000924835, ENST00000924836, ENST00000924837

RefSeq mRNA: 7 — MANE Select: NM_006973 NM_001005368, NM_001324164, NM_001324165, NM_001324166, NM_001324167, NM_001324168, NM_006973

CCDS: CCDS7206

Canonical transcript exons

ENST00000374433 — 3 exons

Exon	Start	End
ENSE00001463505	43648802	43648881
ENSE00001930286	43643862	43644801
ENSE00003321447	43646064	43646202

Expression profiles

Bgee: expression breadth ubiquitous, 285 present calls, max score 95.50.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 7.9187 / max 88.6392, expressed in 1706 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
109187	5.2484	1631
109186	1.5649	962
109183	0.4633	235
109185	0.3497	184
109184	0.2924	150

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	95.50	gold quality
hindlimb stylopod muscle	UBERON:0004252	95.38	gold quality
ganglionic eminence	UBERON:0004023	95.19	gold quality
apex of heart	UBERON:0002098	95.16	gold quality
gastrocnemius	UBERON:0001388	95.05	gold quality
cortical plate	UBERON:0005343	95.02	gold quality
muscle of leg	UBERON:0001383	94.78	gold quality
nucleus accumbens	UBERON:0001882	94.67	gold quality
islet of Langerhans	UBERON:0000006	94.53	gold quality
putamen	UBERON:0001874	94.49	gold quality
right atrium auricular region	UBERON:0006631	94.37	gold quality
muscle organ	UBERON:0001630	94.32	gold quality
muscle layer of sigmoid colon	UBERON:0035805	94.32	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	94.31	gold quality
body of pancreas	UBERON:0001150	94.26	gold quality
amygdala	UBERON:0001876	94.22	gold quality
left ovary	UBERON:0002119	94.06	gold quality
cardiac atrium	UBERON:0002081	94.05	gold quality
caudate nucleus	UBERON:0001873	94.03	gold quality
body of uterus	UBERON:0009853	93.98	gold quality
lower esophagus muscularis layer	UBERON:0035833	93.97	gold quality
lower esophagus	UBERON:0013473	93.95	gold quality
heart left ventricle	UBERON:0002084	93.92	gold quality
cardiac ventricle	UBERON:0002082	93.88	gold quality
right frontal lobe	UBERON:0002810	93.82	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	93.70	gold quality
vastus lateralis	UBERON:0001379	93.66	gold quality
heart	UBERON:0000948	93.65	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	93.59	gold quality
right ovary	UBERON:0002118	93.56	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

4 targets.

Target	Regulation
CLN3
MYOD1	Repression
MYOG	Repression
TERT

miRNA regulators (miRDB)

20 targeting ZNF32, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-651-3P	99.94	73.48	5177
HSA-MIR-4760-3P	99.93	70.50	2385
HSA-MIR-335-3P	99.93	73.36	4958
HSA-MIR-4766-5P	99.75	69.23	2662
HSA-MIR-4729	99.69	72.18	4233
HSA-MIR-6751-5P	99.56	64.99	1145
HSA-MIR-1275	99.47	67.90	2749
HSA-MIR-942-5P	99.41	68.40	1977
HSA-MIR-6803-5P	99.19	63.90	1026
HSA-MIR-7109-5P	99.18	66.13	1057
HSA-MIR-625-5P	99.02	68.64	2031
HSA-MIR-4260	98.78	65.37	848
HSA-MIR-5572	98.55	65.84	970
HSA-MIR-6847-5P	97.93	66.74	1808
HSA-MIR-4665-5P	97.91	67.69	1536
HSA-MIR-450A-2-3P	97.91	67.56	1459
HSA-MIR-1298-5P	95.96	64.81	573
HSA-MIR-3157-3P	95.86	67.08	454
HSA-MIR-5191	95.22	64.69	354

Literature-anchored findings (GeneRIF, showing 7)

• ZNF32 acts as an effective autophagy inhibitor to protect breast cancer cells from excessive stimulus-autophagy-induced cell death. (PMID:25786368)
• Sp1-ZNF32-C1QBP axis protects against oxidative stress/apoptosis in hepatocellular carcinoma cells. (PMID:26497555)
• ZNF32 was found to directly bind to the TGF-betaR2 (transforming growth factor-beta receptor 2) promoter to promote its expression. (PMID:27763636)
• ZNF32 contributes to anoikis resistance by maintaining redox homeostasis and activating Src/FAK signaling in HepG2 cells. (PMID:30439540)
• We conclude that ZNF32 can engage GPER/ERK signalling and confer breast cancer stem cell-like properties, which may indicate poor prognosis of breast cancer patients. ZNF32 and GPER targeted therapies might provide new solutions for breast cancer treatment. (PMID:30478301)
• ZNF32 promotes the self-renewal of colorectal cancer cells by regulating the LEPR-STAT3 signaling pathway. (PMID:35115495)
• ZNF32 prevents the activation of cancer-associated fibroblasts through negative regulation of TGFB1 transcription in breast cancer. (PMID:36934389)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	si:zfos-932h1.3	ENSDARG00000098311
mus_musculus	Zfp637	ENSMUSG00000059689
rattus_norvegicus	Zfp637	ENSRNOG00000023065

Paralogs (176): ZNF195 (ENSG00000005801), ZNF112 (ENSG00000062370), ZNF275 (ENSG00000063587), ZNF37A (ENSG00000075407), ZNF510 (ENSG00000081386), ZNF506 (ENSG00000081665), ZNF268 (ENSG00000090612), MZF1 (ENSG00000099326), ZNF629 (ENSG00000102870), ZNF175 (ENSG00000105497), ZNF85 (ENSG00000105750), ZFP30 (ENSG00000120784), ZNF45 (ENSG00000124459), ZNF391 (ENSG00000124613), ZNF436 (ENSG00000125945), ZNF484 (ENSG00000127081), ZNF835 (ENSG00000127903), ZNF780B (ENSG00000128000), ZSCAN10 (ENSG00000130182), ZNF317 (ENSG00000130803), ZNF331 (ENSG00000130844), ZNF227 (ENSG00000131115), ZNF141 (ENSG00000131127), ZNF132 (ENSG00000131849), ZNF189 (ENSG00000136870), ZIM3 (ENSG00000141946), ZFP14 (ENSG00000142065), ZNF514 (ENSG00000144026), ZNF300 (ENSG00000145908), RBAK (ENSG00000146587), ZNF157 (ENSG00000147117), ZNF182 (ENSG00000147118), ZNF41 (ENSG00000147124), ZNF7 (ENSG00000147789), ZNF117 (ENSG00000152926), ZNF221 (ENSG00000159905), ZNF235 (ENSG00000159917), ZNF714 (ENSG00000160352), ZNF577 (ENSG00000161551), ZNF12 (ENSG00000164631)

Protein

Protein identifiers

Zinc finger protein 32 — P17041 (reviewed: P17041)

Alternative names: C2H2-546, Zinc finger protein KOX30

All UniProt accessions (1): P17041

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (7): NP_001005368, NP_001311093, NP_001311094, NP_001311095, NP_001311096, NP_001311097, NP_008904* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR013087	Znf_C2H2_type	Domain
IPR036236	Znf_C2H2_sf	Homologous_superfamily

Pfam: PF00096, PF13465

UniProt features (47 total): binding site 20, strand 8, zinc finger region 7, helix 7, turn 3, chain 1, sequence conflict 1

Structure

Experimental structures (PDB)

5 structures.

PDB	Method	Resolution (Å)
2EPC	SOLUTION NMR
2EPT	SOLUTION NMR
2EPU	SOLUTION NMR
2YTA	SOLUTION NMR
2YTB	SOLUTION NMR

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P17041-F1	79.04	0.68

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (20): 95; 99; 107; 110; 123; 127; 141; 144; 157; 161; 198; 201 …

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 116 (showing top): GSE18804_SPLEEN_MACROPHAGE_VS_TUMORAL_MACROPHAGE_DN, RRAGTTGT_UNKNOWN, GRUETZMANN_PANCREATIC_CANCER_DN, MORF_ATRX, BLALOCK_ALZHEIMERS_DISEASE_UP, TSENG_IRS1_TARGETS_DN, LEE_AGING_MUSCLE_DN, MORF_MT4, RIZKI_TUMOR_INVASIVENESS_3D_UP, CASTELLANO_NRAS_TARGETS_UP, GAZDA_DIAMOND_BLACKFAN_ANEMIA_ERYTHROID_DN, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, ER_Q6_01, KYNG_WERNER_SYNDROM_AND_NORMAL_AGING_DN, GOBERT_OLIGODENDROCYTE_DIFFERENTIATION_DN

GO Biological Process (1): regulation of transcription by RNA polymerase II (GO:0006357)

GO Molecular Function (7): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), sequence-specific double-stranded DNA binding (GO:1990837), DNA binding (GO:0003677), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
double-stranded DNA binding	1
sequence-specific DNA binding	1
nucleic acid binding	1
binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

306 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF32	FRA10AC1	Q70Z53	912
ZNF32	CRNKL1	Q9BZJ0	481
ZNF32	PLA2G5	P39877	432
ZNF32	ZNF414	Q96IQ9	404
ZNF32	ZFYVE19	Q96K21	395
ZNF32	SEC22A	Q96IW7	364
ZNF32	ZFAND2B	Q8WV99	344
ZNF32	TMEM101	Q96IK0	325
ZNF32	MED31	Q9Y3C7	322
ZNF32	TMEM54	Q969K7	321
ZNF32	NUP214	P35658	318
ZNF32	TMEM208	Q9BTX3	317
ZNF32	TMEM120A	Q9BXJ8	306
ZNF32	ZGPAT	Q8N5A5	305
ZNF32	ZMYM4	Q5VZL5	285

IntAct

38 interactions, top by confidence:

A	B	Type	Score
ZNF32	RELL2	psi-mi:“MI:0915”(physical association)	0.590
KRTAP10-5	ZNF32	psi-mi:“MI:0915”(physical association)	0.560
KRTAP10-4	ZNF32	psi-mi:“MI:0915”(physical association)	0.560
ZNF32	KRTAP10-7	psi-mi:“MI:0915”(physical association)	0.560
ZNF32	KRTAP10-8	psi-mi:“MI:0915”(physical association)	0.560
NOTCH2NLA	ZNF32	psi-mi:“MI:0915”(physical association)	0.560
	ZNF32	psi-mi:“MI:0915”(physical association)	0.560
ZNF32	KRTAP10-5	psi-mi:“MI:0915”(physical association)	0.560
KRTAP10-7	ZNF32	psi-mi:“MI:0915”(physical association)	0.560
KRTAP10-8	ZNF32	psi-mi:“MI:0915”(physical association)	0.560
ZNF32		psi-mi:“MI:0915”(physical association)	0.560
ZNF32	KRTAP10-4	psi-mi:“MI:0915”(physical association)	0.560
ZNF32	NOTCH2NLA	psi-mi:“MI:0915”(physical association)	0.560
ZNF32	CARD10	psi-mi:“MI:0915”(physical association)	0.560
GOLGA6L9	ZNF32	psi-mi:“MI:0915”(physical association)	0.560
ZNF32	CCDC125	psi-mi:“MI:0915”(physical association)	0.560
ZNF32	INCA1	psi-mi:“MI:0915”(physical association)	0.560
GRB2	ZNF32	psi-mi:“MI:0915”(physical association)	0.400
ZNF32	NRDE2	psi-mi:“MI:0915”(physical association)	0.400
ZNF32	ULK2	psi-mi:“MI:0915”(physical association)	0.370
POT1	ZNF32	psi-mi:“MI:0915”(physical association)	0.370
NOTCH2	ZNF320	psi-mi:“MI:0914”(association)	0.350
ZNF32	CARD10	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (16): KRTAP10-4 (Two-hybrid), KRTAP10-7 (Two-hybrid), KRTAP10-5 (Two-hybrid), KRTAP10-8 (Two-hybrid), KRTAP10-3 (Two-hybrid), NOTCH2NL (Two-hybrid), RELL2 (Affinity Capture-MS), ZNF32 (Two-hybrid), CCDC125 (Two-hybrid), INCA1 (Two-hybrid), GOLGA6L9 (Two-hybrid), ZNF32 (Affinity Capture-MS), RELL2 (Affinity Capture-MS), NRDE2 (Affinity Capture-MS), ZNF32 (Two-hybrid)

ESM2 similar proteins: A2A761, A4II20, B1H2Q6, G5EGQ2, O08954, O42409, O43623, O73691, O73692, O73693, P08046, P08154, P10925, P17023, P17031, P17041, P18146, P18723, P18725, P19382, P26632, P79958, P97469, Q10R93, Q14592, Q29RZ4, Q29W20, Q3MHQ4, Q3V080, Q5R7I8, Q5RBY9, Q61751, Q6DCW1, Q6GQH4, Q6NTY6, Q6PK81, Q6ZSS3, Q8BI99, Q8BIQ3, Q8N988

Diamond homologs: P17041, P18732, Q08AN1, Q3US17, Q80V23, Q86T29, Q8CF60, Q8TBZ5, Q96BV0, Q96EG3, Q9H4T2, Q9H7R5, Q9HAH1, O15090, O43167, P18744, P18747, Q5EBL2, Q62255, Q80X44, Q8CJ78, Q8K083, Q9BXA9, Q9ER74, Q9NSC2, Q9UDV7, A1YEV9, A1YG26, A1YG60, A2ANX9, A2T712, A2T736, A2T7L7, A3KN32, A7Y7X5, B0K011, E9Q6W4, O14771, O15391, O35738

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

26 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	26
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

740 predictions. Top by Δscore:

Variant	Effect	Δscore
10:43645979:T:TA	donor_gain	1.0000
10:43646054:A:AC	donor_gain	1.0000
10:43646055:C:CC	donor_gain	1.0000
10:43646055:CTCA:C	donor_gain	1.0000
10:43646058:A:AC	donor_gain	1.0000
10:43646059:C:CC	donor_gain	1.0000
10:43646059:CT:C	donor_gain	1.0000
10:43646059:CTCA:C	donor_gain	1.0000
10:43646062:A:AC	donor_gain	1.0000
10:43646063:C:CC	donor_gain	1.0000
10:43646076:CATT:C	donor_gain	1.0000
10:43646088:ATCTT:A	donor_gain	1.0000
10:43646089:T:C	donor_gain	1.0000
10:43646092:T:TA	donor_gain	1.0000
10:43644799:CAT:C	acceptor_gain	0.9900
10:43644802:C:CC	acceptor_gain	0.9900
10:43644802:CTG:C	acceptor_loss	0.9900
10:43644805:A:C	acceptor_gain	0.9900
10:43646058:ACT:A	donor_gain	0.9900
10:43646059:CTC:C	donor_gain	0.9900
10:43646063:CTG:C	donor_gain	0.9900
10:43646079:T:A	donor_gain	0.9900
10:43646093:C:A	donor_gain	0.9900
10:43646149:CGA:C	donor_gain	0.9900
10:43646200:CAC:C	acceptor_gain	0.9900
10:43648577:T:TA	donor_gain	0.9900
10:43648688:ATTT:A	donor_gain	0.9900
10:43648691:T:TA	donor_gain	0.9900
10:43644797:CACAT:C	acceptor_gain	0.9800
10:43646063:CTGA:C	donor_gain	0.9800

AlphaMissense

1808 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
10:43644362:G:C	F170L	0.999
10:43644362:G:T	F170L	0.999
10:43644364:A:G	F170L	0.999
10:43644446:G:C	F142L	0.999
10:43644446:G:T	F142L	0.999
10:43644448:A:G	F142L	0.999
10:43644498:C:G	R125P	0.999
10:43644530:G:C	F114L	0.999
10:43644530:G:T	F114L	0.999
10:43644532:A:G	F114L	0.999
10:43644278:G:C	F198L	0.998
10:43644278:G:T	F198L	0.998
10:43644280:A:G	F198L	0.998
10:43644503:A:C	H123Q	0.998
10:43644503:A:T	H123Q	0.998
10:43644513:A:G	L120P	0.998
10:43644614:G:C	F86L	0.998
10:43644614:G:T	F86L	0.998
10:43644616:A:G	F86L	0.998
10:43644194:G:C	F226L	0.997
10:43644194:G:T	F226L	0.997
10:43644196:A:G	F226L	0.997
10:43644345:A:G	L176P	0.997
10:43644447:A:G	F142S	0.997
10:43644491:G:C	H127Q	0.997
10:43644491:G:T	H127Q	0.997
10:43644501:T:G	Q124P	0.997
10:43644505:G:C	H123D	0.997
10:43644505:G:T	H123N	0.997
10:43644531:A:G	F114S	0.997

dbSNP variants (sampled 300 via entrez): RS1000943663 (10:43648362 G>A), RS1001261314 (10:43646949 T>C), RS1001313897 (10:43648560 A>C), RS1001861571 (10:43645139 A>G), RS1002336711 (10:43645505 G>A,T), RS1002364316 (10:43648264 G>A,C), RS1002572377 (10:43644790 C>T), RS1002645379 (10:43648568 C>T), RS1002717505 (10:43648831 C>A,T), RS1004334297 (10:43648864 G>C), RS1004455288 (10:43648839 A>C,G), RS1005035115 (10:43650537 A>C), RS1005071702 (10:43650230 C>T), RS1005707533 (10:43647978 G>A), RS1005752118 (10:43647765 G>A)

Disease associations

OMIM: gene MIM:194539 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST010479_14	Coronary artery disease	1.000000e-12

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

36 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
potassium chromate(VI)	decreases expression, affects cotreatment	2
dicrotophos	decreases expression	1
bisphenol A	affects cotreatment, increases expression	1
beta-methylcholine	affects expression	1
epigallocatechin gallate	affects cotreatment, decreases expression	1
di-n-butylphosphoric acid	affects expression	1
chromium hexavalent ion	decreases expression	1
abrine	decreases expression	1
jinfukang	affects cotreatment, increases expression	1
(+)-JQ1 compound	decreases expression	1
Irinotecan	affects cotreatment, increases response to substance	1
Resveratrol	affects cotreatment, increases expression	1
Temozolomide	increases expression	1
Sunitinib	increases expression	1
Leflunomide	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Atrazine	decreases expression	1
Benzo(a)pyrene	affects methylation	1
Cisplatin	affects cotreatment, increases expression	1
Leucovorin	affects cotreatment, increases response to substance	1
Dexamethasone	affects cotreatment, increases expression	1
Dimethyl Sulfoxide	increases expression	1
Doxorubicin	increases expression	1
Estradiol	decreases expression	1
Etoposide	affects response to substance	1
Fluorouracil	affects cotreatment, increases response to substance	1
Indomethacin	affects cotreatment, increases expression	1
Oxygen	decreases expression	1
Plant Extracts	affects cotreatment, increases expression	1
Zinc	decreases expression	1

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_HD19	HEK293 eGFP-ZNF32	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.