ZNF324B

Summary

ZNF324B (zinc finger protein 324B, HGNC:33107) is a protein-coding gene on chromosome 19q13.43, encoding Zinc finger protein 324B (Q6AW86). May be involved in transcriptional regulation.

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.

Source: NCBI Gene 388569 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 100 total
• MANE Select transcript: NM_207395

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33107
Approved symbol	ZNF324B
Name	zinc finger protein 324B
Location	19q13.43
Locus type	gene with protein product
Status	Approved
Aliases	FLJ45850
Ensembl gene	ENSG00000249471
Ensembl biotype	protein_coding
Entrez	388569

Gene structure

Transcript identifiers

Ensembl transcripts: 19 — 19 protein_coding

ENST00000336614, ENST00000545523, ENST00000594214, ENST00000598244, ENST00000599193, ENST00000599194, ENST00000869294, ENST00000869295, ENST00000869296, ENST00000869297, ENST00000869298, ENST00000928892, ENST00000928893, ENST00000963232, ENST00000963233, ENST00000963234, ENST00000963235, ENST00000963236, ENST00000963237

RefSeq mRNA: 1 — MANE Select: NM_207395 NM_207395

CCDS: CCDS33138

Canonical transcript exons

ENST00000336614 — 4 exons

Exon	Start	End
ENSE00001469474	58455183	58457833
ENSE00001469476	58451622	58451704
ENSE00003677496	58453696	58453822
ENSE00003789631	58454228	58454344

Expression profiles

Bgee: expression breadth ubiquitous, 163 present calls, max score 81.91.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 4.2841 / max 48.3036, expressed in 1620 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
177846	4.2841	1620

Top tissues by expression

275 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	81.91	silver quality
cortical plate	UBERON:0005343	76.71	gold quality
sural nerve	UBERON:0015488	76.30	gold quality
stromal cell of endometrium	CL:0002255	74.42	gold quality
ventricular zone	UBERON:0003053	73.07	gold quality
prefrontal cortex	UBERON:0000451	72.77	gold quality
pancreatic ductal cell	CL:0002079	72.43	silver quality
ganglionic eminence	UBERON:0004023	72.13	gold quality
gastrocnemius	UBERON:0001388	71.72	gold quality
granulocyte	CL:0000094	71.58	gold quality
muscle of leg	UBERON:0001383	71.12	gold quality
cervix squamous epithelium	UBERON:0006922	71.06	gold quality
right uterine tube	UBERON:0001302	70.72	gold quality
skin of leg	UBERON:0001511	70.68	gold quality
endocervix	UBERON:0000458	70.48	gold quality
islet of Langerhans	UBERON:0000006	70.42	gold quality
skin of abdomen	UBERON:0001416	70.16	gold quality
leukocyte	CL:0000738	70.15	gold quality
right ovary	UBERON:0002118	70.02	gold quality
monocyte	CL:0000576	70.01	gold quality
mononuclear cell	CL:0000842	69.83	gold quality
body of pancreas	UBERON:0001150	69.70	gold quality
lower esophagus muscularis layer	UBERON:0035833	69.67	gold quality
lower esophagus	UBERON:0013473	69.62	gold quality
ectocervix	UBERON:0012249	69.41	gold quality
pancreas	UBERON:0001264	69.30	gold quality
mucosa of stomach	UBERON:0001199	69.24	gold quality
esophagus	UBERON:0001043	69.22	gold quality
esophagus mucosa	UBERON:0002469	69.15	gold quality
body of stomach	UBERON:0001161	69.13	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.55

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

33 targeting ZNF324B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3689D	100.00	66.14	1181
HSA-MIR-6851-5P	100.00	65.63	1294
HSA-MIR-6798-5P	100.00	65.77	699
HSA-MIR-5193	100.00	67.26	1744
HSA-MIR-4534	99.99	66.58	1907
HSA-MIR-3667-3P	99.99	67.17	1636
HSA-MIR-3605-5P	99.96	67.12	932
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-4493	99.90	66.48	977
HSA-MIR-4447	99.85	67.81	2900
HSA-MIR-7110-5P	99.80	67.84	1712
HSA-MIR-6842-5P	99.80	67.54	1587
HSA-MIR-3202	99.66	67.70	2737
HSA-MIR-4753-5P	99.54	68.51	1356
HSA-MIR-136-5P	99.50	67.26	1153
HSA-MIR-2392	99.43	67.50	708
HSA-MIR-8077	99.17	66.67	862
HSA-MIR-4695-5P	99.06	64.87	1151
HSA-MIR-589-5P	98.72	66.96	927
HSA-MIR-1227-5P	98.65	65.32	1549
HSA-MIR-6852-3P	98.54	67.60	1468
HSA-MIR-7114-5P	98.51	67.87	1349
HSA-MIR-4684-3P	98.24	69.91	1075
HSA-MIR-4717-5P	98.19	67.97	894
HSA-MIR-4518	98.12	66.82	1030
HSA-MIR-432-5P	98.00	68.13	989
HSA-MIR-6502-3P	97.86	65.43	569
HSA-MIR-3664-3P	97.85	67.62	1452
HSA-MIR-1266-5P	97.71	66.92	1052
HSA-MIR-6859-3P	97.26	64.69	428

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	42sp43	ENSDARG00000104170
mus_musculus	Zfp324	ENSMUSG00000004500
rattus_norvegicus	Zfp324	ENSRNOG00000027436
drosophila_melanogaster	dwg	FBGN0000520
drosophila_melanogaster	CG6654	FBGN0038301
caenorhabditis_elegans		WBGENE00003933

Paralogs (6): ZNF324 (ENSG00000083812), ZBTB47 (ENSG00000114853), GTF3A (ENSG00000122034), ZUP1 (ENSG00000153975), ZNF513 (ENSG00000163795), ZNF652 (ENSG00000198740)

Protein

Protein identifiers

Zinc finger protein 324B — Q6AW86 (reviewed: Q6AW86)

All UniProt accessions (5): Q6AW86, M0R090, M0R0B1, M0R1X9, M0R3B5

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q6AW86-1	1	yes
Q6AW86-2	2

RefSeq proteins (1): NP_997278* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily

Pfam: PF00096, PF01352

UniProt features (19 total): zinc finger region 9, region of interest 2, sequence conflict 2, chain 1, domain 1, compositionally biased region 1, cross-link 1, splice variant 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q6AW86-F1	64.80	0.35

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 214

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 36 (showing top): PARENT_MTOR_SIGNALING_UP, MOREAUX_MULTIPLE_MYELOMA_BY_TACI_UP, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, SMAD_Q6, TORCHIA_TARGETS_OF_EWSR1_FLI1_FUSION_UP, ATF2_S_UP.V1_UP, ATF2_UP.V1_UP, IL21_UP.V1_DN, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, CEBPZ_TARGET_GENES, PCGF1_TARGET_GENES, ZNF669_TARGET_GENES, ZNF768_TARGET_GENES, MIR3202, GSE10240_IL17_VS_IL17_AND_IL22_STIM_PRIMARY_BRONCHIAL_EPITHELIAL_CELLS_UP

GO Biological Process (2): regulation of transcription by RNA polymerase II (GO:0006357), regulation of DNA-templated transcription (GO:0006355)

GO Molecular Function (5): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

574 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF324B	EID2	Q8N6I1	635
ZNF324B	RABEP2	Q9H5N1	633
ZNF324B	YIPF4	Q9BSR8	540
ZNF324B	FRMD5	Q7Z6J6	480
ZNF324B	CCDC127	Q96BQ5	445
ZNF324B	ASB7	Q9H672	414
ZNF324B	SUPT7L	O94864	409
ZNF324B	CAPN10	Q9HC96	403
ZNF324B	TMC8	Q8IU68	400
ZNF324B	PCMT1	P22061	391
ZNF324B	RPS7	P23821	388
ZNF324B	CNOT10	Q9H9A5	362
ZNF324B	COMMD5	Q9GZQ3	343
ZNF324B	TADA2B	Q86TJ2	329
ZNF324B	GJD3	Q8N144	329

IntAct

12 interactions, top by confidence:

A	B	Type	Score
ZNF324B	ZNF316	psi-mi:“MI:0914”(association)	0.530
ZNF324B	ZNF324	psi-mi:“MI:0914”(association)	0.530
KISS1R	ERLIN1	psi-mi:“MI:0915”(physical association)	0.400
RGS3	ZNF646	psi-mi:“MI:0914”(association)	0.350
CBX5	ZNF568	psi-mi:“MI:0914”(association)	0.350
NOTCH2	ZNF320	psi-mi:“MI:0914”(association)	0.350
LTBP2	ZNF320	psi-mi:“MI:0914”(association)	0.350
LRP3	TMEM131L	psi-mi:“MI:0914”(association)	0.350

BioGRID (150): ZNF324 (Affinity Capture-MS), SPIN3 (Affinity Capture-MS), ACTA1 (Affinity Capture-MS), ZNF324B (Affinity Capture-MS), RBAK (Affinity Capture-MS), TRIM24 (Affinity Capture-MS), SPIN1 (Affinity Capture-MS), NGRN (Affinity Capture-MS), HELZ2 (Affinity Capture-MS), ZNF184 (Affinity Capture-MS), ZNF460 (Affinity Capture-MS), ZNF746 (Affinity Capture-MS), ZNF316 (Affinity Capture-MS), TOPORS (Affinity Capture-MS), RPF1 (Affinity Capture-MS)

ESM2 similar proteins: A3KN32, A6NM28, A8K8V0, E9PYI1, O75467, O95201, P10072, P10075, P10078, P10754, P28698, P30373, P52736, Q0VCC5, Q14929, Q16587, Q17QR7, Q29RZ4, Q3KNW1, Q3TDE8, Q3US17, Q58DK7, Q5RJR4, Q5T619, Q5TEC3, Q5U4E2, Q62396, Q68H95, Q6AW86, Q6PGE4, Q6ZMY9, Q7Z398, Q7Z7K2, Q8BI73, Q8BIF9, Q8BIQ3, Q8BKK5, Q8JZL0, Q8N393, Q8R0T2

Diamond homologs: A0JPL0, A2A761, A3KN32, A3KN36, A6NFI3, A6NM28, A6QLU5, A6QPT6, A7MBI1, A8MTY0, B2RXC5, B4DU55, E9PYI1, O14978, O60765, O75467, O75820, O95780, P0C7X2, P0DKX0, P0DPD5, P10072, P15622, P16374, P17014, P17023, P17032, P17098, P51523, P51786, P52738, P52742, Q02975, Q06732, Q08DG8, Q08ER8, Q0VCB0, Q14590, Q14929, Q29RZ4

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

100 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	87
Likely benign	12
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1216 predictions. Top by Δscore:

Variant	Effect	Δscore
19:58453685:T:TA	acceptor_gain	1.0000
19:58453688:T:A	acceptor_gain	1.0000
19:58453691:TTTAG:T	acceptor_loss	1.0000
19:58453694:A:AG	acceptor_gain	1.0000
19:58453694:AG:A	acceptor_gain	1.0000
19:58453695:G:GA	acceptor_gain	1.0000
19:58453695:GG:G	acceptor_gain	1.0000
19:58453695:GGA:G	acceptor_gain	1.0000
19:58453695:GGAC:G	acceptor_gain	1.0000
19:58453695:GGACC:G	acceptor_gain	1.0000
19:58453818:ACTTG:A	donor_gain	1.0000
19:58453819:CTTG:C	donor_gain	1.0000
19:58453823:G:GG	donor_gain	1.0000
19:58453824:T:G	donor_loss	1.0000
19:58453828:GCCC:G	donor_gain	1.0000
19:58454223:CACA:C	acceptor_loss	1.0000
19:58454225:CAGG:C	acceptor_loss	1.0000
19:58454226:A:AG	acceptor_gain	1.0000
19:58454226:A:T	acceptor_loss	1.0000
19:58454227:G:GG	acceptor_gain	1.0000
19:58454227:GGA:G	acceptor_gain	1.0000
19:58454227:GGACT:G	acceptor_gain	1.0000
19:58454252:T:TA	acceptor_gain	1.0000
19:58454345:G:GG	donor_gain	1.0000
19:58454345:G:T	donor_loss	1.0000
19:58451655:G:GA	donor_gain	0.9900
19:58451702:A:T	donor_gain	0.9900
19:58451754:G:GG	donor_gain	0.9900
19:58451828:G:GT	donor_gain	0.9900
19:58453820:TTG:T	donor_gain	0.9900

AlphaMissense

3552 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:58455824:T:C	F294L	0.999
19:58455826:C:A	F294L	0.999
19:58455826:C:G	F294L	0.999
19:58455908:T:C	F322L	0.999
19:58455910:C:A	F322L	0.999
19:58455910:C:G	F322L	0.999
19:58455992:T:C	F350L	0.999
19:58455994:C:A	F350L	0.999
19:58455994:C:G	F350L	0.999
19:58456076:T:C	F378L	0.999
19:58456078:C:A	F378L	0.999
19:58456078:C:G	F378L	0.999
19:58455740:T:C	F266L	0.998
19:58455742:C:A	F266L	0.998
19:58455742:C:G	F266L	0.998
19:58455759:T:C	L272P	0.998
19:58455769:C:A	H275Q	0.998
19:58455769:C:G	H275Q	0.998
19:58456160:T:C	F406L	0.998
19:58456162:C:A	F406L	0.998
19:58456162:C:G	F406L	0.998
19:58456244:T:C	F434L	0.998
19:58456246:T:A	F434L	0.998
19:58456246:T:G	F434L	0.998
19:58456328:T:C	F462L	0.998
19:58456330:C:A	F462L	0.998
19:58456330:C:G	F462L	0.998
19:58456412:T:C	F490L	0.998
19:58456414:C:A	F490L	0.998
19:58456414:C:G	F490L	0.998

dbSNP variants (sampled 300 via entrez): RS1000128585 (19:58418548 C>T), RS1000253189 (19:58420376 A>G), RS1000304254 (19:58435991 T>G), RS1000334350 (19:58419284 G>A,T), RS1000420382 (19:58452022 G>T), RS1000528968 (19:58458115 G>A), RS1000635398 (19:58447892 G>A), RS1000696123 (19:58446127 T>C,G), RS1000726993 (19:58446420 A>G), RS1000786057 (19:58438288 C>T), RS1000796266 (19:58442421 G>A,C,T), RS1000863909 (19:58441361 T>G), RS1000891652 (19:58429962 C>A,T), RS1000960032 (19:58425136 G>C), RS1000975710 (19:58425561 G>C)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST004599_201	Mean platelet volume	1.000000e-10

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

12 total (human), top 12 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
GSK-J4	decreases expression	1
triphenyl phosphate	affects expression	1
ferrous chloride	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
Arsenic	affects methylation	1
Benzo(a)pyrene	decreases methylation	1
Calcitriol	decreases expression, affects cotreatment	1
Estradiol	increases expression	1
Smoke	decreases expression	1
Testosterone	affects cotreatment, decreases expression	1
Urethane	decreases expression	1
Valproic Acid	increases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.