ZNF33B

Summary

ZNF33B (zinc finger protein 33B, HGNC:13097) is a protein-coding gene on chromosome 10q11.21, encoding Zinc finger protein 33B (Q06732). May be involved in transcriptional regulation.

This gene encodes a member of the zinc finger family of proteins. This gene shows decreased expression in cumulus cells derived from patients undergoing controlled ovarian stimulation. This gene is present in a gene cluster with several related zinc finger genes in the pericentromeric region of chromosome 10. Pseudogenes have been identified on chromosomes 7 and 10. Alternative splicing results in multiple transcript variants.

Source: NCBI Gene 7582 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 132 total
• MANE Select transcript: NM_006955

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:13097
Approved symbol	ZNF33B
Name	zinc finger protein 33B
Location	10q11.21
Locus type	gene with protein product
Status	Approved
Aliases	KOX31, KOX2
Ensembl gene	ENSG00000196693
Ensembl biotype	protein_coding
OMIM	194522
Entrez	7582

Gene structure

Transcript identifiers

Ensembl transcripts: 13 — 10 protein_coding, 2 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000359467, ENST00000462075, ENST00000465206, ENST00000476796, ENST00000486187, ENST00000486614, ENST00000493285, ENST00000613419, ENST00000876414, ENST00000876415, ENST00000876416, ENST00000876417, ENST00000929479

RefSeq mRNA: 8 — MANE Select: NM_006955 NM_001305033, NM_001305035, NM_001305036, NM_001305037, NM_001305038, NM_001305039, NM_001305040, NM_006955

CCDS: CCDS7198

Canonical transcript exons

ENST00000359467 — 5 exons

Exon	Start	End
ENSE00001426426	42638474	42638570
ENSE00003471175	42632295	42632439
ENSE00003630553	42636920	42636972
ENSE00003789446	42631929	42632024
ENSE00003846000	42589109	42594699

Expression profiles

Bgee: expression breadth ubiquitous, 235 present calls, max score 94.53.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 8.3449 / max 162.6621, expressed in 1614 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
109151	4.1522	1346
109150	3.0249	1273
109152	0.8368	432
205825	0.2186	91
109148	0.0594	14
109149	0.0531	12

Top tissues by expression

282 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
body of pancreas	UBERON:0001150	94.53	gold quality
cortical plate	UBERON:0005343	93.84	gold quality
pancreas	UBERON:0001264	90.69	gold quality
right uterine tube	UBERON:0001302	89.40	gold quality
ganglionic eminence	UBERON:0004023	89.28	gold quality
left ovary	UBERON:0002119	87.81	gold quality
islet of Langerhans	UBERON:0000006	87.45	gold quality
buccal mucosa cell	CL:0002336	86.48	gold quality
cerebellar hemisphere	UBERON:0002245	86.45	gold quality
germinal epithelium of ovary	UBERON:0001304	86.30	gold quality
cerebellar cortex	UBERON:0002129	86.26	gold quality
minor salivary gland	UBERON:0001830	86.19	gold quality
calcaneal tendon	UBERON:0003701	85.97	gold quality
right hemisphere of cerebellum	UBERON:0014890	85.76	gold quality
saliva-secreting gland	UBERON:0001044	85.74	gold quality
ovary	UBERON:0000992	85.25	gold quality
parotid gland	UBERON:0001831	85.23	gold quality
ventricular zone	UBERON:0003053	85.21	gold quality
right ovary	UBERON:0002118	85.14	gold quality
adrenal tissue	UBERON:0018303	85.13	gold quality
rectum	UBERON:0001052	84.78	gold quality
body of stomach	UBERON:0001161	83.97	gold quality
left lobe of thyroid gland	UBERON:0001120	83.89	gold quality
metanephros cortex	UBERON:0010533	83.84	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	83.68	gold quality
cerebellum	UBERON:0002037	83.54	gold quality
thyroid gland	UBERON:0002046	83.54	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	83.51	gold quality
right lobe of thyroid gland	UBERON:0001119	83.49	gold quality
mouth mucosa	UBERON:0003729	83.24	gold quality

Single-cell (SCXA)

Detected in 4 experiment(s), a significant marker in 4.

Experiment	Marker?	Max mean expression
E-MTAB-8498	yes	854.86
E-ANND-3	yes	15.54
E-MTAB-6678	yes	10.63
E-MTAB-9801	yes	6.84

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): STAT1

miRNA regulators (miRDB)

119 targeting ZNF33B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-126-5P	100.00	72.71	3180
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-4262	100.00	73.26	3931
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-590-3P	99.96	74.34	6478
HSA-MIR-493-5P	99.96	72.47	2382
HSA-MIR-548J-3P	99.94	72.61	4881
HSA-MIR-101-3P	99.94	75.03	2230
HSA-MIR-548AE-3P	99.93	72.66	4867
HSA-MIR-548AH-3P	99.93	72.54	4872
HSA-MIR-548AM-3P	99.93	72.54	4872
HSA-MIR-548AQ-3P	99.93	72.66	4867
HSA-MIR-3682-5P	99.93	67.97	1163
HSA-MIR-10527-5P	99.91	72.28	3754
HSA-MIR-3686	99.90	70.53	2432
HSA-MIR-153-5P	99.89	73.86	6317
HSA-MIR-579-3P	99.86	71.66	3628
HSA-MIR-664B-3P	99.84	71.65	3590
HSA-MIR-4698	99.84	71.41	4303
HSA-MIR-4420	99.82	70.08	1624
HSA-MIR-181B-2-3P	99.81	70.06	1646
HSA-MIR-181B-3P	99.81	70.06	1646
HSA-MIR-374C-5P	99.80	72.06	2910

Literature-anchored findings (GeneRIF, showing 1)

• Zinc finger protein 33B demonstrates sex interaction with atopy-related markers in childhood asthma. (PMID:35953101)

Cross-species orthologs

0 orthologs

Paralogs (176): ZNF195 (ENSG00000005801), ZNF112 (ENSG00000062370), ZNF275 (ENSG00000063587), ZNF37A (ENSG00000075407), ZNF510 (ENSG00000081386), ZNF506 (ENSG00000081665), ZNF268 (ENSG00000090612), MZF1 (ENSG00000099326), ZNF629 (ENSG00000102870), ZNF175 (ENSG00000105497), ZNF85 (ENSG00000105750), ZFP30 (ENSG00000120784), ZNF45 (ENSG00000124459), ZNF391 (ENSG00000124613), ZNF436 (ENSG00000125945), ZNF484 (ENSG00000127081), ZNF835 (ENSG00000127903), ZNF780B (ENSG00000128000), ZSCAN10 (ENSG00000130182), ZNF317 (ENSG00000130803), ZNF331 (ENSG00000130844), ZNF227 (ENSG00000131115), ZNF141 (ENSG00000131127), ZNF132 (ENSG00000131849), ZNF189 (ENSG00000136870), ZIM3 (ENSG00000141946), ZFP14 (ENSG00000142065), ZNF514 (ENSG00000144026), ZNF300 (ENSG00000145908), RBAK (ENSG00000146587), ZNF157 (ENSG00000147117), ZNF182 (ENSG00000147118), ZNF41 (ENSG00000147124), ZNF7 (ENSG00000147789), ZNF117 (ENSG00000152926), ZNF221 (ENSG00000159905), ZNF235 (ENSG00000159917), ZNF714 (ENSG00000160352), ZNF577 (ENSG00000161551), ZNF12 (ENSG00000164631)

Protein

Protein identifiers

Zinc finger protein 33B — Q06732 (reviewed: Q06732)

Alternative names: Zinc finger protein 11B, Zinc finger protein KOX2

All UniProt accessions (5): Q06732, A0A087WVA3, A0A087WXF4, A0A087WXT3, A0A087X1D4

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (8): NP_001291962, NP_001291964, NP_001291965, NP_001291966, NP_001291967, NP_001291968, NP_001291969, NP_008886* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050826	Krueppel_C2H2_ZnFinger	Family

Pfam: PF00096, PF01352

UniProt features (27 total): zinc finger region 16, cross-link 5, sequence variant 2, sequence conflict 2, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q06732-F1	65.80	0.10

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (5): 181, 238, 313, 677, 733

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 82 (showing top): MORF_MSH3, MORF_BRCA1, LINDGREN_BLADDER_CANCER_CLUSTER_3_DN, MORF_RAD51L3, MORF_ATF2, MORF_PPP2R5B, KRIGE_RESPONSE_TO_TOSEDOSTAT_24HR_UP, MORF_MYL3, GAZDA_DIAMOND_BLACKFAN_ANEMIA_ERYTHROID_DN, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, MORF_IL16, MORF_DMPK, MORF_ERCC4, MORF_TFDP2, GOBP_NEGATIVE_REGULATION_OF_TRANSCRIPTION_BY_RNA_POLYMERASE_II

GO Biological Process (4): regulation of transcription by RNA polymerase II (GO:0006357), regulation of DNA-templated transcription (GO:0006355), regulation of macromolecule metabolic process (GO:0060255), regulation of primary metabolic process (GO:0080090)

GO Molecular Function (5): zinc ion binding (GO:0008270), sequence-specific double-stranded DNA binding (GO:1990837), DNA binding (GO:0003677), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of metabolic process	2
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
macromolecule metabolic process	1
primary metabolic process	1
transition metal ion binding	1
double-stranded DNA binding	1
sequence-specific DNA binding	1
nucleic acid binding	1
binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

306 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF33B	LHFPL7	Q6ICI0	587
ZNF33B	LRRIQ3	A6PVS8	399
ZNF33B	CCDC60	Q8IWA6	390
ZNF33B	PPP2R5C	Q13362	375
ZNF33B	VMA22	Q96NT0	372
ZNF33B	HES7	Q9BYE0	372
ZNF33B	KRTAP1-5	Q9BYS1	370
ZNF33B	EVX1	P49640	321
ZNF33B	MALRD1	Q5VYJ5	320
ZNF33B	FNBP4	Q8N3X1	312
ZNF33B	SLX4IP	Q5VYV7	305
ZNF33B	FPGT	O14772	305
ZNF33B	CHRM3	P20309	304
ZNF33B	KRTAP1-1	Q07627	274
ZNF33B	V9GXZ4	V9GXZ4	263

IntAct

13 interactions, top by confidence:

A	B	Type	Score
MAGEA12	ZNF33B	psi-mi:“MI:0915”(physical association)	0.560
ZNF33B	MAGEA12	psi-mi:“MI:0915”(physical association)	0.560
CBX6	IGF2BP3	psi-mi:“MI:0914”(association)	0.530
TRIM28	ZNF320	psi-mi:“MI:0914”(association)	0.530
ZNF33B	Hap1	psi-mi:“MI:0915”(physical association)	0.370
ZNF33B	ZHX1	psi-mi:“MI:0915”(physical association)	0.370
TRIM28	ZNF320	psi-mi:“MI:0914”(association)	0.350
FMR1	ZNF33B	psi-mi:“MI:0915”(physical association)	0.000
ZNF33B	HAP1	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (8): ZNF33B (Two-hybrid), ZNF33B (Affinity Capture-MS), ZNF33B (Affinity Capture-RNA), ZNF33B (Affinity Capture-MS), ZNF33B (Affinity Capture-MS), ZNF33B (Affinity Capture-MS), HAP1 (Two-hybrid), ZHX1 (Two-hybrid)

ESM2 similar proteins: A2VDP4, A6NHJ4, O94892, P0CJ79, P17014, P17021, P17025, P17032, P17035, P18733, P51508, P51814, Q06730, Q06732, Q09FC8, Q0VGE8, Q14586, Q32M78, Q3MIS6, Q5JVG2, Q5R4K8, Q5R9S5, Q5RBQ3, Q5RCJ2, Q5RER9, Q5TYW1, Q5VIY5, Q6P560, Q6PDB4, Q6ZMW2, Q6ZN06, Q6ZNA1, Q76KX8, Q7L2R6, Q86Y25, Q8N184, Q8N823, Q8N883, Q8N8J6, Q8NEP9

Diamond homologs: A0A1W2PQL4, A0JNB1, A0JPL0, A6NK53, A6QLU5, A6QPT6, A7MBI1, A8MT65, A8MUV8, A8MWA4, B2RXC5, B4DU55, B4DX44, E9PYI1, O14628, O75346, P0CH99, P0CI00, P17014, P17030, P17032, P17098, P51786, P85977, Q02386, Q06730, Q06732, Q0VAW7, Q12901, Q13360, Q14586, Q14588, Q14590, Q16587, Q2M3X9, Q2VY69, Q32M78, Q3ZCX4, Q49AA0, Q4R6J4

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

132 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	124
Likely benign	4
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

1798 predictions. Top by Δscore:

Variant	Effect	Δscore
10:42632294:CCCA:C	donor_gain	1.0000
10:42632297:A:AC	donor_gain	1.0000
10:42632298:C:CC	donor_gain	1.0000
10:42633253:A:AC	donor_gain	1.0000
10:42633254:C:CC	donor_gain	1.0000
10:42636970:CAG:C	acceptor_gain	1.0000
10:42636978:T:C	acceptor_gain	1.0000
10:42636978:T:TC	acceptor_gain	1.0000
10:42638468:GCTTA:G	donor_loss	1.0000
10:42638469:CTTAC:C	donor_loss	1.0000
10:42638471:TA:T	donor_loss	1.0000
10:42638472:A:AC	donor_gain	1.0000
10:42638472:A:AT	donor_loss	1.0000
10:42638473:C:CC	donor_gain	1.0000
10:42638473:CCT:C	donor_gain	1.0000
10:42638473:CCTCA:C	donor_gain	1.0000
10:42594697:CTT:C	acceptor_gain	0.9900
10:42631924:CCCA:C	donor_loss	0.9900
10:42631926:CAC:C	donor_loss	0.9900
10:42631927:A:AT	donor_loss	0.9900
10:42631928:CC:C	donor_loss	0.9900
10:42631955:T:TA	donor_gain	0.9900
10:42632021:TACC:T	acceptor_gain	0.9900
10:42632023:CC:C	acceptor_gain	0.9900
10:42632024:CC:C	acceptor_gain	0.9900
10:42632850:T:TA	donor_gain	0.9900
10:42633241:TGGA:T	donor_gain	0.9900
10:42636915:CTTA:C	donor_loss	0.9900
10:42636916:TTAC:T	donor_loss	0.9900
10:42636917:TACCT:T	donor_loss	0.9900

AlphaMissense

5227 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
10:42593600:G:C	F450L	0.999
10:42593600:G:T	F450L	0.999
10:42593602:A:G	F450L	0.999
10:42592844:G:C	F702L	0.998
10:42592844:G:T	F702L	0.998
10:42592846:A:G	F702L	0.998
10:42593096:G:C	F618L	0.998
10:42593096:G:T	F618L	0.998
10:42593098:A:G	F618L	0.998
10:42593331:A:G	L540P	0.998
10:42593432:G:C	F506L	0.998
10:42593432:G:T	F506L	0.998
10:42593434:A:G	F506L	0.998
10:42593499:A:G	L484P	0.998
10:42593516:A:C	F478L	0.998
10:42593516:A:T	F478L	0.998
10:42593518:A:G	F478L	0.998
10:42593583:A:G	L456P	0.998
10:42593768:A:C	F394L	0.998
10:42593768:A:T	F394L	0.998
10:42593770:A:G	F394L	0.998
10:42593079:A:G	L624P	0.997
10:42593163:A:G	L596P	0.997
10:42593247:A:G	L568P	0.997
10:42593348:G:C	F534L	0.997
10:42593348:G:T	F534L	0.997
10:42593350:A:G	F534L	0.997
10:42593667:A:G	L428P	0.997
10:42593679:T:G	Q424P	0.997
10:42593684:A:C	F422L	0.997

dbSNP variants (sampled 300 via entrez): RS1000003382 (10:42629870 T>C), RS1000007341 (10:42588827 T>C), RS1000059373 (10:42623481 T>C), RS1000137349 (10:42620689 G>A), RS1000147841 (10:42589119 A>G), RS1000257224 (10:42624060 G>A), RS1000266161 (10:42617453 T>C), RS1000284200 (10:42639657 G>A), RS1000394011 (10:42594865 G>C), RS1000454819 (10:42625049 T>C), RS1000509670 (10:42584543 CAG>C), RS1000550985 (10:42630176 A>AT), RS1000563730 (10:42579834 C>T), RS1000577188 (10:42583434 C>A), RS1000740886 (10:42619304 C>T)

Disease associations

OMIM: gene MIM:194522 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST004025_10	Systemic juvenile idiopathic arthritis	1.000000e-06
GCST007272_23	Pulse pressure	3.000000e-44

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0005763	pulse pressure measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

39 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression, affects cotreatment, increases abundance, increases expression	3
Resveratrol	affects cotreatment, increases expression, decreases expression	2
Tobacco Smoke Pollution	decreases expression	2
Valproic Acid	decreases expression	2
Cyclosporine	increases expression	2
Cadmium Chloride	decreases expression, increases expression	2
aristolochic acid I	decreases expression	1
dicrotophos	decreases expression	1
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
trichostatin A	affects expression	1
sulforaphane	decreases expression	1
butyraldehyde	decreases expression	1
manganese chloride	increases abundance, increases expression	1
coumarin	decreases phosphorylation	1
abrine	decreases expression	1
2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine	increases expression, increases response to substance	1
jinfukang	affects cotreatment, increases expression	1
NSC 689534	affects binding, increases expression	1
Sunitinib	increases expression	1
Arsenic Trioxide	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Ethanol	decreases expression	1
Arsenic	increases expression, affects cotreatment, increases abundance	1
Cisplatin	increases expression, affects cotreatment	1
Coal	decreases expression, increases abundance	1
Copper	affects binding, increases expression	1
Manganese	increases abundance, increases expression	1
Methyl Methanesulfonate	increases expression	1
Nickel	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.