ZNF433

Summary

ZNF433 (zinc finger protein 433, HGNC:20811) is a protein-coding gene on chromosome 19p13.2, encoding Zinc finger protein 433 (Q8N7K0). May be involved in transcriptional regulation.

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.

Source: NCBI Gene 163059 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 99 total
• MANE Select transcript: NM_001308348

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:20811
Approved symbol	ZNF433
Name	zinc finger protein 433
Location	19p13.2
Locus type	gene with protein product
Status	Approved
Aliases	FLJ40981
Ensembl gene	ENSG00000197647
Ensembl biotype	protein_coding
Entrez	163059

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 10 protein_coding, 1 nonsense_mediated_decay

ENST00000344980, ENST00000411841, ENST00000419886, ENST00000455504, ENST00000478765, ENST00000547560, ENST00000548669, ENST00000550507, ENST00000550745, ENST00000552904, ENST00000870917

RefSeq mRNA: 6 — MANE Select: NM_001308348 NM_001080411, NM_001308346, NM_001308348, NM_001308351, NM_001308355, NM_001308357

CCDS: CCDS45983, CCDS77240, CCDS92523, CCDS92525

Canonical transcript exons

ENST00000550507 — 4 exons

Exon	Start	End
ENSE00002367905	12035537	12035679
ENSE00003646414	12017876	12017936
ENSE00003663715	12018166	12018292
ENSE00003930179	12014715	12016666

Expression profiles

Bgee: expression breadth ubiquitous, 134 present calls, max score 88.47.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 3.5126 / max 81.7610, expressed in 1262 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
179284	3.5126	1262

Top tissues by expression

134 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right testis	UBERON:0004534	88.47	gold quality
left testis	UBERON:0004533	88.11	gold quality
testis	UBERON:0000473	87.86	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.17	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	85.87	gold quality
endometrium	UBERON:0001295	82.51	gold quality
ventricular zone	UBERON:0003053	81.57	gold quality
calcaneal tendon	UBERON:0003701	81.54	gold quality
body of pancreas	UBERON:0001150	81.52	gold quality
lower esophagus mucosa	UBERON:0035834	80.98	gold quality
kidney	UBERON:0002113	80.88	gold quality
pancreas	UBERON:0001264	80.75	gold quality
adult mammalian kidney	UBERON:0000082	80.35	gold quality
ganglionic eminence	UBERON:0004023	80.31	gold quality
cortex of kidney	UBERON:0001225	79.64	gold quality
cortical plate	UBERON:0005343	79.63	gold quality
right lobe of thyroid gland	UBERON:0001119	79.43	gold quality
islet of Langerhans	UBERON:0000006	79.41	gold quality
skeletal muscle tissue	UBERON:0001134	79.38	gold quality
metanephros cortex	UBERON:0010533	78.85	gold quality
thyroid gland	UBERON:0002046	78.77	gold quality
left lobe of thyroid gland	UBERON:0001120	78.36	gold quality
muscle tissue	UBERON:0002385	78.13	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	77.72	gold quality
esophagus mucosa	UBERON:0002469	77.68	gold quality
tonsil	UBERON:0002372	77.53	gold quality
apex of heart	UBERON:0002098	77.49	gold quality
bone marrow	UBERON:0002371	77.28	gold quality
urinary bladder	UBERON:0001255	77.02	gold quality
hindlimb stylopod muscle	UBERON:0004252	76.97	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	3.75

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 2)

• ZNF433 has been identified as a candidate gene in a genome-wide association study of German multiple sclerosis patients. (PMID:20598377)
• The Identification of Zinc-Finger Protein 433 as a Possible Prognostic Biomarker for Clear-Cell Renal Cell Carcinoma. (PMID:34439859)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Zfp961	ENSMUSG00000052446
rattus_norvegicus	Zfp617	ENSRNOG00000049856

Paralogs (15): ZNF14 (ENSG00000105708), ZNF57 (ENSG00000171970), ZNF791 (ENSG00000173875), ZNF443 (ENSG00000180855), ZNF101 (ENSG00000181896), ZNF563 (ENSG00000188868), ZNF799 (ENSG00000196466), ZNF700 (ENSG00000196757), ZNF441 (ENSG00000197044), ZNF44 (ENSG00000197857), ZNF823 (ENSG00000197933), ZNF442 (ENSG00000198342), ZNF844 (ENSG00000223547), ZNF709 (ENSG00000242852), ZNF878 (ENSG00000257446)

Protein

Protein identifiers

Zinc finger protein 433 — Q8N7K0 (reviewed: Q8N7K0)

All UniProt accessions (8): C9JQA6, C9JUV2, Q8N7K0, F8VP42, F8VTV7, F8VXJ9, F8W0C9, F8W652

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q8N7K0-1	1	yes
Q8N7K0-2	2

RefSeq proteins (6): NP_001073880, NP_001295275, NP_001295277, NP_001295280, NP_001295284, NP_001295286 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily

Pfam: PF00096, PF01352, PF13465

UniProt features (22 total): zinc finger region 19, chain 1, domain 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8N7K0-F1	74.67	0.07

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 14 (showing top): chr19p13, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, GLI4_TARGET_GENES, ZNF22_TARGET_GENES, ZNF350_TARGET_GENES, DESCARTES_MAIN_FETAL_CSH1_CSH2_POSITIVE_CELLS, INSM2_TARGET_GENES, OSMAN_BLOOD_CHAD63_KH_AGE_18_50YO_HIGH_DOSE_SUBJECTS_24HR_DN, GOMF_DNA_BINDING_TRANSCRIPTION_FACTOR_ACTIVITY, PULVER_FOREY_CELLCYCLE_PEAKING_G1_S, GOMF_RNA_POLYMERASE_II_TRANSCRIPTION_REGULATORY_REGION_SEQUENCE_SPECIFIC_DNA_BINDING, MCCABE_BOUND_BY_HOXC6, REACTOME_RNA_POLYMERASE_II_TRANSCRIPTION

GO Biological Process (2): regulation of transcription by RNA polymerase II (GO:0006357), regulation of DNA-templated transcription (GO:0006355)

GO Molecular Function (7): RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), protein binding (GO:0005515), metal ion binding (GO:0046872), sequence-specific double-stranded DNA binding (GO:1990837)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
transcription cis-regulatory region binding	1
chromatin	1
RNA polymerase II transcription regulatory region sequence-specific DNA binding	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
binding	1
cation binding	1
double-stranded DNA binding	1
sequence-specific DNA binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

278 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF433	TMEM220	Q6QAJ8	475
ZNF433	SPATA31E1	Q6ZUB1	447
ZNF433	OR2D3	Q8NGH3	432
ZNF433	RCCD1	A6NED2	420
ZNF433	THYN1	Q9P016	391
ZNF433	CYLC2	Q14093	372
ZNF433	CSNK1G3	Q9Y6M4	358
ZNF433	OLFML2B	Q68BL8	353
ZNF433	SPMIP9	Q96LM6	348
ZNF433	SPATA24	Q86W54	330
ZNF433	SPATC1	Q76KD6	323
ZNF433	DNAH7	Q8WXX0	320
ZNF433	MOSPD1	Q9UJG1	314
ZNF433	FHAD1	B1AJZ9	313
ZNF433	PTGDR	Q13258	311

IntAct

23 interactions, top by confidence:

A	B	Type	Score
ZNF433	KRTAP10-5	psi-mi:“MI:0915”(physical association)	0.560
ZNF433	KRTAP10-7	psi-mi:“MI:0915”(physical association)	0.560
ZNF433	MDFI	psi-mi:“MI:0915”(physical association)	0.560
ZNF433		psi-mi:“MI:0915”(physical association)	0.560
KRTAP10-5	ZNF433	psi-mi:“MI:0915”(physical association)	0.560
KRTAP10-7	ZNF433	psi-mi:“MI:0915”(physical association)	0.560
MDFI	ZNF433	psi-mi:“MI:0915”(physical association)	0.560
ZNF433	CEP70	psi-mi:“MI:0915”(physical association)	0.560
KRTAP10-9	ZNF433	psi-mi:“MI:0915”(physical association)	0.560
ZNF433	ACTA2	psi-mi:“MI:0914”(association)	0.350
ZNF433	CEP70	psi-mi:“MI:0915”(physical association)	0.000
ZNF433	KRTAP10-9	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (13): ZNF433 (Two-hybrid), KRTAP10-7 (Two-hybrid), KRTAP10-1 (Two-hybrid), KRTAP10-5 (Two-hybrid), KRTAP10-3 (Two-hybrid), ACTA2 (Affinity Capture-MS), ANKHD1 (Affinity Capture-MS), ZNF433 (Two-hybrid), KRTAP10-9 (Two-hybrid), ANKHD1 (Affinity Capture-MS), ACTA2 (Affinity Capture-MS), KRTAP10-5 (Two-hybrid), KRTAP10-7 (Two-hybrid)

ESM2 similar proteins: A2RRD8, A6NHJ4, A6NK75, B4DU55, O75346, P0DPD5, P15621, P17030, P17039, P52737, Q0VGE8, Q14588, Q147U1, Q15928, Q2M3X9, Q3KNS6, Q494X3, Q4R6C2, Q5HYK9, Q5R5Q6, Q5R5S6, Q5REA0, Q5REK1, Q60585, Q61751, Q6ECI4, Q6P9Y7, Q6ZN19, Q6ZNA1, Q7Z3V5, Q86T29, Q86WZ6, Q8N141, Q8N7K0, Q8N9F8, Q8NA42, Q8NCK3, Q8NDQ6, Q8NEM1, Q8WV37

Diamond homologs: A0JPK3, A8MT65, C9JN71, E9QAG8, G3X9G7, O75820, P16373, P16374, P16415, P17017, P17024, P17025, P51815, P52737, Q08AG5, Q08ER8, Q0D2J5, Q15973, Q2M218, Q3KP31, Q3V080, Q494X3, Q4R4C7, Q5MYW4, Q5R9F0, Q5R9S5, Q5REI6, Q5REK1, Q68EA5, Q6P560, Q6P5C7, Q6ZQV5, Q7L945, Q86T29, Q8BGV5, Q8C6P8, Q8IYI8, Q8IZC7, Q8N7K0, Q8N972

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

99 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	94
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

970 predictions. Top by Δscore:

Variant	Effect	Δscore
19:12016662:CAATT:C	acceptor_gain	1.0000
19:12016664:ATT:A	acceptor_gain	1.0000
19:12016665:TT:T	acceptor_gain	1.0000
19:12016665:TTC:T	acceptor_loss	1.0000
19:12016666:TCTG:T	acceptor_loss	1.0000
19:12016667:C:CC	acceptor_gain	1.0000
19:12018304:CACA:C	acceptor_gain	1.0000
19:12018307:A:C	acceptor_gain	1.0000
19:12016663:AATT:A	acceptor_gain	0.9900
19:12016668:T:A	acceptor_loss	0.9900
19:12016674:C:CT	acceptor_gain	0.9900
19:12018160:CCTTA:C	donor_loss	0.9900
19:12018161:CTTA:C	donor_loss	0.9900
19:12018162:TTAC:T	donor_loss	0.9900
19:12018163:TACCT:T	donor_loss	0.9900
19:12018165:C:CG	donor_loss	0.9900
19:12018306:CA:C	acceptor_gain	0.9900
19:12018309:G:C	acceptor_gain	0.9900
19:12018311:A:C	acceptor_gain	0.9900
19:12035533:TCAC:T	donor_loss	0.9900
19:12035534:CACCA:C	donor_loss	0.9900
19:12035535:ACCAT:A	donor_loss	0.9900
19:12035536:C:CT	donor_loss	0.9900
19:12017935:CC:C	acceptor_gain	0.9800
19:12017936:CC:C	acceptor_gain	0.9800
19:12017937:C:CC	acceptor_gain	0.9800
19:12034827:CA:C	donor_gain	0.9800
19:12035531:GCTCA:G	donor_loss	0.9800
19:12016669:G:GC	acceptor_gain	0.9700
19:12016675:A:T	acceptor_gain	0.9700

AlphaMissense

4454 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:12014986:A:C	F627L	0.991
19:12014986:A:T	F627L	0.991
19:12014988:A:G	F627L	0.991
19:12015154:A:C	F571L	0.991
19:12015154:A:T	F571L	0.991
19:12015156:A:G	F571L	0.991
19:12015070:A:C	F599L	0.990
19:12015070:A:T	F599L	0.990
19:12015072:A:G	F599L	0.990
19:12016078:G:C	F263L	0.990
19:12016078:G:T	F263L	0.990
19:12016080:A:G	F263L	0.990
19:12015994:G:C	F291L	0.986
19:12015994:G:T	F291L	0.986
19:12015996:A:G	F291L	0.986
19:12016162:A:C	F235L	0.984
19:12016162:A:T	F235L	0.984
19:12016164:A:G	F235L	0.984
19:12015238:G:C	F543L	0.983
19:12015238:G:T	F543L	0.983
19:12015240:A:G	F543L	0.983
19:12015574:G:C	F431L	0.981
19:12015574:G:T	F431L	0.981
19:12015576:A:G	F431L	0.981
19:12018257:G:C	F16L	0.975
19:12018257:G:T	F16L	0.975
19:12018259:A:G	F16L	0.975
19:12015199:G:C	H556Q	0.974
19:12015199:G:T	H556Q	0.974
19:12016039:G:C	H276Q	0.971

dbSNP variants (sampled 300 via entrez): RS1000146377 (19:12017194 GT>G,GTT), RS1000341634 (19:12029911 C>T), RS1000348481 (19:12035664 G>A), RS1000471168 (19:12031364 T>C), RS1000754956 (19:12034622 T>C), RS1000863000 (19:12025056 T>C), RS1001194014 (19:12035083 T>G), RS1001226859 (19:12035352 C>G), RS1001409645 (19:12029240 A>C), RS1001456354 (19:12027688 A>C,G), RS1001529747 (19:12028048 A>C,G), RS1001749808 (19:12015308 G>C), RS1001815925 (19:12026141 G>C), RS1001826030 (19:12026449 G>T), RS1002025155 (19:12020449 A>C,G)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST000716_4	Multiple sclerosis	1.000000e-06

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

16 total (human), top 16 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression, increases expression	2
aristolochic acid I	increases expression	1
bisphenol A	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
aflatoxin B2	increases methylation	1
Leflunomide	increases expression	1
Acetaminophen	increases expression	1
Air Pollutants	increases abundance, increases expression	1
Benzo(a)pyrene	decreases methylation	1
Cisplatin	decreases expression	1
N-Nitrosopyrrolidine	increases expression	1
Smoke	decreases expression	1
Urethane	increases expression	1
Cadmium Chloride	increases expression	1
Acrylamide	increases expression	1
Particulate Matter	increases abundance, increases expression	1

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_XW46	HEK293 eGFP-ZNF433	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.