ZNF443

Summary

ZNF443 (zinc finger protein 443, HGNC:20878) is a protein-coding gene on chromosome 19p13.2, encoding Zinc finger protein 443 (Q9Y2A4). May be involved in transcriptional regulation.

Zinc finger proteins (ZNFs) bind DNA and, through this binding, regulate gene transcription. Most ZNFs contain conserved C2H2 motifs and are classified as Kruppel-type zinc fingers. For a general description of these proteins, see ZNF91 (MIM 603971).

Source: NCBI Gene 10224 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 140 total
• MANE Select transcript: NM_005815

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:20878
Approved symbol	ZNF443
Name	zinc finger protein 443
Location	19p13.2
Locus type	gene with protein product
Status	Approved
Aliases	ZK1
Ensembl gene	ENSG00000180855
Ensembl biotype	protein_coding
OMIM	606697
Entrez	10224

Gene structure

Transcript identifiers

Ensembl transcripts: 2 — 1 protein_coding, 1 nonsense_mediated_decay

ENST00000301547, ENST00000436821

RefSeq mRNA: 1 — MANE Select: NM_005815 NM_005815

CCDS: CCDS32918

Canonical transcript exons

ENST00000301547 — 4 exons

Exon	Start	End
ENSE00001117451	12432377	12432437
ENSE00001129820	12429706	12431980
ENSE00001484733	12440912	12441021
ENSE00001729158	12433071	12433197

Expression profiles

Bgee: expression breadth ubiquitous, 133 present calls, max score 81.54.

FANTOM5 (CAGE): breadth broad, TPM avg 2.6058 / max 113.8635, expressed in 890 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
179308	1.3779	610
179309	0.7910	428
179310	0.4368	270

Top tissues by expression

136 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	81.54	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	80.38	gold quality
endometrium	UBERON:0001295	79.60	gold quality
ganglionic eminence	UBERON:0004023	78.61	gold quality
ventricular zone	UBERON:0003053	77.20	gold quality
calcaneal tendon	UBERON:0003701	77.12	gold quality
cortical plate	UBERON:0005343	74.97	gold quality
corpus callosum	UBERON:0002336	73.16	gold quality
mucosa of transverse colon	UBERON:0004991	72.83	gold quality
rectum	UBERON:0001052	72.48	gold quality
cortex of kidney	UBERON:0001225	71.79	gold quality
placenta	UBERON:0001987	71.47	gold quality
metanephros cortex	UBERON:0010533	70.93	gold quality
stromal cell of endometrium	CL:0002255	70.59	gold quality
right lobe of liver	UBERON:0001114	70.39	gold quality
caudate nucleus	UBERON:0001873	70.18	gold quality
kidney	UBERON:0002113	70.14	gold quality
nucleus accumbens	UBERON:0001882	69.95	gold quality
transverse colon	UBERON:0001157	69.76	gold quality
urinary bladder	UBERON:0001255	69.74	gold quality
adult mammalian kidney	UBERON:0000082	69.49	gold quality
smooth muscle tissue	UBERON:0001135	69.46	gold quality
putamen	UBERON:0001874	69.46	gold quality
tonsil	UBERON:0002372	69.35	gold quality
myometrium	UBERON:0001296	69.28	gold quality
esophagus mucosa	UBERON:0002469	69.08	gold quality
right adrenal gland cortex	UBERON:0035827	69.05	gold quality
colon	UBERON:0001155	69.02	gold quality
minor salivary gland	UBERON:0001830	68.98	gold quality
body of pancreas	UBERON:0001150	68.95	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.16

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

47 targeting ZNF443, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4262	100.00	73.26	3931
HSA-MIR-188-3P	100.00	68.76	1240
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-513A-5P	100.00	69.77	2465
HSA-MIR-4500	99.99	72.72	2367
HSA-MIR-6759-5P	99.99	66.54	785
HSA-MIR-181A-5P	99.99	72.96	2995
HSA-MIR-181B-5P	99.99	72.97	2996
HSA-MIR-181C-5P	99.99	72.95	2996
HSA-MIR-181D-5P	99.99	73.04	2997
HSA-LET-7A-5P	99.98	72.29	1790
HSA-LET-7B-5P	99.98	72.31	1790
HSA-LET-7C-5P	99.98	72.29	1790
HSA-LET-7E-5P	99.98	72.29	1790
HSA-LET-7F-5P	99.98	72.56	1784
HSA-LET-7G-5P	99.98	72.37	1784
HSA-LET-7I-5P	99.98	72.37	1788
HSA-MIR-98-5P	99.98	72.33	1787
HSA-LET-7D-5P	99.96	71.76	1632
HSA-MIR-4458	99.96	71.64	1650
HSA-MIR-381-3P	99.93	71.87	2854
HSA-MIR-515-5P	99.92	69.82	2343
HSA-MIR-519E-5P	99.92	69.62	2358
HSA-MIR-300	99.92	71.76	2856
HSA-MIR-202-3P	99.84	71.41	1290
HSA-MIR-3680-3P	99.75	72.51	3095
HSA-MIR-1284	99.67	73.56	1353
HSA-MIR-5700	99.64	69.88	2280
HSA-MIR-510-3P	99.54	70.06	2965
HSA-MIR-217-5P	99.49	69.93	1419

Literature-anchored findings (GeneRIF, showing 1)

• The gene expression of ZK1 was down-regulated during early apoptosis of human hepatoma cells exposed to Paeoniae Radix extract in vitro. (PMID:12215374)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Zfp961	ENSMUSG00000052446
rattus_norvegicus	Zfp617	ENSRNOG00000049856

Paralogs (15): ZNF14 (ENSG00000105708), ZNF57 (ENSG00000171970), ZNF791 (ENSG00000173875), ZNF101 (ENSG00000181896), ZNF563 (ENSG00000188868), ZNF799 (ENSG00000196466), ZNF700 (ENSG00000196757), ZNF441 (ENSG00000197044), ZNF433 (ENSG00000197647), ZNF44 (ENSG00000197857), ZNF823 (ENSG00000197933), ZNF442 (ENSG00000198342), ZNF844 (ENSG00000223547), ZNF709 (ENSG00000242852), ZNF878 (ENSG00000257446)

Protein

Protein identifiers

Zinc finger protein 443 — Q9Y2A4 (reviewed: Q9Y2A4)

Alternative names: Krueppel-type zinc finger protein ZK1

All UniProt accessions (2): Q9Y2A4, F8WDY2

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Induction. Induced following exposure to ionizing radiation.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (1): NP_005806* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050589	Ikaros_C2H2-ZF	Family

Pfam: PF00096, PF01352, PF13912

UniProt features (28 total): zinc finger region 19, sequence variant 5, sequence conflict 2, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9Y2A4-F1	73.13	0.10

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 63 (showing top): BROWNE_HCMV_INFECTION_12HR_UP, SHEDDEN_LUNG_CANCER_GOOD_SURVIVAL_A5, WALLACE_PROSTATE_CANCER_RACE_DN, chr19p13, GEORGES_TARGETS_OF_MIR192_AND_MIR215, HAMAI_APOPTOSIS_VIA_TRAIL_UP, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, MTOR_UP.N4.V1_DN, BLUM_RESPONSE_TO_SALIRASIB_DN, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, ALKBH3_TARGET_GENES, CEBPZ_TARGET_GENES, PRKDC_TARGET_GENES, ZBTB12_TARGET_GENES, ZNF350_TARGET_GENES

GO Biological Process (3): regulation of transcription by RNA polymerase II (GO:0006357), apoptotic process (GO:0006915), regulation of DNA-templated transcription (GO:0006355)

GO Molecular Function (5): RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
programmed cell death	1
apoptotic signaling pathway	1
execution phase of apoptosis	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
transcription cis-regulatory region binding	1
chromatin	1
RNA polymerase II transcription regulatory region sequence-specific DNA binding	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

400 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF443	ZPR1	O75312	593
ZNF443	TK1	P04183	425
ZNF443	IL3	P08700	387
ZNF443	FLRT2	O43155	373
ZNF443	SSH1	Q8WYL5	372
ZNF443	OR2T2	Q6IF00	371
ZNF443	GCNA	Q96QF7	362
ZNF443	USP30	Q70CQ3	361
ZNF443	ABTB1	Q969K4	358
ZNF443	Q6IQ01	Q6IQ01	348
ZNF443	COX11	Q9Y6N1	323
ZNF443	TPRG1L	Q5T0D9	323
ZNF443	GTF3C6	Q969F1	311
ZNF443	LCN10	Q6JVE6	305
ZNF443	DNAJC13	O75165	296

IntAct

8 interactions, top by confidence:

A	B	Type	Score
	ZNF443	psi-mi:“MI:0915”(physical association)	0.560
TRIM28	ZNF320	psi-mi:“MI:0914”(association)	0.530
ECE1	ZNF443	psi-mi:“MI:0915”(physical association)	0.370
BCL6	CACNA1A	psi-mi:“MI:0914”(association)	0.350
	ZNF443	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (3): ZNF443 (Two-hybrid), ZNF443 (Affinity Capture-MS), ZNF443 (Affinity Capture-MS)

ESM2 similar proteins: A6NDX5, A6NK75, A6NNF4, A6NP11, B4DXR9, B7Z6K7, C9JN71, O43361, O75290, O75373, P0CJ79, P0DKX0, P17019, P17021, P17035, P17039, P35789, Q02386, Q03938, Q08AN1, Q13106, Q15928, Q3ZCX4, Q5R9F0, Q5SXM1, Q68EA5, Q6P9A1, Q6PDB4, Q6ZN06, Q6ZN08, Q6ZNG1, Q76KX8, Q86V71, Q86XN6, Q8IYB9, Q8N4W9, Q8NEP9, Q8TBZ8, Q8TC21, Q8TF32

Diamond homologs: A0JPK3, A2RRD8, A2VDQ7, A3KN32, A8MUZ8, A8MWA4, C9JN71, E9QAG8, O60384, O60765, O75820, P0CH99, P0CI00, P10076, P10755, P15621, P16374, P16415, P17017, P17024, P51523, P52737, P52740, Q06730, Q06732, Q08AG5, Q0D2J5, Q13106, Q13360, Q15928, Q15935, Q15973, Q32M78, Q3KP31, Q4R4C7, Q4R6J4, Q4R882, Q5R9F0, Q5RC79, Q5REI6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

140 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	126
Likely benign	9
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

613 predictions. Top by Δscore:

Variant	Effect	Δscore
19:12433209:C:T	acceptor_gain	1.0000
19:12433209:CACA:C	acceptor_gain	1.0000
19:12433211:C:CT	acceptor_gain	1.0000
19:12433211:CATG:C	acceptor_gain	1.0000
19:12433212:A:C	acceptor_gain	1.0000
19:12433214:G:GC	acceptor_gain	1.0000
19:12433217:C:CT	acceptor_gain	1.0000
19:12431978:CAT:C	acceptor_gain	0.9900
19:12431981:C:CC	acceptor_gain	0.9900
19:12432433:CATTA:C	acceptor_gain	0.9900
19:12432438:C:CC	acceptor_gain	0.9900
19:12433065:CCTTA:C	donor_loss	0.9900
19:12433066:CTTA:C	donor_loss	0.9900
19:12433067:TTA:T	donor_loss	0.9900
19:12433068:T:TA	donor_loss	0.9900
19:12433069:A:AG	donor_loss	0.9900
19:12433070:CCTA:C	donor_gain	0.9900
19:12433208:CCACA:C	acceptor_gain	0.9900
19:12433218:A:T	acceptor_gain	0.9900
19:12440908:GCACC:G	donor_loss	0.9900
19:12440909:CAC:C	donor_loss	0.9900
19:12440910:A:T	donor_loss	0.9900
19:12440911:C:CG	donor_loss	0.9900
19:12440911:C:T	donor_loss	0.9900
19:12440924:T:TA	donor_gain	0.9900
19:12441001:T:TA	donor_gain	0.9900
19:12431978:CATCT:C	acceptor_loss	0.9800
19:12431980:TC:T	acceptor_loss	0.9800
19:12431981:CT:C	acceptor_loss	0.9800
19:12431982:T:G	acceptor_loss	0.9800

AlphaMissense

4494 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:12431218:G:C	F318L	0.991
19:12431218:G:T	F318L	0.991
19:12431220:A:G	F318L	0.991
19:12431134:A:C	F346L	0.987
19:12431134:A:T	F346L	0.987
19:12431136:A:G	F346L	0.987
19:12431050:A:C	F374L	0.985
19:12431050:A:T	F374L	0.985
19:12431052:A:G	F374L	0.985
19:12431386:A:C	F262L	0.985
19:12431386:A:T	F262L	0.985
19:12431388:A:G	F262L	0.985
19:12431470:A:C	F234L	0.985
19:12431470:A:T	F234L	0.985
19:12431472:A:G	F234L	0.985
19:12430549:G:C	F541L	0.979
19:12430549:G:T	F541L	0.979
19:12430551:A:G	F541L	0.979
19:12431302:G:C	F290L	0.979
19:12431302:G:T	F290L	0.979
19:12431304:A:G	F290L	0.979
19:12431107:G:C	H355Q	0.978
19:12431107:G:T	H355Q	0.978
19:12430465:G:C	F569L	0.977
19:12430465:G:T	F569L	0.977
19:12430467:A:G	F569L	0.977
19:12430882:A:C	F430L	0.977
19:12430882:A:T	F430L	0.977
19:12430884:A:G	F430L	0.977
19:12433162:G:C	F13L	0.971

dbSNP variants (sampled 300 via entrez): RS1000182615 (19:12429840 A>G,T), RS1000230849 (19:12430087 C>T), RS1000243704 (19:12435673 G>A), RS1000358992 (19:12435445 G>C), RS1000424547 (19:12440764 T>G), RS1001589343 (19:12440496 C>A,T), RS1001705562 (19:12440296 C>T), RS1002048968 (19:12434948 TGC>T), RS1002600530 (19:12439188 C>A), RS1002653348 (19:12433628 G>A,T), RS1002716907 (19:12438906 T>A), RS1003604138 (19:12438097 A>C), RS1003636684 (19:12442529 G>A,T), RS1003718750 (19:12437993 C>T), RS1003784544 (19:12442913 A>C,T)

Disease associations

OMIM: gene MIM:606697 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST010083_168	Hemoglobin levels	1.000000e-08

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004509	hemoglobin measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

27 total (human), top 27 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
3-((6-(2-methoxyphenyl)pyrimidin-4-yl)amino)phenyl)methane sulfonamide	decreases expression	1
TAK-243	increases sumoylation	1
testosterone enanthate	affects expression	1
triphenyl phosphate	affects expression	1
arsenite	affects binding, decreases reaction	1
potassium chromate(VI)	decreases expression	1
nickel sulfate	increases expression	1
di-n-butylphosphoric acid	affects expression	1
abrine	decreases expression	1
7-(benzylamino)-1,3,4,8-tetrahydropyrrolo(4,3,2-de)quinolin-8(1H)-one	decreases expression	1
Temozolomide	increases expression	1
Acetaminophen	increases expression	1
Benzo(a)pyrene	increases methylation	1
Cadmium	decreases expression	1
Cisplatin	increases expression	1
Demecolcine	decreases expression	1
Dietary Carbohydrates	increases expression	1
Estradiol	decreases expression	1
Formaldehyde	decreases expression	1
Menthol	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Valproic Acid	decreases expression	1
Vincristine	decreases expression	1
Vitamin E	decreases expression	1
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide	decreases expression	1
Sodium Selenite	increases expression	1
Cadmium Chloride	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.