ZNF577

Summary

ZNF577 (zinc finger protein 577, HGNC:28673) is a protein-coding gene on chromosome 19q13.41, encoding Zinc finger protein 577 (Q9BSK1). May be involved in transcriptional regulation.

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.

Source: NCBI Gene 84765 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 90 total
• MANE Select transcript: NM_001370449

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28673
Approved symbol	ZNF577
Name	zinc finger protein 577
Location	19q13.41
Locus type	gene with protein product
Status	Approved
Aliases	MGC4400
Ensembl gene	ENSG00000161551
Ensembl biotype	protein_coding
Entrez	84765

Gene structure

Transcript identifiers

Ensembl transcripts: 24 — 15 protein_coding, 6 protein_coding_CDS_not_defined, 2 retained_intron, 1 nonsense_mediated_decay

ENST00000301399, ENST00000451628, ENST00000638325, ENST00000638348, ENST00000638423, ENST00000638538, ENST00000638684, ENST00000638721, ENST00000638814, ENST00000638827, ENST00000638875, ENST00000639183, ENST00000639416, ENST00000639636, ENST00000639703, ENST00000640429, ENST00000640678, ENST00000640955, ENST00000879657, ENST00000879658, ENST00000879659, ENST00000879660, ENST00000953227, ENST00000953228

RefSeq mRNA: 10 — MANE Select: NM_001370449 NM_001135590, NM_001370447, NM_001370448, NM_001370449, NM_001370450, NM_001370452, NM_001370455, NM_001370456, NM_001370457, NM_032679

CCDS: CCDS12842, CCDS46160

Canonical transcript exons

ENST00000638348 — 6 exons

Exon	Start	End
ENSE00001214869	51880323	51880401
ENSE00001509763	51880679	51880877
ENSE00001592310	51867048	51873706
ENSE00003621432	51877282	51877377
ENSE00003787987	51878389	51878515
ENSE00003806162	51886821	51887951

Expression profiles

Bgee: expression breadth ubiquitous, 201 present calls, max score 94.01.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 4.0934 / max 130.1297, expressed in 1165 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
182504	3.3061	1067
182505	0.7872	428

Top tissues by expression

254 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	94.01	gold quality
right testis	UBERON:0004534	93.72	gold quality
left ovary	UBERON:0002119	93.47	gold quality
calcaneal tendon	UBERON:0003701	92.34	gold quality
right ovary	UBERON:0002118	92.13	gold quality
sural nerve	UBERON:0015488	91.18	gold quality
left lobe of thyroid gland	UBERON:0001120	90.42	gold quality
testis	UBERON:0000473	90.20	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	90.17	gold quality
right lobe of thyroid gland	UBERON:0001119	89.92	gold quality
thyroid gland	UBERON:0002046	89.39	gold quality
muscle layer of sigmoid colon	UBERON:0035805	88.33	gold quality
right uterine tube	UBERON:0001302	88.32	gold quality
apex of heart	UBERON:0002098	88.14	gold quality
lower esophagus muscularis layer	UBERON:0035833	87.74	gold quality
skin of abdomen	UBERON:0001416	87.73	gold quality
lower esophagus	UBERON:0013473	87.73	gold quality
tibial artery	UBERON:0007610	87.63	gold quality
popliteal artery	UBERON:0002250	87.62	gold quality
body of uterus	UBERON:0009853	87.43	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	87.17	gold quality
tibial nerve	UBERON:0001323	87.15	gold quality
minor salivary gland	UBERON:0001830	86.97	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.86	gold quality
buccal mucosa cell	CL:0002336	86.83	gold quality
ovary	UBERON:0000992	86.76	gold quality
lower esophagus mucosa	UBERON:0035834	86.69	gold quality
aorta	UBERON:0000947	86.56	gold quality
left uterine tube	UBERON:0001303	86.38	gold quality
metanephros cortex	UBERON:0010533	86.35	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	6.18

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

69 targeting ZNF577, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4510	100.00	66.60	2050
HSA-MIR-6127	100.00	66.76	2188
HSA-MIR-6129	100.00	66.46	2080
HSA-MIR-6130	100.00	66.69	2012
HSA-MIR-6133	100.00	66.48	2064
HSA-MIR-29A-3P	100.00	73.11	1835
HSA-MIR-29B-3P	100.00	73.18	1833
HSA-MIR-29C-3P	100.00	73.15	1833
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-27A-3P	99.98	72.13	2955
HSA-MIR-27B-3P	99.98	72.13	2955
HSA-MIR-9985	99.98	72.11	2939
HSA-MIR-512-3P	99.97	67.35	1049
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-8075	99.97	67.20	962
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-96-5P	99.95	72.80	2140
HSA-MIR-767-5P	99.95	70.85	993
HSA-MIR-128-3P	99.95	71.17	2484
HSA-MIR-216A-3P	99.95	71.19	2505
HSA-MIR-9983-3P	99.94	71.48	3631
HSA-MIR-3143	99.93	71.96	3104
HSA-MIR-205-3P	99.92	69.92	3165
HSA-MIR-12133	99.92	71.82	2006
HSA-MIR-1271-5P	99.91	71.99	1972
HSA-MIR-627-3P	99.90	71.42	3316
HSA-MIR-8087	99.90	69.55	1351
HSA-MIR-6783-3P	99.89	67.92	2059
HSA-MIR-3681-3P	99.88	70.46	2254
HSA-MIR-629-3P	99.85	67.99	1875

Literature-anchored findings (GeneRIF, showing 2)

• Single nucleotide polymorphisms in ZNF577 gene is associated with breast cancer. (PMID:21424380)
• ZNF577 Methylation Levels in Leukocytes From Women With Breast Cancer Is Modulated by Adiposity, Menopausal State, and the Mediterranean Diet. (PMID:32390948)

Cross-species orthologs

0 orthologs

Paralogs (176): ZNF195 (ENSG00000005801), ZNF112 (ENSG00000062370), ZNF275 (ENSG00000063587), ZNF37A (ENSG00000075407), ZNF510 (ENSG00000081386), ZNF506 (ENSG00000081665), ZNF268 (ENSG00000090612), MZF1 (ENSG00000099326), ZNF629 (ENSG00000102870), ZNF175 (ENSG00000105497), ZNF85 (ENSG00000105750), ZFP30 (ENSG00000120784), ZNF45 (ENSG00000124459), ZNF391 (ENSG00000124613), ZNF436 (ENSG00000125945), ZNF484 (ENSG00000127081), ZNF835 (ENSG00000127903), ZNF780B (ENSG00000128000), ZSCAN10 (ENSG00000130182), ZNF317 (ENSG00000130803), ZNF331 (ENSG00000130844), ZNF227 (ENSG00000131115), ZNF141 (ENSG00000131127), ZNF132 (ENSG00000131849), ZNF189 (ENSG00000136870), ZIM3 (ENSG00000141946), ZFP14 (ENSG00000142065), ZNF514 (ENSG00000144026), ZNF300 (ENSG00000145908), RBAK (ENSG00000146587), ZNF157 (ENSG00000147117), ZNF182 (ENSG00000147118), ZNF41 (ENSG00000147124), ZNF7 (ENSG00000147789), ZNF117 (ENSG00000152926), ZNF221 (ENSG00000159905), ZNF235 (ENSG00000159917), ZNF714 (ENSG00000160352), ZNF12 (ENSG00000164631), ZNF3 (ENSG00000166526)

Protein

Protein identifiers

Zinc finger protein 577 — Q9BSK1 (reviewed: Q9BSK1)

All UniProt accessions (8): A0A1W2PPU1, A0A1W2PPW0, A0A1W2PQ54, A0A1W2PRB6, Q9BSK1, A0A1W2PRW0, A0A1W2PRX5, A0A1W2PS72

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9BSK1-1	1	yes
Q9BSK1-2	2

RefSeq proteins (10): NP_001129062, NP_001357376, NP_001357377, NP_001357378, NP_001357379, NP_001357381, NP_001357384, NP_001357385, NP_001357386, NP_116068 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily

Pfam: PF00096, PF01352

UniProt features (21 total): sequence variant 8, zinc finger region 8, chain 1, domain 1, region of interest 1, splice variant 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BSK1-F1	64.28	0.32

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 40 (showing top): GOCC_NUCLEOLUS, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, WANG_RESPONSE_TO_GSK3_INHIBITOR_SB216763_UP, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, SETD7_TARGET_GENES, SUPT16H_TARGET_GENES, ZNF407_TARGET_GENES, ZSCAN30_TARGET_GENES, MIR580_5P, MIR4713_5P, MIR330_5P, MIR619_5P, MIR6513_3P, MIR4424, MIR4520_2_3P

GO Biological Process (4): regulation of transcription by RNA polymerase II (GO:0006357), regulation of DNA-templated transcription (GO:0006355), regulation of macromolecule metabolic process (GO:0060255), regulation of primary metabolic process (GO:0080090)

GO Molecular Function (6): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of metabolic process	2
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
macromolecule metabolic process	1
primary metabolic process	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

500 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF577	C12orf42	Q96LP6	505
ZNF577	COMMD2	Q86X83	457
ZNF577	ROPN1L	Q96C74	396
ZNF577	FAM219B	Q5XKK7	373
ZNF577	Q6GMV1	Q6GMV1	373
ZNF577	JHY	Q6NUN7	348
ZNF577	CMC2	Q9NRP2	328
ZNF577	FBXO41	Q8TF61	305
ZNF577	TXNDC16	Q9P2K2	300
ZNF577	TRIM58	Q8NG06	300
ZNF577	DENND4B	O75064	298
ZNF577	C9JR48	C9JR48	290
ZNF577	MCMDC2	Q4G0Z9	290
ZNF577	CFAP74	Q9C0B2	290
ZNF577	SIGLEC5	O15389	284

IntAct

5 interactions, top by confidence:

A	B	Type	Score
ZNF577	IPO7	psi-mi:“MI:0915”(physical association)	0.500
ZNF577	PCNA	psi-mi:“MI:0915”(physical association)	0.370
ZNF577	PPM1G	psi-mi:“MI:0914”(association)	0.350
ZNF577	DDI2	psi-mi:“MI:0914”(association)	0.350

BioGRID (6): LDHD (Affinity Capture-MS), CHAC2 (Affinity Capture-MS), MAPKAPK5 (Affinity Capture-MS), DDI2 (Affinity Capture-MS), ZNF577 (Affinity Capture-RNA), ZNF577 (Affinity Capture-Luminescence)

ESM2 similar proteins: A1KXM5, A1YFC1, A1YGK6, A2T7F2, A7KBS4, B2RXC5, E1JH25, G3X9G7, O96001, P16531, P18748, P34307, P88825, Q2EI21, Q3URU2, Q3V0C1, Q4V8E9, Q5JRM2, Q5R7U0, Q5RDG2, Q5RE50, Q5REF1, Q5SRN2, Q5SS00, Q5U4C1, Q6H236, Q6P1M9, Q6ZN11, Q810T2, Q8BUY8, Q8N3K9, Q8N660, Q8N720, Q8N859, Q8R2V3, Q920R4, Q921B4, Q923B3, Q95JY5, Q96D09

Diamond homologs: A0JPL0, A2VDP4, A6QLU5, A6QPT6, A8MQ14, A8MUZ8, A8MWA4, B1APH4, B2RXC5, E9PYI1, E9Q8G5, O75290, O94892, P08042, P0CH99, P0CI00, P17014, P17025, P17030, P17031, P17032, P17098, P21506, P51508, P51523, P51786, P51814, P52736, P52738, Q02525, Q03923, Q03936, Q06730, Q06732, Q0VCB0, Q13401, Q14587, Q16587, Q2M218, Q2M3X9

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

90 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	79
Likely benign	7
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1911 predictions. Top by Δscore:

Variant	Effect	Δscore
19:51817762:T:TA	acceptor_gain	1.0000
19:51817766:A:AG	acceptor_gain	1.0000
19:51817767:A:G	acceptor_gain	1.0000
19:51877376:CC:C	acceptor_gain	1.0000
19:51877377:CC:C	acceptor_gain	1.0000
19:51878383:CCTTA:C	donor_loss	1.0000
19:51878384:CTTA:C	donor_loss	1.0000
19:51878385:TTACC:T	donor_loss	1.0000
19:51878386:TA:T	donor_loss	1.0000
19:51878387:A:AG	donor_loss	1.0000
19:51878388:C:CA	donor_loss	1.0000
19:51877278:TTA:T	donor_loss	0.9900
19:51877279:TA:T	donor_loss	0.9900
19:51877280:A:AC	donor_gain	0.9900
19:51877280:A:C	donor_loss	0.9900
19:51877281:C:CC	donor_gain	0.9900
19:51877374:TACC:T	acceptor_gain	0.9900
19:51877378:C:CC	acceptor_gain	0.9900
19:51877378:CTGTA:C	acceptor_loss	0.9900
19:51877379:T:A	acceptor_loss	0.9900
19:51878387:A:AC	donor_gain	0.9900
19:51878388:C:CC	donor_gain	0.9900
19:51878427:T:TA	donor_gain	0.9900
19:51878513:CCC:C	acceptor_gain	0.9900
19:51878514:CCC:C	acceptor_gain	0.9900
19:51879718:A:AC	donor_gain	0.9900
19:51880325:C:A	donor_gain	0.9900
19:51881035:G:GT	donor_gain	0.9900
19:51881058:A:T	donor_gain	0.9900
19:51887795:C:CA	donor_gain	0.9900

AlphaMissense

3144 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:51872901:A:C	F363L	0.999
19:51872901:A:T	F363L	0.999
19:51872903:A:G	F363L	0.999
19:51872953:C:G	R346P	0.998
19:51872985:A:C	F335L	0.998
19:51872985:A:T	F335L	0.998
19:51872987:A:G	F335L	0.998
19:51873069:G:C	F307L	0.997
19:51873069:G:T	F307L	0.997
19:51873071:A:G	F307L	0.997
19:51873237:G:C	F251L	0.997
19:51873237:G:T	F251L	0.997
19:51873239:A:G	F251L	0.997
19:51873321:G:C	F223L	0.997
19:51873321:G:T	F223L	0.997
19:51873323:A:G	F223L	0.997
19:51872956:T:G	Q345P	0.996
19:51872958:A:C	H344Q	0.996
19:51872958:A:T	H344Q	0.996
19:51873052:A:G	L313P	0.996
19:51873153:A:C	F279L	0.996
19:51873153:A:T	F279L	0.996
19:51873155:A:G	F279L	0.996
19:51872960:G:C	H344D	0.995
19:51872884:A:G	L369P	0.994
19:51872946:G:C	H348Q	0.994
19:51872946:G:T	H348Q	0.994
19:51872968:A:G	L341P	0.994
19:51873042:A:C	H316Q	0.994
19:51873042:A:T	H316Q	0.994

dbSNP variants (sampled 300 via entrez): RS1000019335 (19:51869944 C>A,T), RS1000029854 (19:51814204 T>C), RS1000051189 (19:51875934 T>C), RS1000062805 (19:51863593 A>C), RS1000101388 (19:51875678 G>A), RS1000200434 (19:51847472 C>G), RS1000211444 (19:51840881 G>A,C), RS1000233164 (19:51858754 G>C), RS1000235178 (19:51868952 A>C), RS1000302356 (19:51846596 T>C), RS1000325392 (19:51820648 G>A,C), RS1000361604 (19:51852758 T>G), RS1000437183 (19:51874833 A>G), RS1000437756 (19:51834316 T>C), RS1000456759 (19:51881359 A>G)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST001012_2	Breast cancer	7.000000e-06
GCST004866_7	Alopecia areata	6.000000e-06
GCST005760_1	Dimensional psychopathology (Cognitive)	3.000000e-08
GCST007277_24	Tourette syndrome	9.000000e-07

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0009098	cognitive domain measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

10 total (human), top 10 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression	2
arsenite	increases methylation	1
CGP 52608	affects binding, increases reaction	1
Resveratrol	affects cotreatment, increases expression	1
Leflunomide	increases expression	1
Benzo(a)pyrene	affects methylation, increases methylation	1
Dichlorodiphenyl Dichloroethylene	decreases expression	1
Plant Extracts	affects cotreatment, increases expression	1
Cyclosporine	decreases expression	1
Aflatoxin B1	increases methylation	1

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_XW66	HEK293 eGFP-ZNF577	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.