ZNF677

Summary

ZNF677 (zinc finger protein 677, HGNC:28730) is a protein-coding gene on chromosome 19q13.42, encoding Zinc finger protein 677 (Q86XU0). May be involved in transcriptional regulation.

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.

Source: NCBI Gene 342926 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 97 total
• MANE Select transcript: NM_182609

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28730
Approved symbol	ZNF677
Name	zinc finger protein 677
Location	19q13.42
Locus type	gene with protein product
Status	Approved
Aliases	MGC48625
Ensembl gene	ENSG00000197928
Ensembl biotype	protein_coding
Entrez	342926

Gene structure

Transcript identifiers

Ensembl transcripts: 12 — 9 protein_coding, 2 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000333952, ENST00000593539, ENST00000594517, ENST00000594681, ENST00000595293, ENST00000598513, ENST00000598806, ENST00000599012, ENST00000599328, ENST00000601413, ENST00000601828, ENST00000881288

RefSeq mRNA: 13 — MANE Select: NM_182609 NM_001317998, NM_001385608, NM_001385609, NM_001385610, NM_001385611, NM_001385612, NM_001385613, NM_001385614, NM_001385615, NM_001385616, NM_001385617, NM_001385618, NM_182609

CCDS: CCDS12861, CCDS92680

Canonical transcript exons

ENST00000598513 — 5 exons

Exon	Start	End
ENSE00001426495	53243744	53243897
ENSE00001509701	53253086	53253156
ENSE00003054538	53254834	53254873
ENSE00003216061	53235381	53238557
ENSE00003554092	53251536	53251605

Expression profiles

Bgee: expression breadth ubiquitous, 244 present calls, max score 97.45.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 6.2662 / max 132.9588, expressed in 1413 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
182557	3.1777	1258
182556	2.9266	1202
182558	0.1619	44

Top tissues by expression

250 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	97.45	gold quality
oocyte	CL:0000023	96.45	gold quality
ganglionic eminence	UBERON:0004023	94.91	gold quality
left testis	UBERON:0004533	94.56	gold quality
right testis	UBERON:0004534	94.38	gold quality
epithelial cell of pancreas	CL:0000083	93.98	gold quality
ventricular zone	UBERON:0003053	93.23	gold quality
testis	UBERON:0000473	93.18	gold quality
endothelial cell	CL:0000115	92.69	gold quality
oviduct epithelium	UBERON:0004804	92.55	gold quality
cortical plate	UBERON:0005343	91.94	gold quality
calcaneal tendon	UBERON:0003701	89.72	gold quality
sperm	CL:0000019	89.43	gold quality
amniotic fluid	UBERON:0000173	88.69	gold quality
germinal epithelium of ovary	UBERON:0001304	88.59	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	87.23	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	87.10	gold quality
tibia	UBERON:0000979	86.80	gold quality
adrenal tissue	UBERON:0018303	86.25	gold quality
ovary	UBERON:0000992	85.85	gold quality
fallopian tube	UBERON:0003889	85.70	gold quality
smooth muscle tissue	UBERON:0001135	85.64	gold quality
left ovary	UBERON:0002119	85.54	gold quality
parietal pleura	UBERON:0002400	84.80	gold quality
right atrium auricular region	UBERON:0006631	84.23	gold quality
body of uterus	UBERON:0009853	84.21	gold quality
right uterine tube	UBERON:0001302	84.18	gold quality
right ovary	UBERON:0002118	84.15	gold quality
islet of Langerhans	UBERON:0000006	83.74	gold quality
cardiac atrium	UBERON:0002081	83.47	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-CURD-89	no	51.44
E-MTAB-6678	no	3.13
E-ANND-3	no	0.00

Regulation

Is transcription factor: yes

JASPAR motifs

Motif	Name	Family
MA2101.1	ZNF677	More than 3 adjacent zinc fingers

JASPAR matrix evidence (PMIDs): PMID:27852650

miRNA regulators (miRDB)

76 targeting ZNF677, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-6873-3P	100.00	71.42	2626
HSA-MIR-3064-3P	100.00	70.09	1254
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-1252-5P	100.00	69.80	2774
HSA-MIR-4768-5P	100.00	69.49	2861
HSA-MIR-6833-3P	100.00	70.63	3197
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-433-3P	99.98	69.37	1203
HSA-MIR-499A-5P	99.98	70.79	1323
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-6845-3P	99.94	66.88	1439
HSA-MIR-141-3P	99.94	72.79	2421
HSA-MIR-200A-3P	99.94	72.68	2420
HSA-MIR-539-5P	99.93	70.30	2855
HSA-MIR-515-5P	99.92	69.82	2343
HSA-MIR-519E-5P	99.92	69.62	2358
HSA-MIR-589-3P	99.91	69.62	2088
HSA-MIR-10395-5P	99.86	67.35	676
HSA-MIR-4799-5P	99.82	70.60	2663
HSA-MIR-6515-3P	99.82	68.19	1933
HSA-MIR-139-5P	99.80	69.50	1399
HSA-MIR-448	99.79	72.37	2103
HSA-MIR-4679	99.76	69.19	1229
HSA-MIR-4517	99.76	69.19	1867
HSA-MIR-3617-5P	99.75	69.41	1968
HSA-MIR-641	99.75	69.35	1975
HSA-MIR-4422	99.72	72.07	2908

Literature-anchored findings (GeneRIF, showing 8)

• our results demonstrate that ZNF677 is trancriptionally regulated by methylation in non-small cell lung cancers and suggest that ZNF677 has tumor cell growth suppressing properties (PMID:25504438)
• ZNF677 functions as a tumor suppressor and is frequently silenced via promoter methylation in thyroid cancer (PMID:29997231)
• ZNF677 downregulation by promoter hypermethylation as a driver event through gastric tumorigenesis. (PMID:34171355)
• Loss of ZNF677 Expression Is an Independent Predictor for Distant Metastasis in Middle Eastern Papillary Thyroid Carcinoma Patients. (PMID:34360599)
• Loss of ZNF677 expression is a predictive biomarker for lymph node metastasis in Middle Eastern Colorectal Cancer. (PMID:34785764)
• Overexpression of zinc-finger protein 677 inhibits proliferation and invasion by and induces apoptosis in clear cell renal cell carcinoma. (PMID:35164660)
• ZNF677 suppresses renal cell carcinoma progression through N6-methyladenosine and transcriptional repression of CDKN3. (PMID:35678231)
• ZNF677 inhibits oral squamous cell carcinoma growth and tumor stemness by regulating FOXO3a. (PMID:37129799)

Cross-species orthologs

0 orthologs

Paralogs (176): ZNF195 (ENSG00000005801), ZNF112 (ENSG00000062370), ZNF275 (ENSG00000063587), ZNF37A (ENSG00000075407), ZNF510 (ENSG00000081386), ZNF506 (ENSG00000081665), ZNF268 (ENSG00000090612), MZF1 (ENSG00000099326), ZNF629 (ENSG00000102870), ZNF175 (ENSG00000105497), ZNF85 (ENSG00000105750), ZFP30 (ENSG00000120784), ZNF45 (ENSG00000124459), ZNF391 (ENSG00000124613), ZNF436 (ENSG00000125945), ZNF484 (ENSG00000127081), ZNF835 (ENSG00000127903), ZNF780B (ENSG00000128000), ZSCAN10 (ENSG00000130182), ZNF317 (ENSG00000130803), ZNF331 (ENSG00000130844), ZNF227 (ENSG00000131115), ZNF141 (ENSG00000131127), ZNF132 (ENSG00000131849), ZNF189 (ENSG00000136870), ZIM3 (ENSG00000141946), ZFP14 (ENSG00000142065), ZNF514 (ENSG00000144026), ZNF300 (ENSG00000145908), RBAK (ENSG00000146587), ZNF157 (ENSG00000147117), ZNF182 (ENSG00000147118), ZNF41 (ENSG00000147124), ZNF7 (ENSG00000147789), ZNF117 (ENSG00000152926), ZNF221 (ENSG00000159905), ZNF235 (ENSG00000159917), ZNF714 (ENSG00000160352), ZNF577 (ENSG00000161551), ZNF12 (ENSG00000164631)

Protein

Protein identifiers

Zinc finger protein 677 — Q86XU0 (reviewed: Q86XU0)

All UniProt accessions (6): Q86XU0, M0QYA4, M0R079, M0R0I7, M0R1V2, M0R297

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (13): NP_001304927, NP_001372537, NP_001372538, NP_001372539, NP_001372540, NP_001372541, NP_001372542, NP_001372543, NP_001372544, NP_001372545, NP_001372546, NP_001372547, NP_872415* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050826	Krueppel_C2H2_ZnFinger	Family

Pfam: PF00096, PF01352

UniProt features (14 total): zinc finger region 10, sequence variant 2, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q86XU0-F1	60.12	0.27

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 50 (showing top): GSE45365_CD8A_DC_VS_CD11B_DC_IFNAR_KO_UP, WEBER_METHYLATED_IN_COLON_CANCER, VECCHI_GASTRIC_CANCER_EARLY_DN, OSMAN_BLADDER_CANCER_DN, LEE_RECENT_THYMIC_EMIGRANT, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, DODD_NASOPHARYNGEAL_CARCINOMA_DN, ZWANG_TRANSIENTLY_UP_BY_2ND_EGF_PULSE_ONLY, GCM_RAB10, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, ALKBH3_TARGET_GENES, ZNF22_TARGET_GENES, MIR4422, MIR5692B_MIR5692C, MIR539_5P

GO Biological Process (2): regulation of transcription by RNA polymerase II (GO:0006357), regulation of DNA-templated transcription (GO:0006355)

GO Molecular Function (5): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

478 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF677	TP53BP1	Q12888	490
ZNF677	OR6P1	Q8NGX9	447
ZNF677	VN1R4	Q7Z5H5	418
ZNF677	VN1R2	Q8NFZ6	392
ZNF677	CCDC74B	Q96LY2	372
ZNF677	ZNF385D	Q9H6B1	370
ZNF677	CCDC169	A6NNP5	358
ZNF677	CCDC190	Q86UF4	348
ZNF677	ARHGAP40	Q5TG30	348
ZNF677	CCDC81	Q6ZN84	327
ZNF677	CCDC125	Q86Z20	324
ZNF677	CXorf38	Q8TB03	320
ZNF677	TMEM121	Q9BTD3	318
ZNF677	Q8WV35	Q8WV35	318
ZNF677	JPH4	Q96JJ6	318

IntAct

5 interactions, top by confidence:

A	B	Type	Score
ZNF677	WFDC1	psi-mi:“MI:0915”(physical association)	0.370
ZNF677	PARVG	psi-mi:“MI:0915”(physical association)	0.370
ZNF765	TRIM24	psi-mi:“MI:0914”(association)	0.350
ZNF677	ZNF320	psi-mi:“MI:0914”(association)	0.350

BioGRID (26): ZNF195 (Affinity Capture-MS), ZNF320 (Affinity Capture-MS), SFSWAP (Affinity Capture-MS), DDX52 (Affinity Capture-MS), SCAF11 (Affinity Capture-MS), SAFB2 (Affinity Capture-MS), ZNF430 (Affinity Capture-MS), MAP9 (Affinity Capture-MS), FOCAD (Affinity Capture-MS), ZC3H4 (Affinity Capture-MS), DCAF13 (Affinity Capture-MS), TRIM37 (Affinity Capture-MS), CENPB (Affinity Capture-MS), KIAA1429 (Affinity Capture-MS), PHF3 (Affinity Capture-MS)

ESM2 similar proteins: A0A1W2PQL4, A0JPL0, A2RRD8, A6NHJ4, A6NP11, B4DX44, O75346, O95780, P0CB33, P21506, P51508, P52738, Q0D2J5, Q0VGE8, Q12901, Q13360, Q14593, Q15973, Q2M218, Q2M3X9, Q2VY69, Q3SXZ3, Q5HY98, Q5RCJ2, Q5VIY5, Q6J6I6, Q7L2R6, Q86XU0, Q86Y25, Q8IW36, Q8IYN0, Q8IYX0, Q8N782, Q8N883, Q8N9Z0, Q8NDP4, Q95K49, Q969W8, Q96H40, Q96N58

Diamond homologs: A0A1W2PQL4, A2RRD8, A2VDQ7, A6NHJ4, A6NK75, A6NN14, A6NNF4, A6NP11, B4DX44, B4DXR9, E9PW05, O14628, O43345, O75290, O75346, O75373, O75820, P0CB33, P0CJ79, P0DKX0, P10755, P17032, P17035, P52738, Q02386, Q03923, Q03936, Q05481, Q08AN1, Q09FC8, Q0VGE8, Q14586, Q14593, Q147U1, Q15928, Q3MIS6, Q3SXZ3, Q494X3, Q4R6C2, Q5HY98

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

97 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	85
Likely benign	8
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1167 predictions. Top by Δscore:

Variant	Effect	Δscore
19:53239109:ACAC:A	donor_gain	1.0000
19:53239110:CACC:C	donor_gain	1.0000
19:53243743:CCAT:C	donor_gain	1.0000
19:53251602:CAGT:C	acceptor_gain	1.0000
19:53251606:C:CC	acceptor_gain	1.0000
19:53253081:TTTA:T	donor_loss	1.0000
19:53253082:TTA:T	donor_loss	1.0000
19:53253083:TAC:T	donor_loss	1.0000
19:53253084:A:AC	donor_gain	1.0000
19:53253084:A:AT	donor_loss	1.0000
19:53253085:C:CC	donor_gain	1.0000
19:53253085:CCATT:C	donor_gain	1.0000
19:53253152:GTGGG:G	acceptor_gain	1.0000
19:53253153:TGGG:T	acceptor_gain	1.0000
19:53253154:GGG:G	acceptor_gain	1.0000
19:53253155:GG:G	acceptor_gain	1.0000
19:53253157:C:CC	acceptor_gain	1.0000
19:53253157:CTGCA:C	acceptor_loss	1.0000
19:53253160:CAG:C	acceptor_gain	1.0000
19:53253161:A:T	acceptor_gain	1.0000
19:53253162:G:C	acceptor_gain	1.0000
19:53253162:G:GC	acceptor_gain	1.0000
19:53238555:TAT:T	acceptor_gain	0.9900
19:53239110:CA:C	donor_gain	0.9900
19:53243739:GTTA:G	donor_loss	0.9900
19:53243740:TTACC:T	donor_loss	0.9900
19:53243742:AC:A	donor_loss	0.9900
19:53243743:C:CA	donor_loss	0.9900
19:53243763:AT:A	donor_gain	0.9900
19:53243764:T:TA	donor_gain	0.9900

AlphaMissense

3940 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:53237239:A:C	F496L	0.997
19:53237239:A:T	F496L	0.997
19:53237241:A:G	F496L	0.997
19:53237323:A:C	F468L	0.997
19:53237323:A:T	F468L	0.997
19:53237325:A:G	F468L	0.997
19:53237575:A:C	F384L	0.996
19:53237575:A:T	F384L	0.996
19:53237577:A:G	F384L	0.996
19:53237155:A:C	F524L	0.995
19:53237155:A:T	F524L	0.995
19:53237157:A:G	F524L	0.995
19:53237659:A:C	F356L	0.995
19:53237659:A:T	F356L	0.995
19:53237661:A:G	F356L	0.995
19:53237222:A:G	L502P	0.993
19:53237407:A:C	F440L	0.993
19:53237407:A:T	F440L	0.993
19:53237409:A:G	F440L	0.993
19:53237827:A:C	F300L	0.993
19:53237827:A:T	F300L	0.993
19:53237829:A:G	F300L	0.993
19:53237306:A:G	L474P	0.991
19:53237380:A:C	H449Q	0.990
19:53237380:A:T	H449Q	0.990
19:53237491:A:C	F412L	0.990
19:53237491:A:T	F412L	0.990
19:53237493:A:G	F412L	0.990
19:53237911:A:C	F272L	0.990
19:53237911:A:T	F272L	0.990

dbSNP variants (sampled 300 via entrez): RS1000062047 (19:53246575 C>A,T), RS1000125975 (19:53242432 G>A,C), RS1000263130 (19:53242431 A>T), RS1000367625 (19:53247739 G>C), RS1000753206 (19:53236530 T>G), RS1001162532 (19:53254160 T>C), RS1001282937 (19:53253870 G>A,C), RS1001336356 (19:53250795 C>G,T), RS1001382439 (19:53237599 T>C,G), RS1001573076 (19:53254491 G>A,T), RS1001925049 (19:53255749 T>C), RS1002073160 (19:53249226 G>T), RS1002209737 (19:53248782 A>C,G,T), RS1002331063 (19:53254454 C>T), RS1002367944 (19:53250186 T>C)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

16 total (human), top 16 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	decreases expression, decreases methylation	3
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
arsenite	decreases expression, increases methylation	1
potassium chromate(VI)	increases expression	1
Sunitinib	increases expression	1
Air Pollutants	decreases expression, increases abundance	1
Arsenic	affects methylation	1
Benzo(a)pyrene	increases methylation	1
Doxorubicin	decreases expression	1
Nickel	decreases expression	1
Phenylmercuric Acetate	decreases expression	1
Tetrachlorodibenzodioxin	affects expression	1
Tobacco Smoke Pollution	increases methylation	1
Vanadates	decreases expression	1
Particulate Matter	decreases expression, increases abundance	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.