ZNF71

Summary

ZNF71 (zinc finger protein 71, HGNC:13141) is a protein-coding gene on chromosome 19q13.4, encoding Endothelial zinc finger protein induced by tumor necrosis factor alpha (Q9NQZ8). May be involved in transcriptional regulation.

Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.

Source: NCBI Gene 58491 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 77 total
• MANE Select transcript: NM_001370215

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:13141
Approved symbol	ZNF71
Name	zinc finger protein 71
Location	19q13.4
Locus type	gene with protein product
Status	Approved
Aliases	Cos26, EZFIT
Ensembl gene	ENSG00000197951
Ensembl biotype	protein_coding
OMIM	194545
Entrez	58491

Gene structure

Transcript identifiers

Ensembl transcripts: 7 — 6 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000328070, ENST00000594944, ENST00000599599, ENST00000716547, ENST00000716548, ENST00000716549, ENST00000862326

RefSeq mRNA: 3 — MANE Select: NM_001370215 NM_001370214, NM_001370215, NM_021216

CCDS: CCDS12947, CCDS92695

Canonical transcript exons

ENST00000599599 — 4 exons

Exon	Start	End
ENSE00003022213	56613812	56613938
ENSE00003076760	56621268	56624486
ENSE00003834959	56601507	56601591
ENSE00004030077	56595302	56595428

Expression profiles

Bgee: expression breadth ubiquitous, 179 present calls, max score 83.28.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 6.0921 / max 65.5286, expressed in 1566 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
177730	6.0921	1566
177729	1.2955	871

Top tissues by expression

250 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	83.28	gold quality
ganglionic eminence	UBERON:0004023	80.13	gold quality
cortical plate	UBERON:0005343	79.92	gold quality
tibialis anterior	UBERON:0001385	77.71	silver quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	77.44	gold quality
stromal cell of endometrium	CL:0002255	77.36	gold quality
ventricular zone	UBERON:0003053	76.36	gold quality
granulocyte	CL:0000094	74.24	gold quality
apex of heart	UBERON:0002098	74.22	gold quality
secondary oocyte	CL:0000655	74.18	gold quality
kidney epithelium	UBERON:0004819	73.72	gold quality
lower esophagus muscularis layer	UBERON:0035833	73.30	gold quality
lower esophagus	UBERON:0013473	73.28	gold quality
gastrocnemius	UBERON:0001388	73.24	gold quality
muscle of leg	UBERON:0001383	73.19	gold quality
popliteal artery	UBERON:0002250	73.04	gold quality
tibial artery	UBERON:0007610	73.02	gold quality
prefrontal cortex	UBERON:0000451	73.00	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	72.44	gold quality
buccal mucosa cell	CL:0002336	72.39	gold quality
aorta	UBERON:0000947	72.32	gold quality
body of uterus	UBERON:0009853	72.27	gold quality
heart left ventricle	UBERON:0002084	71.85	gold quality
muscle layer of sigmoid colon	UBERON:0035805	71.73	gold quality
right coronary artery	UBERON:0001625	71.67	gold quality
thoracic aorta	UBERON:0001515	71.56	gold quality
endothelial cell	CL:0000115	71.43	gold quality
hindlimb stylopod muscle	UBERON:0004252	71.43	gold quality
ascending aorta	UBERON:0001496	71.40	gold quality
cardiac ventricle	UBERON:0002082	71.39	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	3.25

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

64 targeting ZNF71, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-511-3P	99.99	68.85	1467
HSA-MIR-4482-3P	99.98	72.50	3147
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-9902	99.89	69.15	2250
HSA-MIR-6780A-5P	99.88	66.69	2776
HSA-MIR-12119	99.87	68.35	1653
HSA-MIR-1273H-5P	99.77	66.32	2471
HSA-MIR-30B-3P	99.70	65.76	2325
HSA-MIR-3689A-3P	99.70	65.73	2306
HSA-MIR-3689B-3P	99.70	65.71	2311
HSA-MIR-3689C	99.70	65.71	2311
HSA-MIR-6779-5P	99.70	65.76	2363
HSA-MIR-379-3P	99.69	69.60	1524
HSA-MIR-411-3P	99.69	69.63	1524
HSA-MIR-6887-3P	99.66	67.83	1778
HSA-MIR-3202	99.66	67.70	2737
HSA-MIR-3177-5P	99.65	70.38	1174
HSA-MIR-452-5P	99.65	69.63	1762
HSA-MIR-4676-3P	99.65	69.31	1733
HSA-MIR-892C-3P	99.65	69.38	1745
HSA-MIR-1249-5P	99.61	66.55	2049
HSA-MIR-6797-5P	99.61	66.55	2084
HSA-MIR-24-3P	99.59	69.97	1934
HSA-MIR-4649-3P	99.56	66.90	1783
HSA-MIR-3171	99.49	69.06	776
HSA-MIR-4441	99.49	66.56	3216
HSA-MIR-6871-3P	99.43	68.85	741
HSA-MIR-330-3P	99.41	69.95	2521
HSA-MIR-3911	99.38	66.95	1087
HSA-MIR-5690	99.25	67.58	1012

Literature-anchored findings (GeneRIF, showing 1)

• Molecular Analysis of ZNF71 KRAB in Non-Small-Cell Lung Cancer. (PMID:33916522)

Cross-species orthologs

0 orthologs

Paralogs (176): ZNF195 (ENSG00000005801), ZNF112 (ENSG00000062370), ZNF275 (ENSG00000063587), ZNF37A (ENSG00000075407), ZNF510 (ENSG00000081386), ZNF506 (ENSG00000081665), ZNF268 (ENSG00000090612), MZF1 (ENSG00000099326), ZNF629 (ENSG00000102870), ZNF175 (ENSG00000105497), ZNF85 (ENSG00000105750), ZFP30 (ENSG00000120784), ZNF45 (ENSG00000124459), ZNF391 (ENSG00000124613), ZNF436 (ENSG00000125945), ZNF484 (ENSG00000127081), ZNF835 (ENSG00000127903), ZNF780B (ENSG00000128000), ZSCAN10 (ENSG00000130182), ZNF317 (ENSG00000130803), ZNF331 (ENSG00000130844), ZNF227 (ENSG00000131115), ZNF141 (ENSG00000131127), ZNF132 (ENSG00000131849), ZNF189 (ENSG00000136870), ZIM3 (ENSG00000141946), ZFP14 (ENSG00000142065), ZNF514 (ENSG00000144026), ZNF300 (ENSG00000145908), RBAK (ENSG00000146587), ZNF157 (ENSG00000147117), ZNF182 (ENSG00000147118), ZNF41 (ENSG00000147124), ZNF7 (ENSG00000147789), ZNF117 (ENSG00000152926), ZNF221 (ENSG00000159905), ZNF235 (ENSG00000159917), ZNF714 (ENSG00000160352), ZNF577 (ENSG00000161551), ZNF12 (ENSG00000164631)

Protein

Protein identifiers

Endothelial zinc finger protein induced by tumor necrosis factor alpha — Q9NQZ8 (reviewed: Q9NQZ8)

Alternative names: Zinc finger protein 71

All UniProt accessions (2): Q9NQZ8, M0R0C0

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Tissue specificity. Highly expressed in placenta, followed by brain, testis, pancreas, heart, small intestine, muscle, uterus, prostate and peripheral blood leukocytes. Not detected in liver, lung, colon, stomach, salivary and thyroid gland.

Induction. By TNF.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (3): NP_001357143, NP_001357144, NP_067039 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR013087	Znf_C2H2_type	Domain
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050752	C2H2-ZF_domain	Family

Pfam: PF00096

UniProt features (20 total): zinc finger region 13, sequence variant 3, region of interest 2, chain 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9NQZ8-F1	77.36	0.61

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 32 (showing top): GSE45365_CD8A_DC_VS_CD11B_DC_IFNAR_KO_MCMV_INFECTION_UP, BASAKI_YBX1_TARGETS_UP, GRYDER_PAX3FOXO1_ENHANCERS_IN_TADS, SENESE_HDAC3_TARGETS_DN, NUYTTEN_NIPP1_TARGETS_DN, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, HORIUCHI_WTAP_TARGETS_UP, GOBP_POSITIVE_REGULATION_OF_TRANSCRIPTION_BY_RNA_POLYMERASE_II, GOMF_DNA_BINDING_TRANSCRIPTION_ACTIVATOR_ACTIVITY, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, ZNF350_TARGET_GENES, ZNF92_TARGET_GENES, MIR4742_3P, MIR146A_3P, MIR3926

GO Biological Process (3): regulation of transcription by RNA polymerase II (GO:0006357), regulation of DNA-templated transcription (GO:0006355), positive regulation of transcription by RNA polymerase II (GO:0045944)

GO Molecular Function (6): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228), zinc ion binding (GO:0008270), DNA binding (GO:0003677), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
transcription by RNA polymerase II	2
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
regulation of DNA-templated transcription	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
regulation of transcription by RNA polymerase II	1
positive regulation of DNA-templated transcription	1
cis-regulatory region sequence-specific DNA binding	1
DNA-binding transcription factor activity, RNA polymerase II-specific	1
DNA-binding transcription activator activity	1
positive regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

408 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF71	C9JR48	C9JR48	895
ZNF71	GCSAML	Q5JQS6	429
ZNF71	MTMR8	Q96EF0	428
ZNF71	ALB	P02768	384
ZNF71	CLVS1	Q8IUQ0	383
ZNF71	CCDC71L	Q8N9Z2	383
ZNF71	LONRF3	Q496Y0	360
ZNF71	K7EJK4	K7EJK4	323
ZNF71	MAST4	O15021	323
ZNF71	CSTPP1	Q9H6J7	322
ZNF71	FUT7	Q11130	321
ZNF71	EEPD1	Q7L9B9	318
ZNF71	SHISA6	Q6ZSJ9	314
ZNF71	ZNF438	Q7Z4V0	312
ZNF71	ASCC3	Q8N3C0	297

IntAct

84 interactions, top by confidence:

A	B	Type	Score
ZBTB24	ZNF71	psi-mi:“MI:0915”(physical association)	0.740
ZNF71	MAP3K20	psi-mi:“MI:0915”(physical association)	0.670
U2AF2	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF648	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF71	PICK1	psi-mi:“MI:0915”(physical association)	0.560
ZBTB9	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
DVL3	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
FXR1	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
TRIM41	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF497	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
PGRMC1	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
PHF1	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF792	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
MFAP1	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZGPAT	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
SART3	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
LGALS14	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF71	ZNF835	psi-mi:“MI:0915”(physical association)	0.560
PRPF31	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF417	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF524	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
BYSL	ZNF71	psi-mi:“MI:0915”(physical association)	0.560
ZNF71	NVL	psi-mi:“MI:0914”(association)	0.530
ZNF71	DKC1	psi-mi:“MI:0914”(association)	0.530

BioGRID (107): ZNF71 (Two-hybrid), ZNF71 (Two-hybrid), ZFP2 (Affinity Capture-MS), HIST1H1A (Affinity Capture-MS), HIST1H1T (Affinity Capture-MS), ZNF75A (Affinity Capture-MS), RPS27A (Affinity Capture-MS), ZNF7 (Affinity Capture-MS), RPL36AL (Affinity Capture-MS), ZNF354A (Affinity Capture-MS), KIAA0020 (Affinity Capture-MS), CENPI (Affinity Capture-MS), RRP8 (Affinity Capture-MS), FBXO11 (Affinity Capture-MS), CENPN (Affinity Capture-MS)

ESM2 similar proteins: C9JN71, O75290, O75820, P08043, P0CJ79, P10076, P15620, P17017, P17021, P17027, P17039, P18714, P51523, Q08AN1, Q14587, Q3KP31, Q3ZCX4, Q4R4C7, Q52M93, Q5MCW4, Q5R5Q6, Q5R5U3, Q5R5Y7, Q5R9F0, Q5RB30, Q5RDX1, Q5REA0, Q5REI6, Q6P3V2, Q6ZN19, Q6ZN57, Q6ZNA1, Q7Z3V5, Q80W31, Q86UE3, Q86YE8, Q8BPP0, Q8C827, Q8N4W9, Q8NB50

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

77 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	73
Likely benign	2
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

523 predictions. Top by Δscore:

Variant	Effect	Δscore
19:56601505:A:AG	acceptor_gain	0.9900
19:56601506:G:GG	acceptor_gain	0.9900
19:56601506:GCACT:G	acceptor_gain	0.9900
19:56610073:C:T	donor_gain	0.9900
19:56613899:G:T	donor_gain	0.9900
19:56613922:G:GT	donor_gain	0.9900
19:56613936:TGGG:T	donor_loss	0.9900
19:56613937:GG:G	donor_gain	0.9900
19:56613938:GG:G	donor_gain	0.9900
19:56613938:GGTA:G	donor_loss	0.9900
19:56613939:G:GG	donor_gain	0.9900
19:56613939:GT:G	donor_loss	0.9900
19:56613940:T:C	donor_loss	0.9900
19:56621263:TTCA:T	acceptor_loss	0.9900
19:56621266:A:AC	acceptor_loss	0.9900
19:56621266:A:AG	acceptor_gain	0.9900
19:56621267:G:GG	acceptor_gain	0.9900
19:56621267:GACT:G	acceptor_gain	0.9900
19:56601506:GCA:G	acceptor_gain	0.9800
19:56601589:CTGG:C	donor_loss	0.9800
19:56601590:TGGT:T	donor_loss	0.9800
19:56601592:G:GC	donor_loss	0.9800
19:56601592:G:GG	donor_gain	0.9800
19:56601593:T:TT	donor_loss	0.9800
19:56601594:G:GA	donor_loss	0.9800
19:56601595:AGT:A	donor_loss	0.9800
19:56601596:G:C	donor_loss	0.9800
19:56613941:AAGGG:A	donor_loss	0.9800
19:56621266:AGACT:A	acceptor_gain	0.9800
19:56621267:GA:G	acceptor_gain	0.9800

AlphaMissense

3636 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:56621870:T:C	F195L	0.999
19:56621872:C:A	F195L	0.999
19:56621872:C:G	F195L	0.999
19:56621954:T:C	F223L	0.999
19:56621956:C:A	F223L	0.999
19:56621956:C:G	F223L	0.999
19:56622038:T:C	F251L	0.999
19:56622040:C:A	F251L	0.999
19:56622040:C:G	F251L	0.999
19:56622072:G:C	R262P	0.999
19:56622122:T:C	F279L	0.999
19:56622124:C:A	F279L	0.999
19:56622124:C:G	F279L	0.999
19:56622206:T:C	F307L	0.999
19:56622208:C:A	F307L	0.999
19:56622208:C:G	F307L	0.999
19:56622290:T:C	F335L	0.999
19:56622292:C:A	F335L	0.999
19:56622292:C:G	F335L	0.999
19:56622374:T:C	F363L	0.999
19:56622376:C:A	F363L	0.999
19:56622376:C:G	F363L	0.999
19:56622542:T:C	F419L	0.999
19:56622544:C:A	F419L	0.999
19:56622544:C:G	F419L	0.999
19:56622626:T:C	F447L	0.999
19:56622628:C:A	F447L	0.999
19:56622628:C:G	F447L	0.999
19:56622645:T:C	L453P	0.999
19:56622710:T:C	F475L	0.999

dbSNP variants (sampled 300 via entrez): RS1000170376 (19:56611516 A>G), RS1000192778 (19:56603239 C>T), RS1000251394 (19:56603999 C>T), RS1000263698 (19:56611777 G>C), RS1000329047 (19:56610085 C>G), RS1000381277 (19:56610232 A>G), RS1000544451 (19:56617794 G>C), RS1000598922 (19:56593396 A>T), RS1000806896 (19:56605515 A>G), RS1000845335 (19:56593690 G>A), RS1000934597 (19:56622184 C>G), RS1001018853 (19:56616621 G>C), RS1001360652 (19:56603008 A>G), RS1001512187 (19:56616970 C>T), RS1001541884 (19:56609748 G>A)

Disease associations

OMIM: gene MIM:194545 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST002122_2	IgE levels in asthmatics	8.000000e-06
GCST004068_26	Venous thromboembolism adjusted for sickle cell variant rs77121243-T	3.000000e-06
GCST008876_7	Non-lobar intracerebral hemorrhage (MTAG)	7.000000e-06
GCST90002388_383	Lymphocyte count	7.000000e-10

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0010178	non-lobar intracerebral hemorrhage
EFO:0004587	lymphocyte count

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

20 total (human), top 20 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
FR900359	increases phosphorylation	1
triphenyl phosphate	affects expression	1
sodium arsenite	decreases expression	1
cobaltous chloride	increases expression	1
CGP 52608	affects binding, increases reaction	1
2-palmitoylglycerol	increases expression	1
abrine	decreases expression	1
Grape Seed Proanthocyanidins	affects cotreatment, increases expression	1
jinfukang	increases expression	1
Leflunomide	decreases expression	1
Benzo(a)pyrene	affects methylation	1
Catechin	affects cotreatment, increases expression	1
Doxorubicin	decreases expression	1
Gallic Acid	decreases expression	1
Dronabinol	increases glycosylation	1
Tobacco Smoke Pollution	increases expression	1
Urethane	increases expression	1
Valproic Acid	increases methylation	1
Antirheumatic Agents	affects expression	1
Cadmium Chloride	decreases expression	1

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_XW79	HEK293 eGFP-ZNF71	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.