ZNF732

Summary

ZNF732 (zinc finger protein 732, HGNC:37138) is a protein-coding gene on chromosome 4p16.3, encoding Zinc finger protein 732 (B4DXR9). May be involved in transcriptional regulation.

This gene encodes a kruppel-associated box-containing zinc finger protein (KRAB-ZFP). The encoded protein contains an N-terminal kruppel-associated box (KRAB) domain and sixteen C-terminal C2H2-type zinc finger domains. The KRAB-ZFPs represent the largest family of mammalian transcriptional repressors, which function through the recruitment of the nuclear co-factor KRAB-Associated Protein 1 (KAP1), to engage histone modifiers and induce heterochromatin formation.

Source: NCBI Gene 654254 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 109 total
• MANE Select transcript: NM_001137608

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:37138
Approved symbol	ZNF732
Name	zinc finger protein 732
Location	4p16.3
Locus type	gene with protein product
Status	Approved
Aliases	FLJ59067
Ensembl gene	ENSG00000186777
Ensembl biotype	protein_coding
Entrez	654254

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000419098

RefSeq mRNA: 1 — MANE Select: NM_001137608 NM_001137608

CCDS: CCDS46990

Canonical transcript exons

ENST00000419098 — 4 exons

Exon	Start	End
ENSE00001608370	305308	305474
ENSE00001643036	270675	272630
ENSE00001664041	295438	295533
ENSE00001674937	296029	296155

Expression profiles

Bgee: expression breadth ubiquitous, 120 present calls, max score 80.99.

FANTOM5 (CAGE): breadth broad, TPM avg 1.5965 / max 62.0633, expressed in 461 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
50983	1.5965	461

Top tissues by expression

130 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	80.99	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	77.76	gold quality
ventricular zone	UBERON:0003053	69.04	gold quality
tonsil	UBERON:0002372	61.47	gold quality
ganglionic eminence	UBERON:0004023	61.13	gold quality
cerebellar cortex	UBERON:0002129	60.66	gold quality
cerebellar hemisphere	UBERON:0002245	60.62	gold quality
cerebellum	UBERON:0002037	60.48	gold quality
endometrium	UBERON:0001295	59.71	gold quality
right hemisphere of cerebellum	UBERON:0014890	59.69	gold quality
lower esophagus mucosa	UBERON:0035834	59.27	gold quality
primary visual cortex	UBERON:0002436	56.97	gold quality
colonic epithelium	UBERON:0000397	56.93	silver quality
calcaneal tendon	UBERON:0003701	56.51	gold quality
cortical plate	UBERON:0005343	55.87	gold quality
placenta	UBERON:0001987	55.85	gold quality
left ovary	UBERON:0002119	55.01	gold quality
ovary	UBERON:0000992	54.50	gold quality
body of uterus	UBERON:0009853	54.18	gold quality
lymph node	UBERON:0000029	53.90	gold quality
islet of Langerhans	UBERON:0000006	53.88	gold quality
superior frontal gyrus	UBERON:0002661	53.51	gold quality
nucleus accumbens	UBERON:0001882	53.46	gold quality
myometrium	UBERON:0001296	53.45	gold quality
urinary bladder	UBERON:0001255	53.32	gold quality
uterine cervix	UBERON:0000002	53.15	gold quality
adrenal tissue	UBERON:0018303	53.12	gold quality
pituitary gland	UBERON:0000007	52.78	gold quality
adenohypophysis	UBERON:0002196	52.27	gold quality
brain	UBERON:0000955	51.84	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.06

Regulation

Is transcription factor: yes

JASPAR motifs

Motif	Name	Family
MA2528.1	ZNF732	More than 3 adjacent zinc fingers

JASPAR matrix evidence (PMIDs): PMID:39605530

miRNA regulators (miRDB)

46 targeting ZNF732, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3064-3P	100.00	70.09	1254
HSA-MIR-188-3P	100.00	68.76	1240
HSA-MIR-3925-3P	100.00	69.95	1237
HSA-MIR-4776-3P	100.00	68.73	1340
HSA-MIR-12121	99.99	66.64	255
HSA-MIR-4715-3P	99.98	66.03	670
HSA-MIR-23A-3P	99.95	74.24	3163
HSA-MIR-23B-3P	99.95	74.24	3163
HSA-MIR-23C	99.95	73.92	3192
HSA-MIR-4713-5P	99.78	67.80	1794
HSA-MIR-3156-3P	99.76	66.72	939
HSA-MIR-2116-3P	99.74	64.32	889
HSA-MIR-10393-5P	99.65	68.01	1368
HSA-MIR-1251-3P	99.64	67.21	1408
HSA-MIR-6126	99.62	68.09	996
HSA-MIR-1260A	99.61	66.67	1098
HSA-MIR-1260B	99.61	66.67	1098
HSA-MIR-136-5P	99.50	67.26	1153
HSA-MIR-6833-5P	99.50	68.93	1161
HSA-MIR-766-3P	99.47	65.24	1811
HSA-MIR-6853-3P	99.36	70.79	1558
HSA-MIR-296-3P	99.21	66.56	474
HSA-MIR-4464	98.95	67.73	820
HSA-MIR-4748	98.95	67.53	810
HSA-MIR-412-3P	98.86	66.89	712
HSA-MIR-6754-3P	98.84	66.60	889
HSA-MIR-4751	98.80	64.95	525
HSA-MIR-11399	98.71	65.69	869
HSA-MIR-4752	98.71	68.04	833
HSA-MIR-6837-3P	98.42	66.71	1149

Cross-species orthologs

0 orthologs

Paralogs (176): ZNF195 (ENSG00000005801), ZNF112 (ENSG00000062370), ZNF275 (ENSG00000063587), ZNF37A (ENSG00000075407), ZNF510 (ENSG00000081386), ZNF506 (ENSG00000081665), ZNF268 (ENSG00000090612), MZF1 (ENSG00000099326), ZNF629 (ENSG00000102870), ZNF175 (ENSG00000105497), ZNF85 (ENSG00000105750), ZFP30 (ENSG00000120784), ZNF45 (ENSG00000124459), ZNF391 (ENSG00000124613), ZNF436 (ENSG00000125945), ZNF484 (ENSG00000127081), ZNF835 (ENSG00000127903), ZNF780B (ENSG00000128000), ZSCAN10 (ENSG00000130182), ZNF317 (ENSG00000130803), ZNF331 (ENSG00000130844), ZNF227 (ENSG00000131115), ZNF141 (ENSG00000131127), ZNF132 (ENSG00000131849), ZNF189 (ENSG00000136870), ZIM3 (ENSG00000141946), ZFP14 (ENSG00000142065), ZNF514 (ENSG00000144026), ZNF300 (ENSG00000145908), RBAK (ENSG00000146587), ZNF157 (ENSG00000147117), ZNF182 (ENSG00000147118), ZNF41 (ENSG00000147124), ZNF7 (ENSG00000147789), ZNF117 (ENSG00000152926), ZNF221 (ENSG00000159905), ZNF235 (ENSG00000159917), ZNF714 (ENSG00000160352), ZNF577 (ENSG00000161551), ZNF12 (ENSG00000164631)

Protein

Protein identifiers

Zinc finger protein 732 — B4DXR9 (reviewed: B4DXR9)

All UniProt accessions (1): B4DXR9

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (1): NP_001131080* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily

Pfam: PF00096, PF01352, PF13912

UniProt features (18 total): zinc finger region 16, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-B4DXR9-F1	69.25	0.13

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 37 (showing top): chr4p16, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, GOBP_NEGATIVE_REGULATION_OF_TRANSCRIPTION_BY_RNA_POLYMERASE_II, GOBP_NEGATIVE_REGULATION_OF_NUCLEOBASE_CONTAINING_COMPOUND_METABOLIC_PROCESS, ZWANG_DOWN_BY_2ND_EGF_PULSE, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, HMGA1_TARGET_GENES, HSD17B8_TARGET_GENES, PRKDC_TARGET_GENES, MIR3925_3P, MIR3064_3P, MIR188_3P, MIR4776_3P, MIR766_3P, MIR1251_3P

GO Biological Process (2): negative regulation of transcription by RNA polymerase II (GO:0000122), regulation of DNA-templated transcription (GO:0006355)

GO Molecular Function (6): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), double-stranded DNA binding (GO:0003690), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of transcription by RNA polymerase II	2
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
transcription by RNA polymerase II	1
negative regulation of DNA-templated transcription	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
transition metal ion binding	1
nucleic acid binding	1
DNA binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

184 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF732	ANKS4B	Q8N8V4	505
ZNF732	TRAPPC12	Q8WVT3	447
ZNF732	SNTG2	Q9NY99	407
ZNF732	MAGEB2	O15479	402
ZNF732	POLN	Q7Z5Q5	400
ZNF732	SUSD1	Q6UWL2	393
ZNF732	HDHD2	Q9H0R4	371
ZNF732	CCDC7	Q96M83	371
ZNF732	TMEM35B	Q8NCS4	348
ZNF732	PHYHD1	Q5SRE7	334
ZNF732	FBXL13	Q8NEE6	320
ZNF732	MRTFB	Q9ULH7	318
ZNF732	CCDC8	Q9H0W5	273
ZNF732	AKNA	Q7Z591	267
ZNF732	ZCCHC8	Q6NZY4	264

IntAct

8 interactions, top by confidence:

A	B	Type	Score
TRIM28	ZNF320	psi-mi:“MI:0914”(association)	0.530
ECE1	ZNF732	psi-mi:“MI:0915”(physical association)	0.370
TRIM28	ZNF320	psi-mi:“MI:0914”(association)	0.350
PURG	ZNF320	psi-mi:“MI:0914”(association)	0.350
MCEE	ZNF732	psi-mi:“MI:0914”(association)	0.350

BioGRID (4): ZNF732 (Affinity Capture-MS), ZNF732 (Affinity Capture-MS), ZNF732 (Affinity Capture-MS), ZNF732 (Affinity Capture-MS)

ESM2 similar proteins: A6NDX5, A6NK75, A6NNF4, A6NP11, B4DXR9, B7Z6K7, C9JN71, O43361, O75290, O75373, P0CJ79, P0DKX0, P17019, P17021, P17035, P17039, P35789, Q02386, Q03938, Q08AN1, Q13106, Q15928, Q3ZCX4, Q5R9F0, Q5SXM1, Q68EA5, Q6P9A1, Q6PDB4, Q6ZN06, Q6ZN08, Q6ZNG1, Q76KX8, Q86V71, Q86XN6, Q8IYB9, Q8N4W9, Q8NEP9, Q8TBZ8, Q8TC21, Q8TF32

Diamond homologs: A0A1W2PQL4, A6NK75, A6NN14, A6NNF4, A6NP11, A8MQ14, A8MTY0, A8MUV8, A8MXY4, B4DX44, B4DXR9, E9PYI1, O14628, O43345, O75290, O75346, O75373, O75437, O95780, P0CB33, P0DKX0, P0DPD5, P16373, P17019, P17038, P21506, P35789, P52736, P52738, P52744, Q03923, Q03924, Q03936, Q03938, Q05481, Q14586, Q14593, Q14929, Q15928, Q3KNS6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

109 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	96
Likely benign	11
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

687 predictions. Top by Δscore:

Variant	Effect	Δscore
4:296012:A:AC	donor_gain	1.0000
4:296013:C:CC	donor_gain	1.0000
4:272626:CACAG:C	acceptor_gain	0.9900
4:272628:CAG:C	acceptor_gain	0.9900
4:295432:ACCT:A	donor_loss	0.9900
4:295433:CCTA:C	donor_loss	0.9900
4:295434:CT:C	donor_loss	0.9900
4:295435:TACCT:T	donor_loss	0.9900
4:295436:ACCTG:A	donor_loss	0.9900
4:295437:CC:C	donor_loss	0.9900
4:295532:ACCTG:A	acceptor_loss	0.9900
4:295533:CCTG:C	acceptor_loss	0.9900
4:296023:CCTCA:C	donor_loss	0.9900
4:296024:CTCAC:C	donor_loss	0.9900
4:296027:A:AG	donor_loss	0.9900
4:296028:CCCAG:C	donor_loss	0.9900
4:272631:C:CC	acceptor_gain	0.9800
4:295430:TCAC:T	donor_loss	0.9800
4:295431:CACC:C	donor_loss	0.9800
4:295536:G:C	acceptor_loss	0.9800
4:295986:C:CA	donor_gain	0.9800
4:296021:AT:A	donor_gain	0.9800
4:296021:ATCCT:A	donor_loss	0.9800
4:295438:C:A	donor_loss	0.9700
4:295531:CAC:C	acceptor_gain	0.9700
4:296027:AC:A	donor_gain	0.9700
4:296028:CC:C	donor_gain	0.9700
4:295429:GTCAC:G	donor_loss	0.9600
4:296044:T:TA	donor_gain	0.9600
4:296154:TCC:T	acceptor_loss	0.9600

AlphaMissense

3893 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
4:271828:A:C	F343L	0.996
4:271828:A:T	F343L	0.996
4:271830:A:G	F343L	0.996
4:271744:A:C	F371L	0.992
4:271744:A:T	F371L	0.992
4:271746:A:G	F371L	0.992
4:271660:A:C	F399L	0.988
4:271660:A:T	F399L	0.988
4:271662:A:G	F399L	0.988
4:271492:A:C	F455L	0.987
4:271492:A:T	F455L	0.987
4:271494:A:G	F455L	0.987
4:271324:A:C	F511L	0.979
4:271324:A:T	F511L	0.979
4:271326:A:G	F511L	0.979
4:271727:A:G	L377P	0.978
4:271829:A:G	F343S	0.974
4:271576:A:C	F427L	0.973
4:271576:A:T	F427L	0.973
4:271578:A:G	F427L	0.973
4:296120:G:C	F13L	0.973
4:296120:G:T	F13L	0.973
4:296122:A:G	F13L	0.973
4:271408:A:C	F483L	0.972
4:271408:A:T	F483L	0.972
4:271410:A:G	F483L	0.972
4:271705:A:C	H384Q	0.965
4:271705:A:T	H384Q	0.965
4:271739:T:G	Q373P	0.964
4:271912:A:C	F315L	0.964

dbSNP variants (sampled 300 via entrez): RS1000035875 (4:287108 G>A,T), RS1000091614 (4:295889 C>T), RS1000189243 (4:289891 T>G), RS1000237308 (4:283861 A>G), RS10002733 (4:285607 A>C), RS10003142 (4:297970 C>A,G,T), RS10005733 (4:298212 C>A,G), RS1000613684 (4:277983 A>C), RS1000708285 (4:289601 G>A,T), RS1000720571 (4:271764 C>T), RS1000876669 (4:300961 A>T), RS1000985570 (4:294970 G>A,C), RS1001101484 (4:294605 G>C), RS1001270569 (4:302928 C>T), RS1001357061 (4:290243 C>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

6 total (human), top 6 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases methylation	1
sodium arsenite	decreases expression	1
potassium chromate(VI)	increases expression	1
Benzo(a)pyrene	affects methylation	1
Cadmium Chloride	decreases expression	1
p-Chloromercuribenzoic Acid	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.