ZNF735

Summary

ZNF735 (zinc finger protein 735, HGNC:32466) is a protein-coding gene on chromosome 7q11.21, encoding Zinc finger protein 735 (P0CB33). May be involved in transcriptional regulation.

This gene encodes a kruppel-associated box-containing zinc finger protein (KRAB-ZFP). The encoded protein contains an N-terminal kruppel-associated box (KRAB) domain and nine C-terminal C2H2-type zinc finger domains. The KRAB-ZFPs represent the largest family of mammalian transcriptional repressors, which function through the recruitment of the nuclear co-factor KRAB-Associated Protein 1 (KAP1), to engage histone modifiers and induce heterochromatin formation.

Source: NCBI Gene 730291 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 70 total
• MANE Select transcript: NM_001159524

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:32466
Approved symbol	ZNF735
Name	zinc finger protein 735
Location	7q11.21
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000223614
Ensembl biotype	protein_coding
Entrez	730291

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000429565

RefSeq mRNA: 1 — MANE Select: NM_001159524 NM_001159524

CCDS: CCDS78236

Canonical transcript exons

ENST00000429565 — 4 exons

Exon	Start	End
ENSE00001714193	64219314	64220290
ENSE00002467305	64214013	64214108
ENSE00002519157	64213092	64213218
ENSE00003758512	64207203	64207241

Expression profiles

Bgee: expression breadth not_expressed, 0 present calls, max score 37.20.

Top tissues by expression

127 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
colonic epithelium	UBERON:0000397	37.20	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
bone marrow cell	CL:0002092	36.16	gold quality
ganglionic eminence	UBERON:0004023	35.49	gold quality
skeletal muscle tissue	UBERON:0001134	33.38	gold quality
muscle tissue	UBERON:0002385	32.15	gold quality
hindlimb stylopod muscle	UBERON:0004252	32.15	gold quality
bone marrow	UBERON:0002371	31.74	gold quality
sural nerve	UBERON:0015488	30.93	gold quality
stromal cell of endometrium	CL:0002255	29.87	gold quality
prefrontal cortex	UBERON:0000451	29.04	gold quality
monocyte	CL:0000576	28.26	gold quality
leukocyte	CL:0000738	28.20	gold quality
duodenum	UBERON:0002114	28.14	gold quality
liver	UBERON:0002107	28.04	gold quality
lymph node	UBERON:0000029	27.57	gold quality
tonsil	UBERON:0002372	27.05	gold quality
superior frontal gyrus	UBERON:0002661	26.91	gold quality
urinary bladder	UBERON:0001255	26.74	gold quality
blood	UBERON:0000178	26.58	gold quality
islet of Langerhans	UBERON:0000006	26.55	gold quality
vermiform appendix	UBERON:0001154	26.42	gold quality
gall bladder	UBERON:0002110	25.98	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	25.89	gold quality
placenta	UBERON:0001987	25.81	gold quality
muscle of leg	UBERON:0001383	25.04	gold quality
primary visual cortex	UBERON:0002436	24.61	gold quality
frontal cortex	UBERON:0001870	24.57	gold quality
pancreas	UBERON:0001264	24.34	gold quality
endometrium	UBERON:0001295	23.91	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.12

Regulation

Is transcription factor: no

Cross-species orthologs

0 orthologs

Paralogs (176): ZNF195 (ENSG00000005801), ZNF112 (ENSG00000062370), ZNF275 (ENSG00000063587), ZNF37A (ENSG00000075407), ZNF510 (ENSG00000081386), ZNF506 (ENSG00000081665), ZNF268 (ENSG00000090612), MZF1 (ENSG00000099326), ZNF629 (ENSG00000102870), ZNF175 (ENSG00000105497), ZNF85 (ENSG00000105750), ZFP30 (ENSG00000120784), ZNF45 (ENSG00000124459), ZNF391 (ENSG00000124613), ZNF436 (ENSG00000125945), ZNF484 (ENSG00000127081), ZNF835 (ENSG00000127903), ZNF780B (ENSG00000128000), ZSCAN10 (ENSG00000130182), ZNF317 (ENSG00000130803), ZNF331 (ENSG00000130844), ZNF227 (ENSG00000131115), ZNF141 (ENSG00000131127), ZNF132 (ENSG00000131849), ZNF189 (ENSG00000136870), ZIM3 (ENSG00000141946), ZFP14 (ENSG00000142065), ZNF514 (ENSG00000144026), ZNF300 (ENSG00000145908), RBAK (ENSG00000146587), ZNF157 (ENSG00000147117), ZNF182 (ENSG00000147118), ZNF41 (ENSG00000147124), ZNF7 (ENSG00000147789), ZNF117 (ENSG00000152926), ZNF221 (ENSG00000159905), ZNF235 (ENSG00000159917), ZNF714 (ENSG00000160352), ZNF577 (ENSG00000161551), ZNF12 (ENSG00000164631)

Protein

Protein identifiers

Zinc finger protein 735 — P0CB33 (reviewed: P0CB33)

All UniProt accessions (1): P0CB33

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (1): NP_001152996* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050589	Ikaros_C2H2-ZF	Family

Pfam: PF00096, PF01352

UniProt features (11 total): zinc finger region 9, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P0CB33-F1	68.42	0.16

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 7 (showing top): chr7q11, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, GOMF_DNA_BINDING_TRANSCRIPTION_FACTOR_ACTIVITY, GOMF_CIS_REGULATORY_REGION_SEQUENCE_SPECIFIC_DNA_BINDING, GOMF_RNA_POLYMERASE_II_TRANSCRIPTION_REGULATORY_REGION_SEQUENCE_SPECIFIC_DNA_BINDING, REACTOME_RNA_POLYMERASE_II_TRANSCRIPTION

GO Biological Process (2): regulation of DNA-templated transcription (GO:0006355), regulation of transcription by RNA polymerase II (GO:0006357)

GO Molecular Function (5): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

102 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF735	SEC14L6	B5MCN3	480
ZNF735	OR52E4	Q8NGH9	469
ZNF735	BPY2	O14599	447
ZNF735	USP17L1	Q7RTZ2	393
ZNF735	TRIM60	Q495X7	375
ZNF735	SPIDR	Q14159	337
ZNF735	SEC14L4	Q9UDX3	310
ZNF735	HGSNAT	Q68CP4	305
ZNF735	RBMY1D	P0C7P1	305
ZNF735	FNTA	P49354	299
ZNF735	VCY	O14598	273
ZNF735	POMK	Q9H5K3	273
ZNF735	DAZ2	Q13117	273
ZNF735	HOOK3	Q86VS8	263
ZNF735	MAP1S	Q66K74	234

IntAct

2 interactions, top by confidence:

A	B	Type	Score
ZNF735	ECE1	psi-mi:“MI:0915”(physical association)	0.370

BioGRID (1): ZNF735 (Affinity Capture-MS)

ESM2 similar proteins: A0A1W2PQL4, A0JPL0, A2RRD8, A6NHJ4, A6NP11, B4DX44, O75346, O95780, P0CB33, P21506, P51508, P52738, Q0D2J5, Q0VGE8, Q12901, Q13360, Q14593, Q15973, Q2M218, Q2M3X9, Q2VY69, Q3SXZ3, Q5HY98, Q5RCJ2, Q5VIY5, Q6J6I6, Q7L2R6, Q86XU0, Q86Y25, Q8IW36, Q8IYN0, Q8IYX0, Q8N782, Q8N883, Q8N9Z0, Q8NDP4, Q95K49, Q969W8, Q96H40, Q96N58

Diamond homologs: A0A1W2PQL4, A6NK75, A6NN14, A6NNF4, A6NP11, A8MQ14, A8MTY0, A8MUV8, A8MXY4, B4DX44, B4DXR9, E9PYI1, O14628, O43345, O75290, O75346, O75373, O75437, O95780, P0CB33, P0DKX0, P0DPD5, P16373, P17019, P17038, P21506, P35789, P52736, P52738, P52744, Q03923, Q03924, Q03936, Q03938, Q05481, Q14586, Q14593, Q14929, Q15928, Q3KNS6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

70 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	60
Likely benign	7
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

495 predictions. Top by Δscore:

Variant	Effect	Δscore
7:64207239:ATG:A	donor_gain	1.0000
7:64207239:ATGGT:A	donor_loss	1.0000
7:64207240:TG:T	donor_gain	1.0000
7:64207240:TGGTG:T	donor_loss	1.0000
7:64207241:GG:G	donor_gain	1.0000
7:64207242:G:GG	donor_gain	1.0000
7:64207242:GTG:G	donor_loss	1.0000
7:64219312:A:AG	acceptor_gain	1.0000
7:64219313:G:GA	acceptor_gain	1.0000
7:64219313:GTTAC:G	acceptor_gain	1.0000
7:64207238:AATG:A	donor_gain	0.9900
7:64213086:TTTCA:T	acceptor_loss	0.9900
7:64213087:TTCA:T	acceptor_loss	0.9900
7:64213088:TCA:T	acceptor_loss	0.9900
7:64213090:A:AC	acceptor_loss	0.9900
7:64213090:A:AG	acceptor_gain	0.9900
7:64213090:AG:A	acceptor_gain	0.9900
7:64213090:AGG:A	acceptor_gain	0.9900
7:64213091:G:GG	acceptor_gain	0.9900
7:64213091:GG:G	acceptor_gain	0.9900
7:64213091:GGG:G	acceptor_gain	0.9900
7:64213091:GGGA:G	acceptor_gain	0.9900
7:64213091:GGGAC:G	acceptor_gain	0.9900
7:64213217:GG:G	donor_gain	0.9900
7:64213218:GG:G	donor_gain	0.9900
7:64213218:GGTGA:G	donor_loss	0.9900
7:64213219:G:GG	donor_gain	0.9900
7:64213219:GTGA:G	donor_loss	0.9900
7:64213220:T:A	donor_loss	0.9900
7:64219308:TTTCA:T	acceptor_loss	0.9900

AlphaMissense

2739 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:64220219:T:C	F390L	0.991
7:64220221:T:A	F390L	0.991
7:64220221:T:G	F390L	0.991
7:64220051:T:C	F334L	0.989
7:64220053:T:A	F334L	0.989
7:64220053:T:G	F334L	0.989
7:64219883:T:C	F278L	0.988
7:64219885:T:A	F278L	0.988
7:64219885:T:G	F278L	0.988
7:64219967:T:C	F306L	0.988
7:64219969:T:A	F306L	0.988
7:64219969:T:G	F306L	0.988
7:64219799:T:C	F250L	0.986
7:64219801:T:A	F250L	0.986
7:64219801:T:G	F250L	0.986
7:64220135:T:C	F362L	0.970
7:64220137:T:A	F362L	0.970
7:64220137:T:G	F362L	0.970
7:64213125:T:C	F25L	0.966
7:64213127:C:A	F25L	0.966
7:64213127:C:G	F25L	0.966
7:64219890:G:C	R280P	0.965
7:64220176:T:A	H375Q	0.951
7:64220176:T:G	H375Q	0.951
7:64220220:T:C	F390S	0.950
7:64213173:T:G	Y41D	0.948
7:64220260:C:A	H403Q	0.945
7:64220260:C:G	H403Q	0.945
7:64220219:T:A	F390I	0.938
7:64219968:T:C	F306S	0.937

dbSNP variants (sampled 300 via entrez): RS1000205638 (7:64210446 G>A), RS1000306126 (7:64215421 T>C), RS1000306281 (7:64205211 C>A), RS1000707413 (7:64215139 GCAA>G), RS1000959565 (7:64220751 T>C), RS1002039598 (7:64210717 A>G), RS1002592562 (7:64211930 T>C), RS1002887866 (7:64216774 T>G), RS1003042823 (7:64211698 T>C), RS1003578659 (7:64207932 A>G), RS1003927065 (7:64207484 T>G), RS1004398808 (7:64212206 A>C,G), RS1004736973 (7:64217346 G>C), RS1005327287 (7:64213635 C>G), RS1005329419 (7:64218614 A>G)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

2 total (human), top 2 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression	1
Benzo(a)pyrene	decreases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.