ZNF77

Summary

ZNF77 (zinc finger protein 77, HGNC:13150) is a protein-coding gene on chromosome 19p13.3, encoding Zinc finger protein 77 (Q15935). May be involved in transcriptional regulation.

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.

Source: NCBI Gene 58492 — RefSeq curated summary.

At a glance

• GWAS associations: 5
• Clinical variants (ClinVar): 122 total
• MANE Select transcript: NM_021217

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:13150
Approved symbol	ZNF77
Name	zinc finger protein 77
Location	19p13.3
Locus type	gene with protein product
Status	Approved
Aliases	pT1
Ensembl gene	ENSG00000175691
Ensembl biotype	protein_coding
OMIM	194551
Entrez	58492

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 3 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000314531, ENST00000588050, ENST00000863233, ENST00000915162

RefSeq mRNA: 3 — MANE Select: NM_021217 NM_001426550, NM_001426551, NM_021217

CCDS: CCDS12099

Canonical transcript exons

ENST00000314531 — 4 exons

Exon	Start	End
ENSE00001233594	2933218	2934815
ENSE00001331858	2936524	2936704
ENSE00001777165	2939281	2939407
ENSE00002818099	2944838	2944971

Expression profiles

Bgee: expression breadth ubiquitous, 184 present calls, max score 91.77.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 3.8812 / max 104.1237, expressed in 1499 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
178217	3.8812	1499

Top tissues by expression

249 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
oocyte	CL:0000023	91.77	gold quality
secondary oocyte	CL:0000655	88.65	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	86.78	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	83.16	gold quality
apex of heart	UBERON:0002098	75.46	gold quality
amniotic fluid	UBERON:0000173	75.27	silver quality
granulocyte	CL:0000094	74.96	gold quality
upper arm skin	UBERON:0004263	74.92	gold quality
ganglionic eminence	UBERON:0004023	74.80	gold quality
cortical plate	UBERON:0005343	74.46	gold quality
ventricular zone	UBERON:0003053	74.39	gold quality
islet of Langerhans	UBERON:0000006	73.61	gold quality
cerebellar hemisphere	UBERON:0002245	73.55	gold quality
cerebellar cortex	UBERON:0002129	73.51	gold quality
stromal cell of endometrium	CL:0002255	72.83	gold quality
cerebellum	UBERON:0002037	72.59	gold quality
right hemisphere of cerebellum	UBERON:0014890	72.40	gold quality
smooth muscle tissue	UBERON:0001135	72.17	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	71.94	gold quality
gingival epithelium	UBERON:0001949	71.50	silver quality
leukocyte	CL:0000738	71.42	gold quality
monocyte	CL:0000576	71.40	gold quality
epithelial cell of pancreas	CL:0000083	71.33	gold quality
vastus lateralis	UBERON:0001379	71.13	gold quality
calcaneal tendon	UBERON:0003701	71.09	gold quality
body of stomach	UBERON:0001161	70.80	gold quality
quadriceps femoris	UBERON:0001377	70.80	gold quality
right adrenal gland	UBERON:0001233	70.64	gold quality
left ovary	UBERON:0002119	70.53	gold quality
ectocervix	UBERON:0012249	70.53	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.36

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

2 targets.

Target	Regulation
CDH1
CDH2

miRNA regulators (miRDB)

39 targeting ZNF77, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6833-3P	100.00	70.63	3197
HSA-MIR-4768-5P	100.00	69.49	2861
HSA-MIR-548AN	99.97	70.91	2817
HSA-MIR-302E	99.96	70.74	2669
HSA-MIR-6845-3P	99.94	66.88	1439
HSA-MIR-6508-5P	99.92	70.67	2465
HSA-MIR-5680	99.91	69.83	3421
HSA-MIR-302A-3P	99.89	71.23	1777
HSA-MIR-302B-3P	99.89	71.23	1777
HSA-MIR-302C-3P	99.89	71.20	1778
HSA-MIR-302D-3P	99.89	71.25	1777
HSA-MIR-8067	99.86	69.59	2260
HSA-MIR-373-3P	99.84	70.68	1668
HSA-MIR-520E-3P	99.84	70.55	1698
HSA-MIR-372-3P	99.83	70.58	1691
HSA-MIR-520A-3P	99.83	70.59	1687
HSA-MIR-520B-3P	99.83	70.56	1699
HSA-MIR-520C-3P	99.83	70.56	1699
HSA-MIR-520D-3P	99.83	70.78	1676
HSA-MIR-374C-5P	99.80	72.06	2910
HSA-MIR-655-3P	99.80	72.19	2909
HSA-MIR-33A-3P	99.70	70.27	3362
HSA-MIR-3685	99.62	68.83	1621
HSA-MIR-4649-3P	99.56	66.90	1783
HSA-MIR-6083	99.47	68.73	2393
HSA-MIR-329-5P	99.27	68.11	1597
HSA-MIR-146A-3P	99.13	68.99	1881
HSA-MIR-3145-3P	98.85	69.07	2031
HSA-MIR-519A-2-5P	98.78	71.74	1401
HSA-MIR-520B-5P	98.78	71.74	1401

Literature-anchored findings (GeneRIF, showing 1)

• ZNF77 as a key controller of Aspergillus colonization and suggest its utility as a risk-marker for patient stratification. (PMID:30237437)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
mus_musculus	BC025920	ENSMUSG00000074862
drosophila_melanogaster	CG2712	FBGN0024975
drosophila_melanogaster	Phs	FBGN0036522
drosophila_melanogaster	CG3281	FBGN0260741

Paralogs (62): ZNF582 (ENSG00000018869), ZNF264 (ENSG00000083844), ZNF343 (ENSG00000088876), ZNF684 (ENSG00000117010), ZNF133 (ENSG00000125846), ZNF557 (ENSG00000130544), ZNF337 (ENSG00000130684), ZNF20 (ENSG00000132010), ZFP37 (ENSG00000136866), ZNF614 (ENSG00000142556), KRBOX4 (ENSG00000147121), ZNF599 (ENSG00000153896), ZNF19 (ENSG00000157429), ZNF589 (ENSG00000164048), PRDM9 (ENSG00000164256), ZNF180 (ENSG00000167384), ZNF558 (ENSG00000167785), ZNF35 (ENSG00000169981), ZNF778 (ENSG00000170100), ZNF439 (ENSG00000171291), ZNF440 (ENSG00000171295), ZNF556 (ENSG00000172000), ZNF554 (ENSG00000172006), ZNF596 (ENSG00000172748), ZNF80 (ENSG00000174255), ZNF266 (ENSG00000174652), ZNF25 (ENSG00000175395), ZNF169 (ENSG00000175787), ZNF404 (ENSG00000176222), ZNF491 (ENSG00000177599), ZNF620 (ENSG00000177842), ZNF619 (ENSG00000177873), ZNF875 (ENSG00000181666), ZNF329 (ENSG00000181894), ZFP90 (ENSG00000184939), ZNF566 (ENSG00000186017), ZNF529 (ENSG00000186020), ZNF749 (ENSG00000186230), ZNF555 (ENSG00000186300), ZNF70 (ENSG00000187792)

Protein

Protein identifiers

Zinc finger protein 77 — Q15935 (reviewed: Q15935)

Alternative names: ZNFpT1

All UniProt accessions (1): Q15935

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (3): NP_001413479, NP_001413480, NP_067040* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050752	C2H2-ZF_domain	Family

Pfam: PF00096, PF01352, PF13465, PF13894

UniProt features (20 total): zinc finger region 12, sequence variant 5, chain 1, domain 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q15935-F1	68.67	0.42

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 52 (showing top): GRYDER_PAX3FOXO1_ENHANCERS_IN_TADS, chr19p13, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, GRYDER_PAX3FOXO1_ENHANCERS_KO_DOWN, PURBEY_TARGETS_OF_CTBP1_NOT_SATB1_UP, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY, ARNT2_TARGET_GENES, ASH1L_TARGET_GENES, CEBPZ_TARGET_GENES, CHAF1B_TARGET_GENES, CREB3L4_TARGET_GENES, GREB1_TARGET_GENES, HES4_TARGET_GENES, NKX2_2_TARGET_GENES, PRKDC_TARGET_GENES

GO Biological Process (2): regulation of transcription by RNA polymerase II (GO:0006357), regulation of DNA-templated transcription (GO:0006355)

GO Molecular Function (6): RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), zinc ion binding (GO:0008270), DNA binding (GO:0003677), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
DNA-templated transcription	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
transcription cis-regulatory region binding	1
chromatin	1
RNA polymerase II transcription regulatory region sequence-specific DNA binding	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transition metal ion binding	1
nucleic acid binding	1
binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

720 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF77	P2RX3	P56373	861
ZNF77	P2RX2	Q9UBL9	712
ZNF77	P2RX1	P51575	631
ZNF77	NISCH	Q9Y2I1	600
ZNF77	SLC38A3	Q99624	546
ZNF77	P2RX4	Q99571	493
ZNF77	SERF1A	O75920	479
ZNF77	SFMBT1	Q9UHJ3	449
ZNF77	GPKOW	Q92917	417
ZNF77	CXCL11	O14625	400
ZNF77	PAF1	Q8N7H5	397
ZNF77	ZNF79	Q15937	391
ZNF77	PTS	Q03393	381
ZNF77	PTN	P21246	370
ZNF77	CARD16	Q5EG05	369

IntAct

34 interactions, top by confidence:

A	B	Type	Score
ZNF77	MBD3L1	psi-mi:“MI:0915”(physical association)	0.560
ZNF77	FTO	psi-mi:“MI:0915”(physical association)	0.560
ZNF77	NICN1	psi-mi:“MI:0915”(physical association)	0.560
ZNF77	RUSC1	psi-mi:“MI:0915”(physical association)	0.560
ZNF77	METTL27	psi-mi:“MI:0915”(physical association)	0.560
PRDX3	ZNF77	psi-mi:“MI:0915”(physical association)	0.560
ZNF77	RSPO4	psi-mi:“MI:0915”(physical association)	0.560
VWCE	ZNF316	psi-mi:“MI:0914”(association)	0.530
TRIM28	ZNF320	psi-mi:“MI:0914”(association)	0.530
TRIM28	ZNF320	psi-mi:“MI:0914”(association)	0.350
FBLN5	ZNF320	psi-mi:“MI:0914”(association)	0.350
LTBP2	ZNF320	psi-mi:“MI:0914”(association)	0.350
FBLN2	ZNF316	psi-mi:“MI:0914”(association)	0.350
OC90	ZNF324	psi-mi:“MI:0914”(association)	0.350
LDLRAD1	ZNF316	psi-mi:“MI:0914”(association)	0.350
OIT3	WNT10B	psi-mi:“MI:0914”(association)	0.350
ZNF77	METTL27	psi-mi:“MI:0915”(physical association)	0.000
ZNF77	NICN1	psi-mi:“MI:0915”(physical association)	0.000
ZNF77	RUSC1	psi-mi:“MI:0915”(physical association)	0.000
ZNF77	PRDX3	psi-mi:“MI:0915”(physical association)	0.000
ZNF77	RSPO4	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (28): ZNF77 (Affinity Capture-MS), ZNF77 (Affinity Capture-MS), ZNF77 (Affinity Capture-RNA), ZNF77 (Two-hybrid), ZNF77 (Two-hybrid), ZNF77 (Two-hybrid), ZNF77 (Two-hybrid), ZNF77 (Two-hybrid), ZNF77 (Two-hybrid), ZNF77 (Two-hybrid), NICN1 (Two-hybrid), ZNF77 (Affinity Capture-RNA), ZNF77 (Affinity Capture-MS), ZNF77 (Affinity Capture-MS), ZNF77 (Affinity Capture-MS)

ESM2 similar proteins: A2VDP4, A6QLU5, O75123, O75820, O94892, P10076, P10077, P16415, P17014, P17021, P17027, P18714, Q08AN1, Q0VGE8, Q14584, Q14587, Q14588, Q147U1, Q15935, Q32M78, Q3MIS6, Q3ZCX4, Q52M93, Q5MCW4, Q5R4K8, Q5RB30, Q5RDX1, Q5REK1, Q62513, Q62981, Q68EA5, Q6GQR8, Q6P3V2, Q6P560, Q6ZN19, Q6ZNA1, Q76KX8, Q7TSI0, Q7Z3V5, Q80W31

Diamond homologs: A2VDP4, B7Z6K7, D3ZVT0, E7ETH6, E9Q8G5, O43296, O43361, O75290, O75467, O75820, P0CH99, P0CI00, P15621, P17021, P17023, P17024, P17030, P17032, P52740, P52741, Q02525, Q06732, Q08DG8, Q08ER8, Q0VCB0, Q13106, Q13398, Q14587, Q147U1, Q15935, Q2M218, Q2M3X9, Q32M78, Q3KQV3, Q3SY52, Q3V080, Q3ZCX4, Q4R882, Q4V8A8, Q5CZA5

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

122 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	113
Likely benign	4
Benign	3

Top pathogenic / likely-pathogenic (0)

SpliceAI

679 predictions. Top by Δscore:

Variant	Effect	Δscore
19:2936701:CAAT:C	acceptor_gain	1.0000
19:2939275:CCTTA:C	donor_loss	1.0000
19:2939276:CTTA:C	donor_loss	1.0000
19:2939277:TTACC:T	donor_loss	1.0000
19:2939278:TACCC:T	donor_loss	1.0000
19:2939279:A:AC	donor_gain	1.0000
19:2939279:AC:A	donor_gain	1.0000
19:2939280:C:A	donor_loss	1.0000
19:2939280:C:CC	donor_gain	1.0000
19:2939280:CC:C	donor_gain	1.0000
19:2944833:CTCA:C	donor_loss	1.0000
19:2944835:CA:C	donor_loss	1.0000
19:2944836:ACC:A	donor_loss	1.0000
19:2944837:C:A	donor_loss	1.0000
19:2936516:CCACT:C	donor_loss	0.9900
19:2936517:CACTC:C	donor_loss	0.9900
19:2936518:ACTCA:A	donor_loss	0.9900
19:2936519:CTCA:C	donor_loss	0.9900
19:2936520:TCA:T	donor_loss	0.9900
19:2936521:C:CG	donor_loss	0.9900
19:2936705:C:CC	acceptor_gain	0.9900
19:2939296:T:TA	donor_gain	0.9900
19:2944830:GCACT:G	donor_loss	0.9900
19:2944831:CACT:C	donor_loss	0.9900
19:2944832:ACTC:A	donor_loss	0.9900
19:2944836:A:AC	donor_gain	0.9900
19:2944837:C:CC	donor_gain	0.9900
19:2934813:CTT:C	acceptor_gain	0.9800
19:2936523:CCT:C	donor_gain	0.9800
19:2936700:ACAAT:A	acceptor_gain	0.9800

AlphaMissense

3615 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:2933708:G:C	F473L	0.996
19:2933708:G:T	F473L	0.996
19:2933710:A:G	F473L	0.996
19:2933792:G:C	F445L	0.996
19:2933792:G:T	F445L	0.996
19:2933794:A:G	F445L	0.996
19:2934128:G:C	F333L	0.996
19:2934128:G:T	F333L	0.996
19:2934130:A:G	F333L	0.996
19:2934296:G:C	F277L	0.996
19:2934296:G:T	F277L	0.996
19:2934298:A:G	F277L	0.996
19:2933960:G:C	F389L	0.993
19:2933960:G:T	F389L	0.993
19:2933962:A:G	F389L	0.993
19:2934044:G:C	F361L	0.993
19:2934044:G:T	F361L	0.993
19:2934046:A:G	F361L	0.993
19:2934212:G:C	F305L	0.993
19:2934212:G:T	F305L	0.993
19:2934214:A:G	F305L	0.993
19:2933540:G:C	F529L	0.992
19:2933540:G:T	F529L	0.992
19:2933542:A:G	F529L	0.992
19:2933765:A:C	H454Q	0.992
19:2933765:A:T	H454Q	0.992
19:2933760:C:G	R456P	0.991
19:2933837:A:C	H430Q	0.991
19:2933837:A:T	H430Q	0.991
19:2933849:G:C	H426Q	0.990

dbSNP variants (sampled 300 via entrez): RS1000016839 (19:2945494 C>T), RS1000208748 (19:2935597 C>T), RS1000237649 (19:2945321 G>A,C), RS1000534960 (19:2944800 C>G,T), RS1000540973 (19:2950001 C>T), RS1000580363 (19:2935794 A>G), RS1000674209 (19:2932769 G>A), RS1000687866 (19:2939918 T>A), RS1000720075 (19:2939744 C>A,T), RS1000847594 (19:2950416 C>A,T), RS1000851441 (19:2937762 T>C), RS1000962104 (19:2937670 G>A,C), RS1001018251 (19:2946712 C>T), RS1001205064 (19:2942389 T>C), RS1001355977 (19:2938511 G>A,C,T)

Disease associations

OMIM: gene MIM:194551 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

5 associations (top):

Study	Trait	p-value
GCST003518_101	Daytime sleep phenotypes	8.000000e-06
GCST009879_7	Coronary artery disease	3.000000e-28
GCST010476_7	Myocardial infarction	1.000000e-24
GCST010478_10	Chronic kidney disease	3.000000e-07
GCST010836_8	Ischemic stroke	1.000000e-08

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0007828	daytime rest measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

29 total (human), top 29 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	decreases expression, increases abundance, increases expression	3
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
Tobacco Smoke Pollution	increases expression	2
Valproic Acid	decreases expression, increases expression	2
Cadmium Chloride	increases expression	2
TAK-243	increases sumoylation	1
bisphenol A	increases methylation	1
trichostatin A	decreases expression	1
methylparaben	increases expression	1
beryllium sulfate	increases expression	1
potassium chromate(VI)	affects cotreatment, increases expression	1
epigallocatechin gallate	affects cotreatment, increases expression	1
15-acetyldeoxynivalenol	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
ICG 001	increases expression	1
abrine	increases expression	1
2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazophenyl)amide	affects cotreatment, decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
Sunitinib	increases expression	1
Arsenic	decreases expression, increases abundance, increases expression	1
Copper	affects binding, decreases expression	1
Disulfiram	affects binding, decreases expression	1
Doxorubicin	decreases expression	1
Formaldehyde	increases expression	1
Plant Oils	increases expression	1
Tetrachlorodibenzodioxin	affects cotreatment, decreases expression	1
Tretinoin	increases expression	1
Cyclosporine	increases expression	1
Acrylamide	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.