ZNF777

Summary

ZNF777 (zinc finger protein 777, HGNC:22213) is a protein-coding gene on chromosome 7q36.1, encoding Zinc finger protein 777 (Q9ULD5). May be involved in transcriptional repression.

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of DNA-templated transcription; negative regulation of cell population proliferation; and protein heterooligomerization. Located in nucleus.

Source: NCBI Gene 27153 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 119 total
• MANE Select transcript: NM_015694

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:22213
Approved symbol	ZNF777
Name	zinc finger protein 777
Location	7q36.1
Locus type	gene with protein product
Status	Approved
Aliases	KIAA1285
Ensembl gene	ENSG00000196453
Ensembl biotype	protein_coding
OMIM	619298
Entrez	27153

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 5 protein_coding

ENST00000247930, ENST00000867956, ENST00000867957, ENST00000936128, ENST00000948305

RefSeq mRNA: 1 — MANE Select: NM_015694 NM_015694

CCDS: CCDS43675

Canonical transcript exons

ENST00000247930 — 6 exons

Exon	Start	End
ENSE00000872315	149454111	149454237
ENSE00000872316	149450999	149451112
ENSE00000872317	149436575	149436826
ENSE00001233792	149431363	149432932
ENSE00001388230	149455177	149456037
ENSE00001947109	149460815	149461062

Expression profiles

Bgee: expression breadth ubiquitous, 219 present calls, max score 86.10.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.1944 / max 11.8253, expressed in 60 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
86732	0.1050	22
86731	0.0894	25

Top tissues by expression

242 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
endothelial cell	CL:0000115	86.10	gold quality
gastrocnemius	UBERON:0001388	83.36	gold quality
hindlimb stylopod muscle	UBERON:0004252	82.94	gold quality
muscle of leg	UBERON:0001383	82.45	gold quality
ventricular zone	UBERON:0003053	81.91	gold quality
cortical plate	UBERON:0005343	81.56	gold quality
embryo	UBERON:0000922	80.68	gold quality
ganglionic eminence	UBERON:0004023	80.68	gold quality
granulocyte	CL:0000094	80.29	gold quality
popliteal artery	UBERON:0002250	80.20	gold quality
tibial artery	UBERON:0007610	80.18	gold quality
prefrontal cortex	UBERON:0000451	79.84	gold quality
secondary oocyte	CL:0000655	79.66	gold quality
skin of leg	UBERON:0001511	79.62	gold quality
stromal cell of endometrium	CL:0002255	79.59	gold quality
skin of abdomen	UBERON:0001416	79.19	gold quality
aorta	UBERON:0000947	79.09	gold quality
body of uterus	UBERON:0009853	79.08	gold quality
lower esophagus muscularis layer	UBERON:0035833	79.05	gold quality
right frontal lobe	UBERON:0002810	79.04	gold quality
lower esophagus	UBERON:0013473	78.99	gold quality
apex of heart	UBERON:0002098	78.78	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	78.73	gold quality
right ovary	UBERON:0002118	78.72	gold quality
muscle layer of sigmoid colon	UBERON:0035805	78.70	gold quality
left ovary	UBERON:0002119	78.43	gold quality
anterior cingulate cortex	UBERON:0009835	78.25	gold quality
zone of skin	UBERON:0000014	78.16	gold quality
descending thoracic aorta	UBERON:0002345	77.99	gold quality
left uterine tube	UBERON:0001303	77.83	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.93

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): STAT1

miRNA regulators (miRDB)

24 targeting ZNF777, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-126-5P	100.00	72.71	3180
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-23B-5P	99.98	66.07	587
HSA-MIR-23A-5P	99.94	65.39	468
HSA-MIR-651-3P	99.94	73.48	5177
HSA-MIR-6780A-5P	99.88	66.69	2776
HSA-MIR-548AG	99.77	69.25	1492
HSA-MIR-548M	99.70	68.87	1749
HSA-MIR-548AI	99.69	69.24	1494
HSA-MIR-548BA	99.69	69.14	1514
HSA-MIR-570-5P	99.69	69.24	1494
HSA-MIR-29B-2-5P	99.67	68.98	1726
HSA-MIR-140-3P	99.04	67.69	1324
HSA-MIR-6830-5P	99.01	68.73	1884
HSA-MIR-5701	98.97	69.54	1502
HSA-MIR-2355-5P	98.83	65.51	1589
HSA-MIR-561-5P	98.25	68.13	1365
HSA-MIR-509-3P	98.12	67.25	612
HSA-MIR-6793-3P	97.66	65.78	1084
HSA-MIR-10400-5P	96.91	66.00	56
HSA-MIR-4467	96.51	64.44	69
HSA-MIR-5684	93.17	64.85	454
HSA-MIR-4743-5P	88.08	64.31	91

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Zfp777	ENSMUSG00000071477
rattus_norvegicus	Zfp777	ENSRNOG00000033639

Paralogs (51): WIZ (ENSG00000011451), ZNF416 (ENSG00000083817), MYNN (ENSG00000085274), PRDM4 (ENSG00000110851), PRDM2 (ENSG00000116731), ZBTB17 (ENSG00000116809), ZNF644 (ENSG00000122482), GZF1 (ENSG00000125812), ZNF426 (ENSG00000130818), ZNF287 (ENSG00000141040), ZNF697 (ENSG00000143067), ZNF687 (ENSG00000143373), ZNF214 (ENSG00000149050), ZNF547 (ENSG00000152433), ZNF776 (ENSG00000152443), ZNF230 (ENSG00000159882), ZNF222 (ENSG00000159885), ZNF233 (ENSG00000159915), ZNF333 (ENSG00000160961), ZNF319 (ENSG00000166188), ZNF592 (ENSG00000166716), ZNF646 (ENSG00000167395), ZNF507 (ENSG00000168813), ZNF768 (ENSG00000169957), ZNF417 (ENSG00000173480), ZNF408 (ENSG00000175213), ZBTB41 (ENSG00000177888), ZNF223 (ENSG00000178386), ZNF852 (ENSG00000178917), ZNF784 (ENSG00000179922), ZNF572 (ENSG00000180938), ZNF707 (ENSG00000181135), ZNF746 (ENSG00000181220), ZNF467 (ENSG00000181444), ZNF530 (ENSG00000183647), ZNF17 (ENSG00000186272), ZNF527 (ENSG00000189164), ZKSCAN7 (ENSG00000196345), ZNF34 (ENSG00000196378), ZNF774 (ENSG00000196391)

Protein

Protein identifiers

Zinc finger protein 777 — Q9ULD5 (reviewed: Q9ULD5)

All UniProt accessions (1): Q9ULD5

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional repression. Inhibits cell proliferation through CDKN1A/p21 induction by down-regulation of NIBAN1/FAM129A at low cell density.

Subunit / interactions. Heterooligomer with ZNF746.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (1): NP_056509* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001909	KRAB	Domain
IPR013087	Znf_C2H2_type	Domain
IPR036051	KRAB_dom_sf	Homologous_superfamily
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050331	Zinc_finger_PRDM4/PRDM1/PRDM14	Family

Pfam: PF00096, PF01352

UniProt features (33 total): zinc finger region 9, modified residue 6, region of interest 5, compositionally biased region 5, sequence variant 3, cross-link 2, chain 1, domain 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9ULD5-F1	58.74	0.23

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (8): 47, 157, 496, 502, 604, 623, 330, 452

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-212436	Generic Transcription Pathway

MSigDB gene sets: 82 (showing top): RNGTGGGC_UNKNOWN, TAATAAT_MIR126, PAX4_01, CAGCTG_AP4_Q5, MODULE_331, HNF4_01, LIAO_METASTASIS, DELASERNA_MYOD_TARGETS_DN, RGAGGAARY_PU1_Q6, MODULE_48, MODULE_95, GOBP_PROTEIN_HETEROOLIGOMERIZATION, HSF2_01, GOMF_SEQUENCE_SPECIFIC_DNA_BINDING, ATACTGT_MIR144

GO Biological Process (5): regulation of transcription by RNA polymerase II (GO:0006357), negative regulation of cell population proliferation (GO:0008285), negative regulation of DNA-templated transcription (GO:0045892), protein heterooligomerization (GO:0051291), regulation of DNA-templated transcription (GO:0006355)

GO Molecular Function (7): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), DNA-binding transcription factor activity (GO:0003700), zinc ion binding (GO:0008270), DNA binding (GO:0003677), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RNA Polymerase II Transcription	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of DNA-templated transcription	3
DNA-templated transcription	2
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
transcription by RNA polymerase II	1
cell population proliferation	1
regulation of cell population proliferation	1
negative regulation of cellular process	1
negative regulation of RNA biosynthetic process	1
protein complex oligomerization	1
regulation of gene expression	1
regulation of RNA biosynthetic process	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transcription cis-regulatory region binding	1
transcription regulator activity	1
transition metal ion binding	1
nucleic acid binding	1
binding	1
cation binding	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

938 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZNF777	C7orf33	Q8WU49	419
ZNF777	ZNF534	Q76KX8	416
ZNF777	DRC11L	A6NCM1	401
ZNF777	TTPAL	Q9BTX7	400
ZNF777	A0A087WTJ2	A0A087WTJ2	374
ZNF777	ZBED10P	Q96FA7	369
ZNF777	ZKSCAN2	Q63HK3	356
ZNF777	ZFHX2	Q9C0A1	351
ZNF777	LRRC61	Q9BV99	348
ZNF777	RNF24	Q9Y225	348
ZNF777	WDR86	Q86TI4	340
ZNF777	DNAJB14	Q8TBM8	332
ZNF777	FSIP2	Q5CZC0	311
ZNF777	GTF2IRD1	Q9UHL9	296
ZNF777	NIBAN1	Q9BZQ8	290

IntAct

97 interactions, top by confidence:

A	B	Type	Score
FAM9B	ZNF777	psi-mi:“MI:0915”(physical association)	0.670
KIAA0753	ZNF777	psi-mi:“MI:0915”(physical association)	0.560
ZNF777	CLK3	psi-mi:“MI:0915”(physical association)	0.560
	ZNF777	psi-mi:“MI:0915”(physical association)	0.560
KRTAP10-9	ZNF777	psi-mi:“MI:0915”(physical association)	0.560
CEP70	ZNF777	psi-mi:“MI:0915”(physical association)	0.560
TFIP11	ZNF777	psi-mi:“MI:0915”(physical association)	0.560
IL16	ZNF777	psi-mi:“MI:0915”(physical association)	0.560
FSD2	ZNF777	psi-mi:“MI:0915”(physical association)	0.560
ERP44	MEX3A	psi-mi:“MI:0914”(association)	0.530
RBM34	NVL	psi-mi:“MI:0914”(association)	0.530
ZNF398	LRP4	psi-mi:“MI:0914”(association)	0.530
ZBTB48	ZBTB24	psi-mi:“MI:0914”(association)	0.530
RPSA	DKC1	psi-mi:“MI:0914”(association)	0.530
RPS3	ZNF316	psi-mi:“MI:0914”(association)	0.530
MAGEB2	POLRMT	psi-mi:“MI:0914”(association)	0.530
FAM9B	GEMIN2	psi-mi:“MI:0914”(association)	0.530
VTN	HAT1	psi-mi:“MI:0914”(association)	0.530
ZC3HAV1	KHNYN	psi-mi:“MI:0914”(association)	0.530
ABT1	ZNF316	psi-mi:“MI:0914”(association)	0.530
ZC3HAV1	MPHOSPH10	psi-mi:“MI:0914”(association)	0.350
DGCR8	MPHOSPH10	psi-mi:“MI:0914”(association)	0.350
VTN	HAT1	psi-mi:“MI:0914”(association)	0.350
C6orf11	RRP8	psi-mi:“MI:0914”(association)	0.350
ZNF212	ZNF746	psi-mi:“MI:0914”(association)	0.350

BioGRID (115): ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-MS), ZNF777 (Affinity Capture-RNA), ZNF777 (Two-hybrid), ZNF777 (Two-hybrid)

ESM2 similar proteins: A0A1D5NS60, A0JN76, A1YFX5, A2T7G6, A6NJL1, D2HQI1, F1MJR8, O14901, P0CG00, P10754, P22227, P98182, Q0IJ29, Q1L8W0, Q3SWU4, Q5DW34, Q5EAC5, Q5EXX3, Q5RHB5, Q5SXI5, Q5T619, Q66H04, Q6NRM8, Q6NV66, Q6ZSB9, Q7M6U3, Q7TS63, Q7TSH3, Q7ZWZ4, Q801P1, Q86VK4, Q8BKX7, Q8BXX2, Q8NAM6, Q8NAP3, Q8NCP5, Q8R0A2, Q91VW9, Q96IT1, Q96N77

Diamond homologs: A0JPL0, A2VDP4, A3KN32, A6NFI3, A6NM28, A6QLU5, B2RXC5, B4DU55, O43296, O60765, O75290, O75467, P10072, P15622, P17014, P17023, P17030, P17031, P17032, P17098, P21506, P51508, P51523, P52736, P52738, Q02975, Q06730, Q06732, Q14590, Q14929, Q16587, Q29RZ4, Q2TL60, Q3KNS6, Q3KQV3, Q49AA0, Q4R8H9, Q4V8A8, Q4V8E9, Q5FWF6

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 89 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Peptide chain elongation	9	22.8×	9e-09
Viral mRNA Translation	9	22.8×	9e-09
PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA	9	22.6×	9e-09
Selenocysteine synthesis	9	21.6×	9e-09
Eukaryotic Translation Termination	9	21.6×	9e-09
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)	9	21.2×	9e-09
ZNF598 and the Ribosome-associated Quality Trigger (RQT) complex dissociate a ribosome stalled on a no-go mRNA	9	21.2×	9e-09
Formation of a pool of free 40S subunits	9	20.1×	1e-08

GO biological processes:

GO term	Partners	Fold	FDR
cytoplasmic translation	9	21.4×	2e-07
regulation of alternative mRNA splicing, via spliceosome	5	15.7×	3e-03
RNA processing	5	14.0×	4e-03
translation	9	11.9×	2e-05

Disease & clinical

Clinical variants and AI predictions

ClinVar

119 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	104
Likely benign	5
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

972 predictions. Top by Δscore:

Variant	Effect	Δscore
7:149432928:CCCCT:C	acceptor_gain	1.0000
7:149432929:CCCTC:C	acceptor_gain	1.0000
7:149432933:C:A	acceptor_loss	1.0000
7:149432933:C:CC	acceptor_gain	1.0000
7:149432933:C:G	acceptor_gain	1.0000
7:149432939:C:CT	acceptor_gain	1.0000
7:149432940:A:T	acceptor_gain	1.0000
7:149432945:C:CT	acceptor_gain	1.0000
7:149436571:TCA:T	donor_loss	1.0000
7:149436572:CACC:C	donor_loss	1.0000
7:149436573:A:AC	donor_gain	1.0000
7:149436573:AC:A	donor_gain	1.0000
7:149436574:C:CA	donor_loss	1.0000
7:149436574:C:CC	donor_gain	1.0000
7:149436574:CC:C	donor_gain	1.0000
7:149436827:C:CC	acceptor_gain	1.0000
7:149436847:G:C	acceptor_gain	1.0000
7:149450991:CCACT:C	donor_loss	1.0000
7:149450992:CACTC:C	donor_loss	1.0000
7:149450993:ACTCA:A	donor_loss	1.0000
7:149450994:CTCAC:C	donor_loss	1.0000
7:149450995:T:TA	donor_loss	1.0000
7:149450996:CACCA:C	donor_loss	1.0000
7:149450997:A:AC	donor_gain	1.0000
7:149450998:C:CC	donor_gain	1.0000
7:149450998:CCAG:C	donor_gain	1.0000
7:149454105:CCTTA:C	donor_loss	1.0000
7:149454106:CTTA:C	donor_loss	1.0000
7:149454107:TTACC:T	donor_loss	1.0000
7:149454108:TA:T	donor_loss	1.0000

AlphaMissense

5459 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:149431827:G:C	F815L	1.000
7:149431827:G:T	F815L	1.000
7:149431828:A:G	F815S	1.000
7:149431829:A:G	F815L	1.000
7:149431911:G:C	F787L	1.000
7:149431911:G:T	F787L	1.000
7:149431912:A:G	F787S	1.000
7:149431913:A:G	F787L	1.000
7:149431970:G:C	H768D	1.000
7:149431978:A:G	L765P	1.000
7:149431995:G:C	F759L	1.000
7:149431995:G:T	F759L	1.000
7:149431996:A:G	F759S	1.000
7:149431997:A:G	F759L	1.000
7:149432018:A:G	C752R	1.000
7:149432052:G:C	H740Q	1.000
7:149432052:G:T	H740Q	1.000
7:149432054:G:C	H740D	1.000
7:149432062:A:G	L737P	1.000
7:149432079:G:C	F731L	1.000
7:149432079:G:T	F731L	1.000
7:149432080:A:G	F731S	1.000
7:149432081:A:G	F731L	1.000
7:149432102:A:G	C724R	1.000
7:149432146:A:G	L709P	1.000
7:149432163:G:C	F703L	1.000
7:149432163:G:T	F703L	1.000
7:149432165:A:G	F703L	1.000
7:149432253:G:C	F673L	1.000
7:149432253:G:T	F673L	1.000

dbSNP variants (sampled 300 via entrez): RS1000102341 (7:149457301 C>A,T), RS1000121165 (7:149433490 G>A,C), RS1000280897 (7:149439871 C>A), RS1000355940 (7:149435858 G>A), RS1000382418 (7:149446379 A>C), RS1000464735 (7:149439430 G>A,C,T), RS1000505217 (7:149435098 T>A,C), RS1000513012 (7:149462048 G>C), RS1000804308 (7:149440669 T>A,G), RS1001059736 (7:149446659 T>A,C,G), RS1001098099 (7:149456773 T>C), RS1001108040 (7:149440434 A>C,G), RS1001137641 (7:149439103 A>G), RS1001276277 (7:149462505 T>C), RS1001394171 (7:149432807 T>C,G)

Disease associations

OMIM: gene MIM:619298 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

26 total (human), top 26 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
GSK-J4	increases expression	1
TAK-243	decreases sumoylation	1
dicrotophos	increases expression	1
deoxynivalenol	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
sodium arsenite	increases expression	1
di-n-butylphosphoric acid	affects expression	1
cylindrospermopsin	increases expression	1
ICG 001	increases expression	1
abrine	increases expression	1
2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazophenyl)amide	affects cotreatment, decreases expression	1
Sunitinib	increases expression	1
Arsenic	affects methylation	1
Atrazine	decreases expression	1
Benzo(a)pyrene	decreases methylation	1
Caffeine	decreases phosphorylation	1
Doxorubicin	decreases expression	1
Lead	decreases expression	1
Phenobarbital	affects expression	1
Quercetin	increases phosphorylation	1
Tetrachlorodibenzodioxin	affects cotreatment, decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Valproic Acid	increases methylation	1
Cyclosporine	increases expression	1
Sodium Selenite	increases expression	1
Cadmium Chloride	decreases expression	1

Cellosaurus cell lines

3 cell lines: 2 cancer cell line, 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_TZ48	HAP1 ZNF777 (-) 1	Cancer cell line	Male
CVCL_TZ49	HAP1 ZNF777 (-) 2	Cancer cell line	Male
CVCL_XW84	HEK293 eGFP-ZNF777	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.