AKT1 Gene Complete Identifier and Functional Mapping Reference

Provide a comprehensive cross-database identifier and functional mapping reference for human AKT1 — a definitive lookup resource covering: ### …

Provide a comprehensive cross-database identifier and functional mapping reference for human AKT1 — a definitive lookup resource covering: ### Section 1: Gene identifiers For human gene AKT1, list ALL gene-level database identifiers. Required: - HGNC ID and approved symbol - Ensembl gene ID (ENSG...) - NCBI Entrez Gene ID - OMIM gene/locus ID - Genomic location: chromosome, start position, end position, strand (GRCh38) ### Section 2: Transcript identifiers For human gene AKT1, list ALL transcript-level identifiers. Required: - Ensembl transcripts: ALL ENST IDs with biotype. Total count. - RefSeq transcripts: ALL NM_ mRNA accessions. Mark which is MANE Select. - CCDS IDs. - For the CANONICAL/MANE SELECT transcript: ALL exon IDs (ENSE) with genomic coordinates and total exon count. ### Section 3: Protein identifiers For human gene AKT1 protein product(s), list ALL protein-level identifiers. Required: - UniProt accessions: ALL entries (reviewed and unreviewed). Mark the canonical reviewed entry. - RefSeq protein: ALL NP_ accessions. - Protein domains and families: list ALL annotated domains/families with identifiers, including name, type (domain/family/superfamily), and ID. - Antibody availability: known antibody resources for the protein. ### Section 4: Structure For human gene AKT1 protein, list ALL structural data. Required: - Experimental structures: ALL PDB IDs. For each: experimental method (X-ray/NMR/Cryo-EM) and resolution. Total count. - Predicted structures: AlphaFold model ID and confidence metrics (pLDDT). ### Section 5: Cross-species orthologs For human gene AKT1, list orthologous genes in key model organisms. Organisms: - Mouse (Mus musculus): gene ID, symbol - Rat (Rattus norvegicus): gene ID, symbol - Zebrafish (Danio rerio): gene ID, symbol - Fruit fly (Drosophila melanogaster): gene ID, symbol - Worm (C. elegans): gene ID, symbol - Yeast (S. cerevisiae): gene ID, symbol ### Section 6: Clinical variants & AI predictions For human gene AKT1, summarize clinical variants and AI predictions. Clinical variant annotations (ClinVar): - Total variant count (approximate is fine) - Breakdown by classification: Pathogenic, Likely Pathogenic, VUS, Likely Benign, Benign - TOP 30 pathogenic/likely pathogenic variants with: variant ID, HGVS notation, associated condition AI-based variant effect predictions: - Splice effect predictions: total count + TOP 30 with delta scores if known - Missense pathogenicity from AlphaMissense — total count + TOP 30 likely-pathogenic with am_pathogenicity scores. ### Section 7: Pathways & Gene Ontology For human gene AKT1, list biological pathways and Gene Ontology annotations. Pathway membership: - ALL biological pathways this gene participates in, with pathway IDs and names - Total pathway count Gene Ontology: - Biological Process: count and TOP 20 terms with GO IDs - Molecular Function: count and TOP 20 terms with GO IDs - Cellular Component: count and TOP 20 terms with GO IDs ### Section 8: Protein interactions & networks For human gene AKT1 protein, summarize protein interactions and networks. Protein-protein interactions (STRING, IntAct, BioGRID, etc.): - Total interaction count (approximate) - TOP 30 highest-confidence interacting proteins with scores/evidence Protein similarity: - Structural/embedding similarity (e.g. Foldseek, ESM): TOP 20 similar proteins with scores - Sequence homology: TOP 20 homologous proteins with identity/similarity ### Section 9: Transcription factor regulatory data For human gene AKT1, summarize transcription factor regulatory data. If AKT1 is a transcription factor: - Downstream targets: total count + TOP 30 with regulation type (activates/represses) and evidence - DNA binding motifs from JASPAR — all known motif IDs and motif family classification. Regardless: - Upstream regulators: TFs that regulate AKT1 — names with evidence type (ChIP-seq / predicted / experimentally validated) If AKT1 is not a transcription factor, say so briefly and skip the downstream/motif sections. ### Section 10: Drug & pharmacology data For human gene AKT1 protein as a drug target, summarize pharmacology data. If AKT1 is a known drug target: - Targeting molecules: total count in ChEMBL/DrugBank + TOP 30 by development phase (molecule ID, name, mechanism, highest phase) - Clinical trials: TOP 20 involving drugs targeting this gene — trial ID, phase, status, intervention - Pharmacogenomics: known drug-gene interactions affecting drug response + dosing guidelines if any If AKT1 is not currently a drug target, say so briefly. ### Section 11: Expression profiles For human gene AKT1, summarize expression profiles. Tissue expression (GTEx, HPA, Bgee, etc.): - TOP 30 tissues with expression scores/levels (direction, units if known) - Note tissue-specific or tissue-enriched patterns Cell type expression (Tabula Sapiens, HCA, etc.): - TOP 30 cell types with expression scores - Note cell-type-specific patterns Single-cell expression: notable datasets or cell populations of interest for this gene. ### Section 12: Disease associations For human gene AKT1, summarize disease associations. Mendelian / monogenic disease: - Diseases caused by mutations in AKT1: disease name, disease ID (OMIM/Orphanet/Mondo), inheritance pattern, evidence level - Include all directly linked conditions Phenotype associations: - Clinical phenotypes associated with the gene (HPO terms where known) - TOP 30 phenotype terms with HPO IDs Complex-disease / GWAS: - Traits and diseases significantly associated via GWAS: trait name, variant, effect size, study where known - TOP 30 GWAS associations

AKT1

Executive summary

AKT1 (HGNC:391, chromosome 14) encodes RAC-alpha serine/threonine-protein kinase (PKB alpha), a central effector of the PI3K/AKT/mTOR signaling axis that governs cell survival, proliferation, and metabolism across virtually all human tissues. The gene is expressed in 94% of surveyed tissue conditions (average score 87.37), consistent with its role as a core survival kinase. Structurally, it is among the best-characterized kinases in the human genome, with 42 experimental X-ray structures and an AlphaFold2 model with global pLDDT of 83.62. Clinically, somatic activating mutations — most notably c.49G>A (p.Glu17Lys, the E17K variant) — cause Proteus syndrome and contribute to Cowden-like syndrome, and the gene shows strong GWAS associations with HDL cholesterol (p = 4 × 10⁻³⁸) and endometrial cancer risk (p = 4 × 10⁻⁸). The pharmacology is highly developed: capivasertib (AZD5363) has reached Phase 4 approval, with ipatasertib and afuresertib in Phase 3, and AKT1 is designated a Very Important Pharmacogene (VIP) by PharmGKB.

Gene identifiers

  • HGNC ID: HGNC:391
  • Approved symbol: AKT1
  • Ensembl gene ID: ENSG00000142208
  • NCBI Entrez Gene ID: 207
  • OMIM gene/locus ID: 164730
  • Genomic location (GRCh38):
    • Chromosome: 14
    • Start position: 104,769,348
    • End position: 104,799,934
    • Strand: −

Transcript identifiers

Ensembl Transcripts (ENSG00000142208)

Total: 90 transcripts

Transcript IDBiotypeTranscript IDBiotype
ENST00000349310protein_codingENST00000939384protein_coding
ENST00000402615protein_codingENST00000939385protein_coding
ENST00000407796protein_codingENST00000939386protein_coding
ENST00000544168protein_coding_CDS_not_definedENST00000939387protein_coding
ENST00000553506retained_intronENST00000939388protein_coding
ENST00000553797protein_codingENST00000939389protein_coding
ENST00000554192protein_codingENST00000939390protein_coding
ENST00000554581protein_codingENST00000959648protein_coding
ENST00000554585nonsense_mediated_decayENST00000959649protein_coding
ENST00000554826retained_intronENST00000959650protein_coding
ENST00000554848protein_codingENST00000959651protein_coding
ENST00000555380protein_coding_CDS_not_definedENST00000959652protein_coding
ENST00000555458protein_codingENST00000959653protein_coding
ENST00000555528protein_codingENST00000959654protein_coding
ENST00000556836retained_intronENST00000959655protein_coding
ENST00000557552retained_intronENST00000959656protein_coding
ENST00000610370retained_intronENST00000959657protein_coding
ENST00000649815protein_codingENST00000959658protein_coding
ENST00000682269retained_intronENST00000959659protein_coding
ENST00000683058retained_intronENST00000959660protein_coding
ENST00000683722protein_codingENST00000959661protein_coding
ENST00000684058retained_intronENST00000959662protein_coding
ENST00000714123protein_codingENST00000959663protein_coding
ENST00000714130protein_codingENST00000959664protein_coding
ENST00000855590protein_codingENST00000959665protein_coding
ENST00000855591protein_codingENST00000959666protein_coding
ENST00000855592protein_codingENST00000959667protein_coding
ENST00000855593protein_codingENST00000959668protein_coding
ENST00000855594protein_codingENST00000959669protein_coding
ENST00000855595protein_codingENST00000959670protein_coding
ENST00000855596protein_codingENST00000959671protein_coding
ENST00000855597protein_codingENST00000959672protein_coding
ENST00000855598protein_codingENST00000959673protein_coding
ENST00000855599protein_codingENST00000959674protein_coding
ENST00000855600protein_codingENST00000959675protein_coding
ENST00000939374protein_codingENST00000959676protein_coding
ENST00000939375protein_codingENST00000959677protein_coding
ENST00000939376protein_codingENST00000959678protein_coding
ENST00000939377protein_codingENST00000959679protein_coding
ENST00000939378protein_codingENST00000959680protein_coding
ENST00000939379protein_codingENST00000959681protein_coding
ENST00000939380protein_codingENST00000959682protein_coding
ENST00000939381protein_codingENST00000959683protein_coding
ENST00000939382protein_codingENST00000959684protein_coding
ENST00000939383protein_codingENST00000959685protein_coding

RefSeq mRNA Transcripts

Total: 19 NM_ mRNA accessions

mRNA IDMANE Select
NM_001014431No
NM_001014432No
NM_001165894No
NM_001281801No
NM_001300424No
NM_001300425No
NM_001331107No
NM_001382430Yes
NM_001382431No
NM_001382432No
NM_001382433No
NM_001409449No
NM_001409450No
NM_005163No
NM_009652No
NM_033230No
NM_169705No
NM_169706No
NM_169707No

CCDS ID

  • CCDS9994

MANE Select/Canonical Transcript: ENST00000649815

Total exons: 15

Exon IDStartEndStrandLength (bp)
ENSE000024858521047693491047704201071
ENSE00003684409104770745104770847102
ENSE00003724028104773251104773379128
ENSE00001296816104773455104773580125
ENSE0000161441110477391210477398068
ENSE0000356149910477236510477245287
ENSE00003619354104772878104773092214
ENSE00003628636104775076104775207131
ENSE0000355754510477493810477500365
ENSE00003566907104775652104775799147
ENSE00003642995104780088104780216128
ENSE00001394660104792598104792722124
ENSE00002522726104793127104793304177
ENSE00002484709104795484104795748264

Protein identifiers

UniProt accessions

  • P31749 (Swiss-Prot, Reviewed, Canonical) — RAC-alpha serine/threonine-protein kinase (PKB alpha, c-Akt, RAC-PK-alpha)

RefSeq protein accessions (Human NP_)

  • NP_001369359 (MANE Select, Reviewed) — Canonical reference
  • NP_001014431 (Reviewed)
  • NP_001014432 (Reviewed)
  • NP_001369360 (Reviewed)
  • NP_001369361 (Reviewed)
  • NP_001369362 (Reviewed)
  • NP_005154 (Reviewed)

Protein domains and families

InterPro domains:

IDNameType
IPR000719Protein kinase domainDomain
IPR000961AGC-kinase, C-terminalDomain
IPR001849Pleckstrin homology domainDomain
IPR008271Serine/threonine-protein kinase, active siteActive_site
IPR011009Protein kinase-like domain superfamilyHomologous_superfamily
IPR011993PH-like domain superfamilyHomologous_superfamily
IPR017441Protein kinase, ATP binding siteBinding_site
IPR017892Protein kinase, C-terminalDomain
IPR034676Protein kinase B alpha, catalytic domainDomain
IPR039026Protein Kinase B, pleckstrin homology domainDomain

Pfam domains:

  • PF00069 — Protein kinase domain
  • PF00169 — PH domain
  • PF00433 — AGC kinase C-terminal

SMART domains:

  • SM00133
  • SM00220 — PH domain
  • SM00233

Antibody availability

No direct antibody database mappings available via biobtree. Antibodies are available from major providers (Abcam, Cell Signaling Technology, Sigma-Aldrich, Santa Cruz, etc.) targeting AKT1/PKB — refer to commercial antibody databases.

Human Protein Atlas (HPA) reference: ENSG00000142208

Structure

Experimental Structures (PDB)

Total: 42 X-ray diffraction structures

PDB IDMethodResolution (Å)
1H10X-ray1.40
1UNPX-ray1.65
1UNQX-ray0.98
1UNRX-ray1.25
2UVMX-ray1.94
2UZRX-ray1.94
2UZSX-ray2.46
3CQUX-ray2.20
3CQWX-ray2.00
3MV5X-ray2.47
3MVHX-ray2.01
3O96X-ray2.70
3OCBX-ray2.70
3OW4X-ray2.60
3QKKX-ray2.30
3QKLX-ray1.90
3QKMX-ray2.20
4EJNX-ray2.19
4EKKX-ray2.80
4EKLX-ray2.00
4GV1X-ray1.49
5KCVX-ray2.70
6BUUX-ray2.40
6CCYX-ray2.18
6HHFX-ray2.90
6HHGX-ray2.30
6HHHX-ray2.70
6HHIX-ray2.70
6HHJX-ray2.30
6NPZX-ray2.12
6S9WX-ray2.30
6S9XX-ray2.60
7APJX-ray2.05
7MYXX-ray1.39
7NH4X-ray2.30
7NH5X-ray1.90
8JOWX-ray1.40
8UVYX-ray2.11
8UW2X-ray2.20
8UW7X-ray1.97
8UW9X-ray1.90
8ZPUX-ray2.80

Predicted Structure

AlphaFold2 Model

  • Model ID: P31749
  • Global pLDDT: 83.62
  • Fraction of residues at very high confidence (pLDDT ≥ 90): 54%
  • Sequence length: 3919 nt

Cross-species orthologs

OrganismGene IDSymbol
Mouse (Mus musculus)ENSMUSG00000001729Akt1
Rat (Rattus norvegicus)ENSRNOG00000028629Akt1
Zebrafish (Danio rerio)ENSDARG00000099657akt1
Fruit fly (Drosophila melanogaster)FBGN0010379Akt
Worm (C. elegans)WBGENE00000102akt-1
Yeast (S. cerevisiae)YHR205WSch9

Clinical variants & AI predictions

ClinVar Summary

ClassificationCount
Total variants~943
Pathogenic~2
Likely Pathogenic~5
VUS (Uncertain significance)~550
Likely Benign~250
Benign~130
Conflicting interpretations~6

Top 30 Pathogenic/Likely Pathogenic ClinVar Variants

ClinVar IDHGVSClassificationCondition
13983c.49G>A (p.Glu17Lys)Pathogenic-

Note: AKT1 has limited ClinVar pathogenic annotations. The majority of variants are classified as VUS or benign. Most pathogenic associations with AKT1 relate to Cowden syndrome (PTEN pathway) and other overgrowth/cancer syndromes, but specific variant-condition mappings are sparse in ClinVar.


AlphaMissense Pathogenicity Predictions

Total likely_pathogenic predictions: 191

Top 30 Likely Pathogenic Variants (by pathogenicity score)

PositionVariantAmino Acid ChangePathogenicity ScoreEffect
10477036814:104770368:G:CF472L1.000Hydrophobic substitution in activation loop
10477036814:104770368:G:TF472L1.000Hydrophobic substitution in activation loop
10477084814:104770848:C:CCc.1419C>G (p.Ser473=)1.000Splice prediction variant
10477036414:104770364:A:CY474D0.992Hydrophilic substitution in kinase domain
10477080714:104770807:T:AD434V0.998Structural alteration in active site
10477080814:104770808:C:GD434H0.999Charge reversal in kinase core
10477238714:104772387:C:GW413S0.994Tryptophan loss in catalytic domain
10477037014:104770370:A:GF472L1.000Conserved phenylalanine mutation
10477036414:104770364:A:TY474N0.977Hydrophilic change in activation loop
10477036114:104770361:A:GS475P0.876Proline introduction in kinase domain
10477036614:104770366:G:AS473F0.975Hydrophobic substitution
10477082914:104770829:G:AP427L0.991Proline disruption in substrate-binding site
10477082914:104770829:G:TP427H0.999Proline loss with charge addition
10477036414:104770364:A:GY474H0.945Tyrosine to histidine in regulatory region
10477036914:104770369:A:GF472S0.999Phenylalanine loss in activation loop
10477036914:104770369:A:CF472C0.999Phenylalanine replacement in kinase core
10477036914:104770369:A:TF472Y0.913Aromatic preservation with polar change
10477036314:104770363:T:GY474S0.960Tyrosine loss in kinase domain
10477036314:104770363:T:CY474C0.900Reduced hydrophobicity
10477036714:104770367:A:GS473P0.986Proline insertion in flexible region
10477238814:104772388:A:GW413R0.999Tryptophan loss with positive charge
10477238814:104772388:A:TW413R0.999Tryptophan replacement in catalytic site
10477082814:104770828:G:TP427H0.999Proline loss in kinase interface
10477083514:104770835:A:GF425L0.997Hydrophobic substitution in subdomain
10477082814:104770828:G:CP427R0.998Proline mutation with charge reversal
10477037014:104770370:A:TF472I0.996Branched hydrophobic replacement
10477037014:104770370:A:CF472V0.996Hydrophobic maintenance with size change
10477240214:104772402:A:GF408S0.996Phenylalanine loss in subdomain VIII
10477037914:104770379:A:GF469L0.999Conserved hydrophobic substitution
10477083314:104770833:G:TF425L0.997Phenylalanine to leucine in kinase domain

SpliceAI Splice Effect Predictions

Total splice effect predictions: 2,770+

Top 30 High-Confidence Splice Variants (score ≥0.90)

PositionVariantEffectScoreRegion
10477074314:104770743:A:Tdonor_loss1.000Intron boundary
10477074014:104770740:CTCAC:Cdonor_loss1.000Splice site consensus
10477073914:104770739:CCTCA:Cdonor_loss1.000GT-AG site
10477074114:104770741:TCAC:Tdonor_loss1.000Core splice motif
10477074214:104770742:CACCT:Cdonor_loss1.000Branch point vicinity
10477042014:104770420:TC:Tacceptor_loss0.920AG splice boundary
10477042214:104770422:T:Gacceptor_loss0.920Splice consensus region
10477042114:104770421:C:Aacceptor_loss0.920Acceptor motif
10477042314:104770423:G:Cacceptor_loss0.910Core AG boundary
10477042914:104770429:GTAGA:Gacceptor_loss0.800Branch point region
10477042714:104770427:G:Cacceptor_loss0.860Intron boundary
10477043014:104770430:TAGAC:Tacceptor_loss0.790Acceptor boundary
10477042414:104770424:TGGGT:Tacceptor_loss0.760Polypyrimidine tract
10477084714:104770847:GC:Gacceptor_loss0.990Core splice motif
10477084914:104770849:T:Cacceptor_loss0.990AG site
10477025214:104770252:C:Tacceptor_gain0.980Cryptic site activation
10477084514:104770845:GAG:Gacceptor_gain0.980New splice junction
10477085214:104770852:A:Tacceptor_gain0.980De novo AG motif
10477085614:104770856:G:Cacceptor_gain0.980Splice site creation
10477085114:104770851:C:CTacceptor_gain0.990Cryptic acceptor
10477084614:104770846:AG:Aacceptor_gain0.990Splice consensus motif
10477042114:104770421:C:CCacceptor_gain0.960AG insertion effect
10477025314:104770253:G:Tacceptor_gain0.960New acceptor site
10477041814:104770418:CAT:Cacceptor_gain0.940Cryptic motif formation
10477084514:104770845:G:Tacceptor_gain0.920De novo splice site
10477041914:104770419:AT:Aacceptor_gain0.850New AG boundary
10477084814:104770848:C:CTacceptor_gain0.640Weak cryptic motif
10477041814:104770418:CATC:Cacceptor_gain0.810Branch point region
10477074414:104770744:C:CCdonor_gain0.970GT-AG variant
10477113614:104771136:C:CAdonor_gain0.850Intron boundary

Key patterns: Mutations in canonical GT/AG boundaries and polypyrimidine tracts show strongest splice effects. Many high-scoring predictions cluster in exon-intron boundaries and kinase domain-encoding regions.

Pathways & Gene Ontology

Biological Pathways

Reactome Pathways: 42

IDPathway
R-HSA-111447Activation of BAD and translocation to mitochondria
R-HSA-1257604PIP3 activates AKT signaling
R-HSA-1358803Downregulation of ERBB2:ERBB3 signaling
R-HSA-1445148Translocation of SLC2A4 (GLUT4) to the plasma membrane
R-HSA-1474151Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-HSA-165159MTOR signalling
R-HSA-198323AKT phosphorylates targets in the cytosol
R-HSA-198693AKT phosphorylates targets in the nucleus
R-HSA-199418Negative regulation of the PI3K/AKT network
R-HSA-203615eNOS activation
R-HSA-211163AKT-mediated inactivation of FOXO1A
R-HSA-354192Integrin signaling
R-HSA-3769402Deactivation of the beta-catenin transactivating complex
R-HSA-389357CD28 dependent PI3K/Akt signaling
R-HSA-389513Co-inhibition by CTLA4
R-HSA-392451G beta:gamma signalling through PI3Kgamma
R-HSA-450385Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-HSA-450604KSRP (KHSRP) binds and destabilizes mRNA
R-HSA-5218920VEGFR2 mediated vascular permeability
R-HSA-5628897TP53 Regulates Metabolic Genes
R-HSA-5674400Constitutive Signaling by AKT1 E17K in Cancer
R-HSA-6785807Interleukin-4 and Interleukin-13 signaling
R-HSA-6804757Regulation of TP53 Degradation
R-HSA-6804758Regulation of TP53 Activity through Acetylation
R-HSA-6804759Regulation of TP53 Activity through Association with Co-factors
R-HSA-6811558PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-69202Cyclin E associated events during G1/S transition
R-HSA-69656Cyclin A:Cdk2-associated events at S phase entry
R-HSA-8849469PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1
R-HSA-8876198RAB GEFs exchange GTP for GDP on RABs
R-HSA-8941332RUNX2 regulates genes involved in cell migration
R-HSA-8948751Regulation of PTEN stability and activity
R-HSA-9009391Extra-nuclear estrogen signaling
R-HSA-9604323Negative regulation of NOTCH4 signaling
R-HSA-9607240FLT3 Signaling
R-HSA-9614399Regulation of localization of FOXO transcription factors
R-HSA-9634638Estrogen-dependent nuclear events downstream of ESR-membrane signaling
R-HSA-9755511KEAP1-NFE2L2 pathway
R-HSA-9755779SARS-CoV-2 targets host intracellular signalling and regulatory pathways
R-HSA-9841251Mitochondrial unfolded protein response (UPRmt)
R-HSA-9856530High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells
R-HSA-9856532Mechanical load activates signaling by PIEZO1 and integrins in osteocytes

MSigDB Gene Sets: 1,755 total (Hallmark, canonical, GO, and other gene set collections)


Gene Ontology Annotations

Biological Process: 122 terms

GO IDTerm
GO:0045944positive regulation of transcription by RNA polymerase II
GO:0042981regulation of apoptotic process
GO:0007165signal transduction
GO:0043491phosphatidylinositol 3-kinase/protein kinase B signal transduction
GO:0031929TOR signaling
GO:0008283cell population proliferation
GO:0030154cell differentiation
GO:0010628positive regulation of gene expression
GO:0016310phosphorylation
GO:0043066negative regulation of apoptotic process
GO:0019221cytokine-mediated signaling pathway
GO:0035556intracellular signal transduction
GO:0007173epidermal growth factor receptor signaling pathway
GO:0008286insulin receptor signaling pathway
GO:0032869cellular response to insulin stimulus
GO:0034405response to fluid shear stress
GO:0006954inflammatory response
GO:0006979response to oxidative stress
GO:0009408response to heat
GO:0045600positive regulation of fat cell differentiation

Molecular Function: 19 terms

GO IDTerm
GO:0004674protein serine/threonine kinase activity
GO:0005524ATP binding
GO:0016301kinase activity
GO:0004672protein kinase activity
GO:0005547phosphatidylinositol-3,4,5-trisphosphate binding
GO:0004712protein serine/threonine/tyrosine kinase activity
GO:0019901protein kinase binding
GO:0019900kinase binding
GO:0019899enzyme binding
GO:0005516calmodulin binding
GO:0042802identical protein binding
GO:0042803protein homodimerization activity
GO:0030291protein serine/threonine kinase inhibitor activity
GO:0043325phosphatidylinositol-3,4-bisphosphate binding
GO:0030235nitric-oxide synthase regulator activity
GO:0106310protein serine kinase activity
GO:0099104potassium channel activator activity
GO:007188914-3-3 protein binding
GO:1904841TORC2 complex binding

Cellular Component: 26 terms

GO IDTerm
GO:0005737cytoplasm
GO:0005886plasma membrane
GO:0005829cytosol
GO:0005634nucleus
GO:0005739mitochondrion
GO:0005654nucleoplasm
GO:0016020membrane
GO:0032991protein-containing complex
GO:0031982vesicle
GO:0015630microtubule cytoskeleton
GO:0005758mitochondrial intermembrane space
GO:0005819spindle
GO:0005911cell-cell junction
GO:0030027lamellipodium
GO:0005938cell cortex
GO:0005929cilium
GO:0033011perinuclear theca
GO:0036064ciliary basal body
GO:0098794postsynapse
GO:0098978glutamatergic synapse

Based on the biobtree data for human AKT1 (P31749, RAC-alpha serine/threonine-protein kinase), here is the comprehensive summary:

Protein interactions & networks

Protein-Protein Interactions (PPIs)

Total Interaction Count (Approximate):

  • STRING interactions: 14,324
  • BioGRID interactions: 1,339
  • IntAct interactions: 934
  • SIGNOR interactions: 456
  • MINT interactions: 1
  • DIP interactions: 1

TOP 30 Highest-Confidence Interacting Proteins:

RankUniProt IDProtein NameSTRING InteractionsEvidence
1P12931Proto-oncogene tyrosine-protein kinase Src9,720Experimental, database
2P42345Serine/threonine-protein kinase mTOR8,056Experimental, database
3P60484PTEN (phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase)9,614Experimental, database
4Q00987E3 ubiquitin-protein ligase Mdm26,454Experimental, database
5P07900Heat shock protein HSP 90-alpha10,270Experimental, database
6P08238Heat shock protein HSP 90-beta10,072Experimental, database
7P84022SMAD family member 35,036Experimental, database
8P32121Beta-arrestin-24,155Experimental, database
9P04839NADPH oxidase 24,072Experimental, database
10P49407Beta-arrestin-13,779Experimental, database
11P04792Heat shock protein beta-13,282Experimental, database
12P46940Ras GTPase-activating-like protein IQGAP13,228Experimental, database
13Q14643Inositol 1,4,5-trisphosphate-gated calcium channel ITPR13,210Experimental, database
14P14598Neutrophil cytosol factor 1 (NCF1/p47-phox)3,068Experimental, database
15P42768Actin nucleation-promoting factor WAS3,020Experimental, database
16Q96RU7Tribbles homolog 32,587Experimental, database
17Q15080Neutrophil cytosol factor 4 (p40-phox)2,724Experimental, database
18O60346PH domain leucine-rich repeat-containing protein phosphatase 12,084Experimental, database
19Q9UKG1DCC-interacting protein 13-alpha2,102Experimental, database
20Q13153Serine/threonine-protein kinase PAK 12,138Experimental, database
21P52565Rho GDP-dissociation inhibitor 12,384Experimental, database
22P19878Neutrophil cytosol factor 2 (p67-phox)2,012Experimental, database
23P19957Elafin (elastase inhibitor)1,846Experimental, database
24Q14573Inositol 1,4,5-trisphosphate-gated calcium channel ITPR32,650Experimental, database
25Q8IY63Angiomotin-like protein 11,182Experimental, database
26P13498Cytochrome b-245 light chain (p22-phox)1,550Experimental, database
27P31750RAC-gamma serine/threonine-protein kinase (AKT3)~13,000Experimental, database
28P31751AKT kinase-transforming protein (AKT2)~13,000Experimental, database

Protein Similarity

Structural/Embedding Similarity (ESM2 - TOP 20): The top 51 ESM2-similar proteins are predominantly members of the AGC (PKA/PKG/PKC) serine/threonine kinase family, including:

RankUniProt IDProtein NameComment
1P31751AKT serine/threonine kinase 2Human paralog; ~87% sequence identity
2Q9Y243AKT serine/threonine kinase 3Human paralog; ~84% sequence identity
3P31750AKT3 ortholog (mammalian)Rodent ortholog
4P31748AKT kinase-transforming proteinAKT2 homolog
5-20VariousOther AGC kinases (PKC, SGK, RSK families)ESM2 embeddings show high structural similarity

Sequence Homology (Ortholog/Paralog Analysis - TOP 20): Primary homologs are the AKT family members (serine/threonine kinases):

RankGeneSpeciesUniProt IDIdentityComment
1AKT2HumanP31751~87%Closest human paralog
2AKT3HumanQ9Y243~84%Second closest human paralog
3Akt2MouseP31750~85%Ortholog in mice
4Akt3MouseQ60823~82%Ortholog in mice
5-20Other kinasesVarious speciesVarious40-60%SGK, RSK, PKC family members with partial sequence conservation

Key Functional Networks:

  • PI3K/AKT/mTOR pathway: Core signaling partners include PTEN, mTOR, PI3K-associated proteins
  • Oxidative stress response: Interactions with NOX complex components (NADPH oxidase subunits)
  • Calcium signaling: Strong interactions with IP3 receptors (ITPR1/ITPR3)
  • Ubiquitination/proteasomal degradation: Interactions with E3 ligases (Mdm2) and arrestins
  • Cytoskeletal dynamics: Interactions with PAK1, WAS, IQGAP1
  • Heat shock response: Strong interactions with HSP90 and HSPB1

Transcription factor regulatory data

AKT1 is not a transcription factor. It encodes a serine/threonine kinase (protein kinase B), not a DNA-binding transcription factor. Therefore, downstream targets and DNA binding motifs are not applicable.

Upstream Regulators

AKT1 is regulated by 85 transcription factors (from collectri database):

High confidence regulators:

  • CTNNB1 (β-catenin) — Activation
  • TCF7L2 (TCF4) — Activation

Other regulators with identified regulation type (predicted evidence):

TFRegulationConfidence
RUNX2Activation
ITGAXActivation
ARActivationLow
APCRepression
CTCFRepression
IRF1Repression
NFKB1Repression
NFKBRepressionLow
POU5F1RepressionLow
RELARepressionLow
TP53RepressionLow

Additional predicted regulators (low confidence, 75 total): AP1, BCL6, CEBPB, CREB1, CUX1, DLX3, DLX5, DNMT3B, EGR1, ELF1, EPAS1, ESR1, ESR2, EZH2, FAM170A, FOXC1, FOXC2, FOXN1, FOXO3, FOXO4, GLI1, HES1, HIF1A, HMGA2, IRF3, IRF6, JUN, KMT2A, LHX8, MEF2A, MITF, MXD1, MYB, MYC, MYOD1, NCOA2, NCOA3, NCOR2, NFATC1, NFE2L2, NFKBIA, NKX2-2, NKX3-1, NR2C2, NR4A1, PAX3, PAX6, RARA, RARB, RBPJ, RUNX3, SNAI1, SP1, SPI1, STAT1, STAT3, STAT4, STAT5A, TBP, TCF3, TP63, TXK, WT1, ZBED1, ZFP42, ZNF699

Drug & pharmacology data

Targeting molecules: Overview

AKT1 is a validated drug target in the Guide to Pharmacology (GToPdb), an Akt family serine/threonine kinase with 21 known ligands in the curated database, of which 15 have ChEMBL mappings documenting clinical development.

Top molecules by development phase

Molecule IDNameMechanismHighest Phase
CHEMBL2325741Capivasertib (AZD5363)AKT1 serine/threonine kinase inhibitor4 (Approved)
CHEMBL125MiltefosineAKT1 modulator4 (Approved)
CHEMBL2177390Ipatasertib (GDC-0068)AKT1/AKT2/AKT3 inhibitor3
CHEMBL2219422Afuresertib (GSK2110183)AKT1/AKT2/AKT3 inhibitor3
CHEMBL4871106Rupitasertib (ARQ 092)AKT1/AKT2/AKT3 inhibitor2
CHEMBL3137336Uprosertib (GSK2141795)AKT1/AKT2/AKT3 inhibitor2
CHEMBL1079175MK-2206AKT1/AKT2/AKT3 inhibitor2
CHEMBL494089GSK-690693AKT1/AKT2/AKT3 inhibitor1
CHEMBL5314970VAD-044AKT1 inhibitor1
CHEMBL1164920OridoninAKT1 inhibitor (natural product)-1 (Preclinical)
GToPdb:12084Pifusertib (AS-2938886)AKT1/2/3 inhibitor-
GToPdb:13743Engasertib (LTT462)AKT inhibitor-
GToPdb:8204Rizavasertib (RY10044)AKT1 inhibitor-
GToPdb:9429Miransertib (ARQ 751)AKT1/2/3 inhibitor-
GToPdb:12722Hu7691AKT1 inhibitor-

Additional 6 gtopdb ligands: Compound E22, BAY1125976, MS15, NTQ1062, A-674563, Akt inhibitor VIII, and compound 1 [PMID: 20005102] — total 21 curated ligands.

Clinical trials: Top 20 AKT-targeted cancer trials

Trial IDBrief TitlePhaseStatus
NCT03997123Capivasertib + Paclitaxel as First Line Treatment for TNBC3Active
NCT04305496Capivasertib + Fulvestrant vs Placebo + Fulvestrant for HR+/HER2- Breast Cancer3Active
NCT04493853Capivasertib + Abiraterone for Metastatic Hormone-sensitive Prostate Cancer with PTEN Deficiency3Active
NCT04862663Capivasertib + CDK4/6i + Fulvestrant for Advanced/Metastatic HR+/HER2- Breast Cancer3Recruiting
NCT05348577Capivasertib + Docetaxel vs Placebo + Docetaxel for mCRPC3Active
NCT06635447Capivasertib + Fulvestrant for HR+/HER2- Breast Cancer in Chinese Patients3Recruiting
NCT03072238Ipatasertib + Abiraterone + Prednisone for mCRPC3Completed
NCT03337724Ipatasertib + Paclitaxel for PIK3CA/AKT1/PTEN-Altered TNBC or HR+/HER2- Breast Cancer3Completed
NCT04177108Ipatasertib + Atezolizumab + Paclitaxel for TNBC3Completed
NCT04650581Fulvestrant + Ipatasertib for ER+ Breast Cancer After CDK4/6i Progression3Active
NCT02423603PAKT: AZD5363 (Capivasertib) + Paclitaxel in TNBC2Completed
NCT02451956AZD5363 + Paclitaxel in PIK3CA-mutant Gastric Adenocarcinoma (2nd-line)2Completed
NCT05008055Capivasertib in Relapsed/Refractory B-cell Non-Hodgkin Lymphoma2Completed
NCT05593497Neoadjuvant Intensified ADT + Abiraterone + Capivasertib for High-Risk Localized Prostate Cancer with PTEN Loss2Recruiting
NCT05720260Immunotherapy + Hormone Therapy + Capivasertib (AKT Inhibitor) for Premenopausal ER+ MBC2Recruiting
NCT04439123AZD5363 as Targeted Treatment in Cancers with AKT Genetic Changes (MATCH-Y)2Active
NCT02208375AZD2014 (mTORC1/2 inhibitor) or AZD5363 (AKT inhibitor) for Recurrent Endometrial/Ovarian Cancer1Active
NCT02338622Olaparib + AZD5363 (ComPAKT)1Completed
NCT03310541AZD5363 in Patients with AKT Mutations1Completed
NCT01226316AZD5363 Safety & Tolerability with Different Treatment Schedules1Completed

Note: 100+ trials identified; shown are highest-phase oncology trials. Additional trials span leishmaniasis (miltefosine), cardiovascular disease (rupitasertib), and other indications.

Pharmacogenomics & Drug-Gene Interactions

PharmGKB VIP Gene Status: AKT1 is designated a Very Important Pharmacogene (VIP) with documented variant annotations and clinical pharmacogenomic data.

Known drug-gene interactions affecting response:

  1. Gefitinib — 22 clinical annotations; 180 variant annotations; pathway association (EGFR signaling)
  2. Erlotinib — 5 clinical annotations; 52 variant annotations; pathway association (EGFR signaling)
  3. Capivasertib — Direct AKT1 inhibitor; no published variant-specific dosing yet
  4. Risperidone — 78 clinical annotations; 385 variant annotations
  5. Carboplatin — 65 clinical annotations; 134 variant annotations
  6. Afatinib — 9 variant annotations
  7. Methamphetamine — 17 clinical annotations; 47 variant annotations

Dosing guidelines: No formal AKT1-variant-specific dosing guidelines published in standard references. However, capivasertib and ipatasertib are approved for PIK3CA/AKT1/PTEN-altered cancers with standard dosing (capivasertib 400 mg BD day 1-4 in 7-day cycles; ipatasertib 400 mg daily). Response optimization relies on tumor genetic profiling (presence of activating AKT1 mutations, PTEN loss, or PIK3CA mutations) rather than germline AKT1 polymorphisms.

Expression profiles

Tissue Expression (Bgee)

AKT1 shows ubiquitous expression across tissues with high average expression score (87.37). All 30 top-expressed tissues show “present” calls with scores above 96, indicating consistent high expression across diverse tissue types.

RankTissue/Anatomical StructureExpression ScoreQuality
1Stromal cell of endometrium98.05Gold
2Ganglionic eminence97.94Gold
3Endometrium epithelium97.88Gold
4Left adrenal gland97.72Gold
5Left adrenal gland cortex97.72Gold
6Right adrenal gland97.54Gold
7Lower esophagus muscularis layer97.52Gold
8Lower esophagus97.50Gold
9Right adrenal gland cortex97.44Gold
10Ventricular zone97.42Gold
11Cortical plate97.28Gold
12Right coronary artery97.16Gold
13Gall bladder97.10Gold
14Esophagogastric junction muscularis propria97.06Gold
15Body of stomach97.02Gold
16Muscle layer of sigmoid colon97.00Gold
17Ascending aorta96.99Gold
18Thoracic aorta96.99Gold
19Popliteal artery96.92Gold
20Tibial artery96.91Gold
21Aorta96.88Gold
22Adrenal cortex96.78Gold
23Adrenal gland96.76Gold
24Descending thoracic aorta96.76Gold
25Upper lobe of left lung96.68Gold
26Apex of heart96.67Gold
27Right lung96.58Gold
28Granulocyte96.48Gold
29Left coronary artery96.48Gold
30Right lobe of thyroid gland96.25Gold

Key patterns:

  • Endocrine tissues: High expression in adrenal glands (97.5–97.7), thyroid (96.25)
  • Cardiovascular: Consistent high expression in all major arteries and cardiac regions (96.5–97.2)
  • Reproductive: Strong expression in endometrium and stromal tissues (98.05, 97.88)
  • Nervous system: High in brain developmental zones (ganglionic eminence 97.94, cortical plate 97.28, ventricular zone 97.42)
  • Gastrointestinal: Strong across esophagus, stomach, and colon smooth muscle layers (97.0–97.5)
  • Immune cells: Present in granulocytes (96.48)

Expression breadth: 273 present calls / 291 total conditions = 94% tissue coverage — consistent with a ubiquitous kinase involved in proliferation and survival signaling.

Cell-Type & Single-Cell Expression

Biobtree contains limited indexed cell-type specificity datasets for AKT1. Expression is broadly distributed across cell types given its role as a core survival kinase in the PI3K/AKT pathway. Known cell-type patterns from literature:

  • Immune cells: High in T cells, B cells, macrophages (PI3K/AKT critical for activation and survival)
  • Endothelial cells: Elevated (angiogenesis and vessel maintenance)
  • Epithelial cells: High across multiple tissue barriers
  • Stromal/fibroblasts: Highest expression in endometrial stroma (98.05 score)

Human Cell Atlas and Tabula Sapiens single-cell datasets show AKT1 as broadly expressed with some enrichment in immune lineages and endothelial compartments, reflecting its role in proliferation and anti-apoptotic signaling across cell types.

Disease associations

Mendelian / Monogenic Diseases

DiseaseOMIMMondoOrphanetInheritanceEvidence
Proteus syndrome176920MONDO:0008318ORPHA:744Autosomal dominantDefinitive
Cowden syndrome 6 (PTEN-related Cowden syndrome variant)615109MONDO:0014048ORPHA:201UnknownLimited
Cowden diseaseORPHA:201Autosomal dominantSupportive

Note: AKT1 mutations cause a minority of Cowden/Cowden-like syndrome cases; PTEN mutations are the predominant cause. Proteus syndrome is caused by somatic activating mutations in AKT1 (affecting ~10-20% of cases).

Phenotype Associations (Top 30+ HPO Terms)

HPO IDPhenotype
HP:0000006Autosomal dominant inheritance
HP:0001513Obesity
HP:0001528Hemihypertrophy
HP:0001548Overgrowth
HP:0002664Neoplasm
HP:0003002Breast carcinoma
HP:0003003Colon cancer
HP:0000256Macrocephaly
HP:0000238Hydrocephalus
HP:0001249Intellectual disability
HP:0001250Seizure
HP:0000518Cataract
HP:0000501Glaucoma
HP:0000620Blindness
HP:0002861Melanoma
HP:0002890Thyroid carcinoma
HP:0012114Endometrial carcinoma
HP:0005584Renal cell carcinoma
HP:0010788Testicular neoplasm
HP:0001004Lymphedema
HP:0001012Multiple lipomas
HP:0001028Hemangioma
HP:0004390Hamartomatous polyposis
HP:0007565Multiple cafe-au-lait spots
HP:0000982Palmoplantar keratoderma
HP:0009720Adenoma sebaceum
HP:0001067Neurofibroma
HP:0001156Brachydactyly
HP:0006101Finger syndactyly
HP:0001387Joint stiffness

Complex Disease / GWAS Associations

Trait/DiseaseEffectStudyP-value
Lipid Metabolism
HDL cholesterolAKT1GCST0066113 × 10⁻²⁶
Apolipoprotein A1 levelsAKT1GCST0102415 × 10⁻⁵⁷
HDL cholesterol levelsAKT1GCST0102424 × 10⁻³⁸
HDL cholesterolZBTB42-VESTARGCST0028998 × 10⁻⁹
Cancer
Endometrial cancerAKT1GCST0035244 × 10⁻⁸
Endometrial endometrioid carcinomaAKT1GCST0035254 × 10⁻⁸
Metabolic/Immune
Vitamin C levelsAKT1GCST0118161 × 10⁻⁸
Neutrophil countAKT1GCST900023987 × 10⁻⁹

Summary: AKT1 variants are strongly associated with HDL cholesterol and lipid metabolism (most significant associations); secondary associations with cancer risk (endometrial) and immune parameters. Rare variants cause monogenic Proteus syndrome and contribute to Cowden-like syndrome.

Structured Data Sources

Generated with Claude Haiku 4.5 + BioBTree MCP, drawing on data BioBTree aggregates from 45 biological databases. Every identifier and figure traces to a reproducible API call (listed below).

Further analyze this answer or run your own queries with BioBTree MCP.

Datasets: alphafold, alphamissense, antibody, bgee, biogrid_interaction, ccds, chembl_molecule, chembl_target, clinical_trials, clinvar, collectri, ensembl, entrez, esm2_similarity, exon, gencc, go, gtopdb, gtopdb_interaction, gtopdb_ligand, gwas, hgnc, hpa, hpo, intact, interpro, mim, mondo, msigdb, orphanet, ortholog, paralog, pdb, pfam, pharmgkb, pharmgkb_gene, reactome, refseq, scxa, smart, spliceai, string, string_interaction, transcript, uniprot
Generated: 2026-05-26 — For the latest data, query BioBTree directly via MCP or API.
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