TP53 Transcription Factor Network & Regulatory Target Analysis

TP53 is the master tumor suppressor regulating cell fate decisions. For a comprehensive transcription factor network analysis: 1. How many genes does …

TP53 is the master tumor suppressor regulating cell fate decisions. For a comprehensive transcription factor network analysis: 1. How many genes does TP53 regulate? List key targets with regulation type. 2. Which TP53 target genes encode cell surface receptors? 3. How many JASPAR binding profiles exist? What is the binding motif family? 4. How many evolutionarily similar proteins exist? 5. How many proteins share sequence homology? 6. How many high-confidence protein interactions? 7. What transcription factors regulate TP53 itself?

Structured Data Sources

This answer was generated using Claude Opus 4.5 + BioBTree MCP, querying 8 biological databases.

Further analyze this answer or run your own queries with BioBTree MCP.

Datasets: cellphonedb, collectri, diamond_similarity, esm2_similarity, hgnc, jaspar, string_interaction, uniprot
Generated: 2026-03-26 — For the latest data, query BioBTree directly via MCP or API.
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TP53

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TP53 Comprehensive Transcription Factor Network Analysis

1. Target Genes Regulated by TP53 Total targets: ~343 genes (based on CollecTRI database via HGNC:11998) Key Target Genes with Regulation Type:

TargetFunctionRegulationConfidence
CDKN1A (p21)Cell cycle arrestActivationHigh (382 citations)
BAXPro-apoptoticActivationHigh (71 citations)
BBC3 (PUMA)Pro-apoptoticActivationHigh (36 citations)
CDKN2A (p16)Cell cycleActivationHigh (46 citations)
GADD45ADNA repair/stressActivationHigh (45 citations)
BCL2Anti-apoptoticRepressionHigh (45 citations)
FASDeath receptorActivationHigh (21 citations)
MDM2p53 negative regulatorActivationHigh
CCND1Cyclin D1RepressionHigh (22 citations)
BIRC5 (Survivin)Anti-apoptoticRepressionHigh (30 citations)
BRCA1/BRCA2DNA repairActivationHigh
EGFRGrowth receptorActivationHigh (19 citations)
IGF1RInsulin-like growthRepressionHigh
NOTCH1Developmental signalingRepressionHigh

2. Cell Surface Receptor Targets TP53 regulates ~20+ cell surface receptor genes:

ReceptorTypeRegulationFunction
FAS (CD95)Death receptorActivationApoptosis signaling
TNFRSF10A (DR4)Death receptorActivationTRAIL-induced apoptosis
TNFRSF10B (DR5)Death receptorActivationTRAIL-induced apoptosis
EGFRRTKActivationGrowth factor signaling
ERBB2 (HER2)RTK-Growth factor signaling
IGF1RRTKRepressionInsulin/IGF signaling
INSRRTKRepressionInsulin signaling
FLT1 (VEGFR1)RTKActivationAngiogenesis
FGFR1RTK-FGF signaling
EPHA2RTKActivationEphrin signaling
EPHB4RTK-Ephrin signaling
DDR1RTK-Collagen receptor
DCCNetrin receptor-Axon guidance
ADGRB1/2 (BAI1/2)Adhesion GPCR-Anti-angiogenic
CXCR4Chemokine GPCR-Cell migration
NOTCH1DevelopmentalRepressionCell fate decisions
CD40TNFRSF-Immune signaling
CD44Hyaluronan receptor-Cell adhesion
GHRCytokine receptor-Growth hormone
GHRHRGPCR-Growth hormone releasing

3. JASPAR Binding Profiles 3 binding profiles exist:

Profile IDVersionCollectionMethod
MA0106.11CORE-
MA0106.22CORE-
MA0106.33COREHT-SELEX
Binding Motif Family:
  • Class: p53 domain factors
  • Family: p53-related factors
  • Taxonomic group: Vertebrates
  • Species: Homo sapiens

4. Evolutionarily Similar Proteins (ESM2 Structural Similarity) 57 proteins share evolutionary/structural similarity:

ProteinSimilarity ScoreOrganism
P04637 (self)0.9998Human
O57538, Q921431.0000Various
P134811.0000Vertebrate orthologs
P56423, P56424, P612601.0000Primate p53
P67938, P679391.0000p53 orthologs
Q15306, Q642870.9997p63/p73 family
O08674 (mouse)0.9994Mouse
P79734 (zebrafish)0.9994Zebrafish
Average similarity score: ~0.99

5. Sequence Homology Proteins (DIAMOND) 40 proteins share sequence homology:

Protein% IdentityBit ScoreNotes
P56423, P56424, P61260100%773Identical sequences
P67938, P67939100%748p53 variants
O88898, Q9JJP699.6%1330-1333High similarity
O57538, Q9214399.4%641Vertebrate orthologs
O15350, Q9XSK897.5%1242-1243p63 family
Q9H3D496.3%1301p73 family
O0918591.2%707Mouse p53
P0234086.2%669Rodent p53
P7973477.8%567Zebrafish p53

6. High-Confidence Protein Interactions (STRING score ≥900) 114 high-confidence protein interactions: Top Interactors (score 999):

ProteinNameInteraction Type
Q00987MDM2Primary negative regulator
P10415BCL2Apoptosis pathway
P38398BRCA1DNA repair complex
Key Functional Partners (score 990-998):
ProteinNameFunction
Q12888TP53BP1DNA damage response
Q07817BCL2L1 (BCL-XL)Anti-apoptotic
Q09472EP300Transcriptional co-activator
O15151MDM4p53 negative regulator
P01106MYCOncogenic TF
Q92793CREBBP (CBP)Histone acetyltransferase
P07900HSP90AA1Chaperone
Q06609RAD51Homologous recombination
P38936CDKN1A (p21)Cell cycle inhibitor
P42771CDKN2A (p16)Cell cycle inhibitor
P00533EGFRGrowth receptor

7. Transcription Factors Regulating TP53 ~100+ transcription factors regulate TP53 expression: Major Regulators:

TFxref countFunction
MYC20Oncogenic, represses TP53
NFKB19Inflammatory response
HIF1A16Hypoxia response
RELA15NF-kB subunit
SP115Basal transcription
JUN13AP-1 component
ESR113Estrogen receptor
AP112Stress response
PAX112Development
E2F111Cell cycle
MYCN11Oncogenic
NKX3-111Tumor suppressor
NFKB111Inflammatory
ATF311Stress response
EGR111Immediate early gene
DNMT111DNA methylation
CTCF10Chromatin organization
MDM410p53 pathway
ETS1/28-10Developmental
STAT1/36-9Cytokine signaling
FOXO1/37Metabolic regulation
KLF49Pluripotency factor
CEBPB9Metabolic stress
SIRT1/67Histone deacetylases

Summary Statistics

MetricCount
Target genes regulated~343
Cell surface receptor targets~20+
JASPAR binding profiles3
Evolutionarily similar proteins57
Sequence homology proteins40
High-confidence interactions (≥900)114
TFs regulating TP53~100+
Total STRING interactions14,764
Total cross-references59,810
TP53 sits at the center of a massive regulatory network controlling cell fate decisions through transcriptional regulation of cell cycle, apoptosis, DNA repair, and metabolic genes, while being itself regulated by numerous stress-responsive and oncogenic transcription factors.