# NM_021120.4(DLG3):c.463C>T (p.Pro155Ser)

https://sugi.bio/variant/dlg3-p-pro155ser

## Summary

**DLG3 p.Pro155Ser (c.463C>T)** is classified **Uncertain significance** in DLG3 (ClinVar, Single submitter, 1 submitter; last evaluated 2025-01-31). AlphaMissense: likely benign (0.2571); Very rare (popmax 0.00296%, eas); Associated with intellectual disability, X-linked 90.

**At a glance:** Uncertain significance · review: Single submitter · 1 submitter · 1 linked condition · AlphaMissense likely benign · very rare (popmax 0.00296%, eas)

## For patients & families {#patients}

This is a change in the DLG3 gene that is currently of uncertain significance — a definitive interpretation has not yet been established, submitted by 1 submitter, and is linked to intellectual disability, X-linked 90.

*Reference information, not medical advice, and not a prediction for any individual. A genetic counselor or clinician can interpret what it means for you and your family.*

**Support & next steps:** [Condition overview & support (NIH GARD)](https://rarediseases.info.nih.gov/diseases/0022690/index) · [Find a genetic counselor (NSGC)](https://findageneticcounselor.nsgc.org/).

## Identity {#identity}

| Field | Value |
| --- | --- |
| Gene | [DLG3](https://sugi.bio/atlas/gene/DLG3/) |
| Protein change (HGVS p.) | p.Pro155Ser |
| Coding change (HGVS c.) | c.463C>T |
| MANE Select transcript | [NM_021120](https://www.ncbi.nlm.nih.gov/nuccore/NM_021120) |
| Variant type | single nucleotide variant |
| Location | chrX:70449413-70449413 (GRCh38) |
| ClinVar | [VCV3770145](https://www.ncbi.nlm.nih.gov/clinvar/variation/3770145/) |

**All HGVS expressions:** `NC_000023.10:g.69669263C>T`, `NC_000023.11:g.70449413C>T`, `NG_015849.2:g.9559C>T`, `NM_021120.4:c.463C>T`

## Computational & population evidence {#evidence}

**Concordance:** ClinVar classifies this **uncertain**; the computational predictors unanimously lean **tolerated** — a triage signal, not a reclassification.

- AlphaMissense **likely benign** (0.2571)
- REVEL 0.207 (benign-leaning) — agrees with AlphaMissense
- SaProt LLR -8.989 (tolerated) — agrees with AlphaMissense
- → **In-silico consensus:** AlphaMissense, REVEL and SaProt all call this tolerated
- Conservation: phyloP 3.251, GERP -1.76, phastCons 1 — highly conserved (differs from AlphaMissense)
- CADD: **PHRED 24.3**. No single ClinGen-calibrated threshold exists and the expert panels that use CADD disagree — GUCY2D/AIPL1 require ≥ 20.0 for an untranslated-region variant, ABCA4 ≥ 28.1 for a synonymous or indel variant, the Pulmonary Hypertension panel imports Pejaver 2022's missense-calibrated numbers, and the Antibody Deficiencies panel declines CADD entirely for PIK3CD. CADD v1.7 also incorporates Zoonomia phyloP, so it partly re-expresses the conservation above. *Descriptive; no threshold applied.*
- Regional missense constraint: **o/e 0.46** across the covering region of the MANE Select transcript (70,448,946–70,450,744), spanning 1,798 bp. Chi-square p 1.1e-11. Describes the region, not this allele; gnomAD v4.1.1's own constrained determination. *Descriptive; no ACMG code applied.*
- SpliceAI: **not assessed** — no allele at this position is in our SpliceAI release, either because it lies outside the scored MANE transcript scope or because every allele is below Δ0.2. We cannot tell which, so this claims neither; absence is not evidence against a splicing effect.
- Population frequency (gnomAD v4.1): grpmax **0.00296%** (eas) · 1/33,830 in the highest group · 3/1,097,982 alleles overall — very rare (popmax 0.00296%, eas).

| Ancestry group | Allele frequency | 1 in |
| --- | --- | --- |
| Remaining individuals (not grpmax-eligible) | 0.0042% | 23,818 |
| East Asian (grpmax) | 0.00296% | 33,830 |

Remaining individuals shows a higher raw frequency than the grpmax group. gnomAD excludes bottlenecked groups — Finnish, Ashkenazi Jewish, Amish and "Remaining individuals" — from grpmax, because founder effects make their frequencies unrepresentative for filtering. The table is sorted by frequency, so an excluded group can sit above the grpmax row.

*Computational predictors are not independent (ClinGen Variant Classification guidance): AlphaMissense (Cheng et al. 2023) carries the ACMG weight for missense and conservation (phyloP/GERP/phastCons) for non-missense; REVEL (Pejaver-2022 calibrated strength bands) and SaProt (Su et al. 2023, structure-aware, ClinVar-independent protein language model, raw LLR) are shown as orthogonal agreement signals, not additive. For frequency, the gnomAD v4.1 grpmax filtering allele frequency (FAF95) is ClinGen's designated BA1/BS1 input (gnomAD v4 guidance v3.0, June 2025); BA1/BS1 thresholds are disease-specific, not a blanket 5%. Predictions, not a clinical determination.*

## Gene constraint & dosage {#gene-context}

**DLG3** population constraint: ClinGen dosage — haploinsufficiency 3, triplosensitivity 0 (0–3 scale). *Lower LOEUF and higher pLI indicate a gene that tolerates loss-of-function poorly; ClinGen dosage is the curated haploinsufficiency call.*

Curated gene–disease validity (ClinGen):

- **non-syndromic X-linked intellectual disability** — Definitive (XL)

## Protein context {#protein}

Residue **155** lies in the pdz 1 domain.

AlphaFold model confidence (whole protein): pLDDT 74.35, 49% of residues very-high.

**3 experimental structure(s)** (PDB): [1UM7](https://www.rcsb.org/structure/1UM7), [2FE5](https://www.rcsb.org/structure/2FE5), [2I1N](https://www.rcsb.org/structure/2I1N).

## What this gene does {#gene-function}

*Describes the biological roles of DLG3 (the gene this variant disrupts) — not effects measured for this specific variant. A damaging variant is expected to affect these functions and pathways; the degree depends on the variant.*

**How this variant is thought to act:** DLG3's established molecular role includes kinase binding. p.Pro155Ser falls in the pdz 1 domain.


### Pathways affected {#pathways}

DLG3 participates in **10 Reactome pathways** (⚕ = disease pathway):

| Pathway (Reactome) | Evidence |
| --- | --- |
| [Activation of Ca-permeable Kainate Receptor](https://reactome.org/content/detail/R-HSA-451308) | curated |
| [RAF/MAP kinase cascade](https://reactome.org/content/detail/R-HSA-5673001) | curated |
| [Synaptic adhesion-like molecules](https://reactome.org/content/detail/R-HSA-8849932) | curated |
| [Assembly and cell surface presentation of NMDA receptors](https://reactome.org/content/detail/R-HSA-9609736) | electronic |
| [Long-term potentiation](https://reactome.org/content/detail/R-HSA-9620244) | electronic |
| [Negative regulation of NMDA receptor-mediated neuronal transmission](https://reactome.org/content/detail/R-HSA-9617324) | electronic |
| [Neurexins and neuroligins](https://reactome.org/content/detail/R-HSA-6794361) | electronic |
| [NrCAM interactions](https://reactome.org/content/detail/R-HSA-447038) | electronic |
| [Ras activation upon Ca2+ influx through NMDA receptor](https://reactome.org/content/detail/R-HSA-442982) | electronic |
| [Unblocking of NMDA receptors, glutamate binding and activation](https://reactome.org/content/detail/R-HSA-438066) | electronic |

### Molecular function & processes (GO — experimentally supported) {#go}

**Molecular function:** kinase binding, phosphatase binding.

## Clinical significance {#significance}

**Uncertain significance** — review status Single submitter *(criteria provided, single submitter)*, last evaluated 2025-01-31.

**Submitter consensus:** unanimous (1/1 Uncertain significance).

### Submitter classifications {#submitters}

| Submitter | Classification | Review | Method | Date |
| --- | --- | --- | --- | --- |
| Equipe Genetique des Anomalies du Developpement, Université de Bourgogne | Uncertain significance | criteria provided, single submitter | curation | 2025-01-31 |

## Associated conditions {#conditions}

[intellectual disability, X-linked 90](https://sugi.bio/atlas/disease/intellectual-disability-x-linked-90/).

**Reported phenotype names (ClinVar):** Intellectual disability, X-linked 90, INTELLECTUAL DEVELOPMENTAL DISORDER, X-LINKED 90, DLG3-Related X-Linked Nonsyndromic Mental Retardation.

## Similar variants {#similar}

Related DLG3 variant pages (same residue, condition, or type): [DLG3 p.Arg217Ter](/variant/dlg3-p-arg217ter), [DLG3 p.Arg756Ter](/variant/dlg3-p-arg756ter), [DLG3 p.Arg217Gln](/variant/dlg3-p-arg217gln), [DLG3 p.Arg472His](/variant/dlg3-p-arg472his), [DLG3 p.Arg661Trp](/variant/dlg3-p-arg661trp), [DLG3 p.Gly83Glu](/variant/dlg3-p-gly83glu), [DLG3 p.His2Pro](/variant/dlg3-p-his2pro), [DLG3 p.Ile748Thr](/variant/dlg3-p-ile748thr).

### Data sources & attribution {#sources}

ClinVar (NCBI — public domain, built 2026-10-11); gnomAD v4.1 (Broad Institute, built 2026-10-06); AlphaMissense (Cheng et al. 2023, © Google DeepMind — CC BY 4.0, built 2026-01-27); REVEL (Ioannidis et al. 2016 — ODbL, built 2026-07-13); SaProt (Su et al. 2023 — MIT, built 2026-07-14); SpliceAI (Illumina — free for non-commercial use, built 2026-10-13); CADD (Rentzsch et al. 2019, University of Washington — free for non-commercial use, built 2026-10-10); Conservation (phyloP/phastCons/GERP) (UCSC hg38 470-way mammal (Hiller Lab) + Ensembl Compara, built 2026-07-12); gnomAD Non-coding Constraint (Gnocchi) (Chen et al. 2024, Broad Institute — CC0, built 2026-10-11); gnomAD Regional Missense Constraint (Broad Institute — CC0, built 2026-10-13); Pangolin (Zeng & Li 2022 — CC BY 4.0, built 2026-10-11); Repeat regions (UCSC — RepeatMasker, TRF, segmental duplications, built 2026-10-11); ClinGen (ClinGen, built 2026-09-22); UniProt (CC BY 4.0); Reactome / GO (CC BY 4.0, built 2026-09-23); MaveDB (per-record license, built 2026-10-11); CIViC (CC0, built 2026-09-22); Orphanet (Orphanet, built 2026-06-12)

*Primary record: NCBI ClinVar (variation 3770145). Classifications reflect these databases as of the page's build date and may change. Free reference use — not medical advice; consult the primary submitters and a clinician.*