Is ARAP1 p.Arg166Ser (c.496C>A) pathogenic?

ARAP1 p.Arg166Ser (c.496C>A) is classified Uncertain significance in ARAP1 (ClinVar, Single submitter, 1 submitter; last evaluated 2024-07-17). AlphaMissense: likely benign (0.1194); Very rare (popmax 0.00987%, amr).

How common is ARAP1 p.Arg166Ser (c.496C>A) in the general population?

ARAP1 p.Arg166Ser (c.496C>A) is very rare (popmax 0.00987%, amr) (gnomAD).

What do computational predictors say about ARAP1 p.Arg166Ser (c.496C>A)?

AlphaMissense predicts likely benign (score 0.1194) — an in-silico prediction, not a clinical call.

ARAP1 p.Arg166Ser

c.496C>A · VCV3419377

Uncertain significance Single submitter

Classification

Uncertain significance

ClinVar

Review

Single submitter ★☆☆☆

ClinVar · 1 submitter

AlphaMissense

likely benign

0.1194 · ACMG-weighted

gnomAD v4.1

Rare

very rare (popmax 0.00987%, amr)

Missense predictors

0/3 damaging

AlphaMissense, REVEL, SaProt

Computational evidence

Protein change
AlphaMissense	0.1194	likely benign
REVEL	0.028	moderately benign-leaning
SaProt	LLR -5.72	tolerated
⇒ AlphaMissense, REVEL and SaProt all call this tolerated.
Splicing
SpliceAI	—	not assessed · no allele here is in the release
Constraint at this position
Conservation	phyloP 3.2	GERP 0.4, phastCons 0.98
Regional constraint	o/e 1.20	unconstrained · MANE · 14 kb · p 0.023 · regional, not allelic
Overall
CADD	15.58	PHRED · no single calibrated threshold — expert panels disagree

ClinVar classifies this uncertain; the computational predictors unanimously lean tolerated — a triage signal, not a reclassification

AlphaMissense carries the ACMG weight for missense; REVEL and SaProt are independent agreement signals.

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 0.00987% Admixed American · 5/50,676 alleles

Filtering AF 0.00384% ClinGen BA1/BS1 input FAF95, joint · FAF99 0.00249%

Overall 0.000661% 10/1,513,168 alleles · very rare

Ancestry group	Frequency	1 in
Admixed Americangrpmax	0.00987%	10,135
East Asian	0.00239%	41,840
Finnishnot grpmax-eligible	0.00198%	50,386
European (non-Finnish)	0.000266%	376,442

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: ARAP1
Protein: p.Arg166Ser
Coding: c.496C>A
MANE Select: NM_001040118
Type: single nucleotide variant
Location: chr11:72726633 (GRCh38)
Gene constraint: LOEUF 0.572 · pLI <0.01 (gnomAD)
Structure: AlphaFold pLDDT 75.81 · 1 PDB

Full ARAP1 gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Similar variants

ARAP1 p.Arg166Gly Uncertain significance ★ ⚠ same residue
ARAP1 p.Gln1047Glu Benign ★★ same type
ARAP1 p.Pro1039= Benign ★★ same type
ARAP1 p.Pro254Leu Benign ★★ same type
ARAP1 p.Val1408Met Benign ★★ ⚠ same type
ARAP1 p.Ala1046Ser Uncertain significance ★ ⚠ same type
ARAP1 p.Ala1124Thr Uncertain significance ★ ⚠ same type
ARAP1 p.Ala1199Thr Uncertain significance ★ ⚠ same type

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/arap1-p-arg166ser (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown