Is BRCA2 p.Pro527Ser (c.1579C>T) pathogenic?

BRCA2 p.Pro527Ser (c.1579C>T) is classified Conflicting classifications of pathogenicity in BRCA2 (ClinVar, Conflicting submitters, 5 submitters; last evaluated 2025-10-05). AlphaMissense: likely benign (0.078); Very rare (popmax 0.00669%, sas); Associated with hereditary neoplastic syndrome.

What condition is associated with BRCA2 p.Pro527Ser (c.1579C>T)?

BRCA2 p.Pro527Ser (c.1579C>T) is associated with hereditary neoplastic syndrome, hereditary breast ovarian cancer syndrome (ClinVar).

How common is BRCA2 p.Pro527Ser (c.1579C>T) in the general population?

BRCA2 p.Pro527Ser (c.1579C>T) is very rare (popmax 0.00669%, sas) (gnomAD).

What do computational predictors say about BRCA2 p.Pro527Ser (c.1579C>T)?

AlphaMissense predicts likely benign (score 0.078) — an in-silico prediction, not a clinical call.

BRCA2 p.Pro527Ser

c.1579C>T · rs758025325 · VCV819591

Conflicting classifications of pathogenicity Conflicting submitters

Classification

Conflicting classifications of pathogenicity

ClinVar

Review

Conflicting submitters ★☆☆☆

ClinVar · 5 submitters

AlphaMissense

likely benign

0.078 · ACMG-weighted

gnomAD v4.1

Rare

very rare (popmax 0.00669%, sas)

Conditions

hereditary neoplastic syndrome

Computational evidence

Protein change
AlphaMissense	0.078	likely benign
REVEL	0.291	indeterminate
SaProt	—	not scored · why
Splicing
SpliceAI	<0.2	no splice effect predicted · four deltas, not one · how we know
Constraint at this position
Conservation	phyloP -2.9	GERP -4.6
Regional constraint	o/e 1.03	unconstrained · MANE · 47 kb · p 0.059 · regional, not allelic
Overall
CADD	0.327	PHRED · no single calibrated threshold — expert panels disagree

AlphaMissense carries the ACMG weight for missense; REVEL is an independent agreement signal.

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 0.00669% South Asian · 6/89,706 alleles

Filtering AF 0.0029% ClinGen BA1/BS1 input FAF95, joint · FAF99 0.00197%

Overall 0.000497% 8/1,609,698 alleles · very rare exomes 8/1,457,488 · genomes 0/152,210 · joint denominator

Ancestry group	Frequency	1 in
South Asiangrpmax	0.00669%	14,951
Remaining individualsnot grpmax-eligible	0.00161%	62,280
African / African-American	0.00134%	74,584

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: BRCA2
Protein: p.Pro527Ser
Coding: c.1579C>T
MANE Select: NM_000059
dbSNP: rs758025325
Type: single nucleotide variant
Location: chr13:32333057 (GRCh38)
Gene constraint: LOEUF 0.803 · pLI <0.01 · ClinGen HI 3/3 (gnomAD)

Full BRCA2 gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Disease context

hereditary neoplastic syndrome — disease report on Sugi Atlas → Associated genes, phenotypes, clinical trials & treatments

Also linked: hereditary breast ovarian cancer syndrome

Similar variants

BRCA2 p.Pro527Arg Uncertain significance ★★ ⚠ same residue
BRCA2 p.Pro527Ala Conflicting classifications of pathogenicity ★ ⚠ same residue
BRCA2 p.Pro527= Likely benign ★ same residue
BRCA2 c.316+1G>T Pathogenic ★★★ same condition
BRCA2 c.316+2T>C Pathogenic ★★★ same condition
BRCA2 c.316+5G>A Pathogenic ★★★ same condition
BRCA2 c.316+5G>C Pathogenic ★★★ same condition
BRCA2 c.475+1G>A Pathogenic ★★★ same condition

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/brca2-p-pro527ser (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown