MARS2 — 277 ClinVar variants
All ClinVar germline MARS2 variants across every classification, each assembled from ClinVar, gnomAD, AlphaMissense, conservation and more. 163 flagged for review →
2 Pathogenic · 3 Likely pathogenic · 11 Conflicting · 6 Benign · 8 Benign/Likely benign · 55 Likely benign · 192 Uncertain significance
Full MARS2 gene report on Sugi Atlas → Gene function, pathways, protein structure, constraint & interactions⚑ Evidence disagreements 163
Variants where the independent computational evidence doesn't line up with the clinical picture — a QC signal for a second look, not a reclassification.
Predictors agree, ClinVar conflicts 5
ClinVar submitters conflict, but the independent predictors unanimously agree — a QC review flag, not a reclassification.
Predictors lean, ClinVar uncertain 133
ClinVar classifies these uncertain (VUS), but the independent predictors unanimously lean one way — a triage signal for review, not a reclassification.
- MARS2 p.Ala141Val Uncertain significance Multiple submitters
- MARS2 p.Ala402Ser Uncertain significance Multiple submitters
- MARS2 p.Arg224Gln Uncertain significance Multiple submitters
- MARS2 p.Arg409Gly Uncertain significance Multiple submitters
-
MARS2 p.Arg414Ile
Uncertain significance
Multiple submitters
spastic ataxia 3
- MARS2 p.Arg568Gly Uncertain significance Multiple submitters
- MARS2 p.Arg592Gln Uncertain significance Multiple submitters
- MARS2 p.Arg79Gln Uncertain significance Multiple submitters
- MARS2 p.Cys175Ser Uncertain significance Multiple submitters
- MARS2 p.Glu249Lys Uncertain significance Multiple submitters
- MARS2 p.Gly574Val Uncertain significance Multiple submitters
- MARS2 p.His338Arg Uncertain significance Multiple submitters
- MARS2 p.Ile266Thr Uncertain significance Multiple submitters
- MARS2 p.Leu159Val Uncertain significance Multiple submitters
- MARS2 p.Leu243Phe Uncertain significance Multiple submitters
- MARS2 p.Leu2Arg Uncertain significance Multiple submitters
- MARS2 p.Pro230Arg Uncertain significance Multiple submitters
- MARS2 p.Pro230Leu Uncertain significance Multiple submitters
- MARS2 p.Pro321Leu Uncertain significance Multiple submitters
- MARS2 p.Ser30Cys Uncertain significance Multiple submitters
- MARS2 p.Thr13Met Uncertain significance Multiple submitters
- MARS2 p.Thr183Ile Uncertain significance Multiple submitters
- MARS2 p.Trp151Leu Uncertain significance Multiple submitters
- MARS2 p.Trp151Ser Uncertain significance Multiple submitters
-
MARS2 p.Tyr165Cys
Uncertain significance
Multiple submitters
combined oxidative phosphorylation defect type 25
- MARS2 p.Tyr29Phe Uncertain significance Multiple submitters
- MARS2 p.Tyr558Cys Uncertain significance Multiple submitters
- MARS2 p.Val255Met Uncertain significance Multiple submitters
- MARS2 p.Ala128Ser Uncertain significance Single submitter
- MARS2 p.Ala146Gly Uncertain significance Single submitter
- MARS2 p.Ala180Val Uncertain significance Single submitter
- MARS2 p.Ala282Val Uncertain significance Single submitter
- MARS2 p.Ala295Thr Uncertain significance Single submitter
- MARS2 p.Ala36Ser Uncertain significance Single submitter
- MARS2 p.Ala423Gly Uncertain significance Single submitter
- MARS2 p.Ala503Ser Uncertain significance Single submitter
-
MARS2 p.Ala532Gly
Uncertain significance
Single submitter
spastic ataxia 3
- MARS2 p.Ala590Thr Uncertain significance Single submitter
- MARS2 p.Arg144Leu Uncertain significance Single submitter
- MARS2 p.Arg227Gln Uncertain significance Single submitter
- MARS2 p.Arg257Pro Uncertain significance Single submitter
- MARS2 p.Arg334His Uncertain significance Single submitter
- MARS2 p.Arg364Cys Uncertain significance Single submitter
- MARS2 p.Arg364His Uncertain significance Single submitter
- MARS2 p.Arg364Leu Uncertain significance Single submitter
- MARS2 p.Arg371Cys Uncertain significance Single submitter
- MARS2 p.Arg409Gln Uncertain significance Single submitter
- MARS2 p.Arg584Gly Uncertain significance Single submitter
- MARS2 p.Arg592Gly Uncertain significance Single submitter
- MARS2 p.Arg77Cys Uncertain significance Single submitter
- MARS2 p.Arg8Leu Uncertain significance Single submitter
- MARS2 p.Arg8Ser Uncertain significance Single submitter
- MARS2 p.Asn229Lys Uncertain significance Single submitter
- MARS2 p.Asn229Thr Uncertain significance Single submitter
- MARS2 p.Asn285Ile Uncertain significance Single submitter
- MARS2 p.Asn416Thr Uncertain significance Single submitter
- MARS2 p.Asp247Gly Uncertain significance Single submitter
- MARS2 p.Asp272Gly Uncertain significance Single submitter
- MARS2 p.Asp385His Uncertain significance Single submitter
- MARS2 p.Asp388Glu Uncertain significance Single submitter
- MARS2 p.Asp39Asn Uncertain significance Single submitter
- MARS2 p.Asp462Glu Uncertain significance Single submitter
- MARS2 p.Asp465Gly Uncertain significance Single submitter
- MARS2 p.Cys344Gly Uncertain significance Single submitter
- MARS2 p.Cys361Ser Uncertain significance Single submitter
- MARS2 p.Cys479Tyr Uncertain significance Single submitter
- MARS2 p.Cys562Phe Uncertain significance Single submitter
- MARS2 p.Cys73Phe Uncertain significance Single submitter
- MARS2 p.Gln123Leu Uncertain significance Single submitter
- MARS2 p.Gln482His Uncertain significance Single submitter
- MARS2 p.Glu296Asp Uncertain significance Single submitter
- MARS2 p.Glu398Gln Uncertain significance Single submitter
- MARS2 p.Glu432Gly Uncertain significance Single submitter
- MARS2 p.Glu550Lys Uncertain significance Single submitter
- MARS2 p.Gly11Glu Uncertain significance Single submitter
- MARS2 p.Gly167Ser Uncertain significance Single submitter
- MARS2 p.Gly270Glu Uncertain significance Single submitter
- MARS2 p.Gly291Asp Uncertain significance Single submitter
- MARS2 p.His239Leu Uncertain significance Single submitter
- MARS2 p.His316Leu Uncertain significance Single submitter
- MARS2 p.His316Tyr Uncertain significance Single submitter
- MARS2 p.His463Tyr Uncertain significance Single submitter
- MARS2 p.His560Tyr Uncertain significance Single submitter
- MARS2 p.Ile130Thr Uncertain significance Single submitter
- MARS2 p.Ile136Asn Uncertain significance Single submitter
- MARS2 p.Ile136Ser Uncertain significance Single submitter
- MARS2 p.Ile415Leu Uncertain significance Single submitter
- MARS2 p.Ile51Val Uncertain significance Single submitter
-
MARS2 p.Leu197His
Uncertain significance
Single submitter
spastic ataxia 3
- MARS2 p.Leu20Pro Uncertain significance Single submitter
- MARS2 p.Leu283Val Uncertain significance Single submitter
- MARS2 p.Leu524Pro Uncertain significance Single submitter
- MARS2 p.Leu7Phe Uncertain significance Single submitter
- MARS2 p.Leu9Gln Uncertain significance Single submitter
-
MARS2 p.Lys310Glu
Uncertain significance
Single submitter
combined oxidative phosphorylation defect type 25
- MARS2 p.Lys310Met Uncertain significance Single submitter
- MARS2 p.Lys347Arg Uncertain significance Single submitter
- MARS2 p.Lys347Glu Uncertain significance Single submitter
- MARS2 p.Lys458Asn Uncertain significance Single submitter
- MARS2 p.Met329Thr Uncertain significance Single submitter
-
MARS2 p.Pro267Ser
Uncertain significance
Single submitter
spastic ataxia 3
- MARS2 p.Pro302Ala Uncertain significance Single submitter
- MARS2 p.Pro302Ser Uncertain significance Single submitter
- MARS2 p.Pro358Arg Uncertain significance Single submitter
- MARS2 p.Pro380Ser Uncertain significance Single submitter
- MARS2 p.Pro529Leu Uncertain significance Single submitter
- MARS2 p.Pro571Ser Uncertain significance Single submitter
- MARS2 p.Pro81Thr Uncertain significance Single submitter
- MARS2 p.Ser260Gly Uncertain significance Single submitter
- MARS2 p.Ser30Thr Uncertain significance Single submitter
- MARS2 p.Ser349Cys Uncertain significance Single submitter
- MARS2 p.Ser351Arg Uncertain significance Single submitter
- MARS2 p.Ser431Arg Uncertain significance Single submitter
- MARS2 p.Ser478Thr Uncertain significance Single submitter
- MARS2 p.Ser542Cys Uncertain significance Single submitter
- MARS2 p.Thr234Asn Uncertain significance Single submitter
- MARS2 p.Thr288Ser Uncertain significance Single submitter
- MARS2 p.Thr360Ala Uncertain significance Single submitter
- MARS2 p.Thr528Ile Uncertain significance Single submitter
- MARS2 p.Thr585Ser Uncertain significance Single submitter
- MARS2 p.Thr593Ala Uncertain significance Single submitter
- MARS2 p.Thr593Ile Uncertain significance Single submitter
- MARS2 p.Trp151Cys Uncertain significance Single submitter
- MARS2 p.Tyr28His Uncertain significance Single submitter
- MARS2 p.Tyr387Asn Uncertain significance Single submitter
- MARS2 p.Tyr387Cys Uncertain significance Single submitter
- MARS2 p.Tyr447Cys Uncertain significance Single submitter
- MARS2 p.Val195Ala Uncertain significance Single submitter
- MARS2 p.Val453Leu Uncertain significance Single submitter
- MARS2 p.Val453Met Uncertain significance Single submitter
- MARS2 p.Val487Ile Uncertain significance Single submitter
- MARS2 p.Val512Met Uncertain significance Single submitter
- MARS2 p.Ala461Gly Uncertain significance No assertion criteria
Predictors split 25
The independent predictors disagree with each other — read with care.
-
MARS2 p.Arg142Trp
Uncertain significance
Multiple submitters
combined oxidative phosphorylation defect type 25
- MARS2 p.Asn285Ser Uncertain significance Multiple submitters
- MARS2 p.Ile136Met Uncertain significance Multiple submitters
- MARS2 p.Ile276Leu Uncertain significance Multiple submitters
- MARS2 p.Ser156Phe Uncertain significance Multiple submitters
- MARS2 p.Ser418Phe Uncertain significance Multiple submitters
- MARS2 p.Tyr365Cys Uncertain significance Multiple submitters
- MARS2 p.Ala282Ser Uncertain significance Single submitter
- MARS2 p.Arg142Gln Uncertain significance Single submitter
- MARS2 p.Cys384Tyr Uncertain significance Single submitter
- MARS2 p.Gln123Pro Uncertain significance Single submitter
- MARS2 p.Glu249Val Uncertain significance Single submitter
- MARS2 p.Glu445Gln Uncertain significance Single submitter
- MARS2 p.Gly270Arg Uncertain significance Single submitter
- MARS2 p.His340Gln Uncertain significance Single submitter
- MARS2 p.Ile469Thr Uncertain significance Single submitter
- MARS2 p.Leu514Phe Uncertain significance Single submitter
- MARS2 p.Leu531Pro Uncertain significance Single submitter
- MARS2 p.Phe125Leu Uncertain significance Single submitter
- MARS2 p.Phe467Leu Uncertain significance Single submitter
- MARS2 p.Pro202Ser Uncertain significance Single submitter
- MARS2 p.Pro251Leu Uncertain significance Single submitter
- MARS2 p.Thr366Ala Uncertain significance Single submitter
- MARS2 p.Tyr464Cys Uncertain significance Single submitter
- MARS2 p.Val355Ala Uncertain significance Single submitter
All MARS2 variants
Pathogenic 2
- p.Cys344fs Single submitter combined oxidative phosphorylation defect type 25
- p.Gly228fs No assertion criteria spastic ataxia 3
Likely pathogenic 3
- p.Gln184Ter Single submitter MARS2-related disorders
- p.Thr49Ala Single submitter
- p.Trp279Ter Single submitter spastic ataxia 3
Conflicting classifications of pathogenicity 11
- p.Ala15Thr Single submitter ⚠
- p.Arg546= Single submitter
- p.Gly291fs Single submitter
- p.His59Tyr Single submitter ⚠
- p.Ile233Val Single submitter ⚠
- p.Leu21Val Single submitter spastic ataxia 3 ⚠
- p.Leu374= Single submitter
- p.Leu449= Single submitter
- p.Phe467= Single submitter
- p.Ser19= Single submitter
- p.Thr83Ala Single submitter ⚠
Benign 6
- p.Arg157= Multiple submitters
- p.Arg8= Multiple submitters
- p.Pro202= Multiple submitters
- p.Thr4= Multiple submitters
- p.Val527Ala Multiple submitters spastic ataxia 3
- c.*166_*168dup Single submitter
Benign/Likely benign 8
- p.Asp42Asn Multiple submitters
- p.Leu20= Multiple submitters
- p.Leu250= Multiple submitters
- p.Leu539= Multiple submitters
- p.Phe429= Multiple submitters
- p.Pro267= Multiple submitters
- p.Pro433Ala Multiple submitters
- p.Val391= Multiple submitters
Likely benign 55
- p.Asn466= Multiple submitters
- p.His240= Multiple submitters
- p.Leu403= Multiple submitters
- p.Leu587= Multiple submitters
- p.Thr183= Multiple submitters
- p.Tyr29= Multiple submitters
- p.Tyr470= Multiple submitters
- p.Val241Ile Multiple submitters
- c.*248_*261del Single submitter
- c.*7A>G Single submitter
- p.Ala452= Single submitter
- p.Arg12= Single submitter
- p.Arg137= Single submitter
- p.Arg468Leu Single submitter
- p.Arg77= Single submitter
- p.Asn229Asp Single submitter
- p.Asn496= Single submitter
- p.Asp311= Single submitter
- p.Cys170= Single submitter
- p.Gln525= Single submitter
- p.Glu398= Single submitter
- p.Gly163= Single submitter
- p.Gly25= Single submitter
- p.Gly265= Single submitter
- p.Gly434= Single submitter
- p.Gly437= Single submitter
- p.His149= Single submitter
- p.His261= Single submitter
- p.His306= Single submitter
- p.Ile335= Single submitter
- p.Leu18= Single submitter
- p.Leu253= Single submitter
- p.Leu287= Single submitter
- p.Leu72= Single submitter
- p.Lys350= Single submitter
- p.Lys534= Single submitter
- p.Phe52= Single submitter
- p.Pro251= Single submitter
- p.Pro331= Single submitter
- p.Pro358= Single submitter
- p.Pro422= Single submitter
- p.Pro529= Single submitter
- p.Ser216= Single submitter
- p.Ser254= Single submitter
- p.Ser273= Single submitter
- p.Ser31= Single submitter
- p.Thr139= Single submitter
- p.Thr366= Single submitter
- p.Thr49= Single submitter
- p.Tyr292= Single submitter
- p.Val153Met Single submitter
- p.Val241= Single submitter
- p.Val480= Single submitter
- p.Ala461= No assertion criteria MARS2-related disorder
- p.Ser16= No assertion criteria MARS2-related disorder
Uncertain significance 192
- p.Ala141Val Multiple submitters ⚠
- p.Ala402Ser Multiple submitters ⚠
- p.Arg137Leu Multiple submitters
- p.Arg142Trp Multiple submitters combined oxidative phosphorylation defect type 25 ⚠
- p.Arg224Gln Multiple submitters ⚠
- p.Arg409Gly Multiple submitters ⚠
- p.Arg414Ile Multiple submitters spastic ataxia 3 ⚠
- p.Arg568Gly Multiple submitters ⚠
- p.Arg592Gln Multiple submitters ⚠
- p.Arg79Gln Multiple submitters ⚠
- p.Asn285Ser Multiple submitters ⚠
- p.Cys175Ser Multiple submitters ⚠
- p.Glu249Lys Multiple submitters ⚠
- p.Gly574Val Multiple submitters ⚠
- p.His338Arg Multiple submitters ⚠
- p.Ile136Met Multiple submitters ⚠
- p.Ile266Thr Multiple submitters ⚠
- p.Ile276Leu Multiple submitters ⚠
- p.Leu159Val Multiple submitters ⚠
- p.Leu243Phe Multiple submitters ⚠
- p.Leu2Arg Multiple submitters ⚠
- p.Leu406Val Multiple submitters
- p.Pro230Arg Multiple submitters ⚠
- p.Pro230Leu Multiple submitters ⚠
- p.Pro321Leu Multiple submitters ⚠
- p.Ser156Phe Multiple submitters ⚠
- p.Ser30Cys Multiple submitters ⚠
- p.Ser418Phe Multiple submitters ⚠
- p.Thr139Met Multiple submitters
- p.Thr13Met Multiple submitters ⚠
- p.Thr183Ile Multiple submitters ⚠
- p.Trp151Leu Multiple submitters ⚠
- p.Trp151Ser Multiple submitters ⚠
- p.Tyr165Cys Multiple submitters combined oxidative phosphorylation defect type 25 ⚠
- p.Tyr29Phe Multiple submitters ⚠
- p.Tyr365Cys Multiple submitters ⚠
- p.Tyr558Cys Multiple submitters ⚠
- p.Val255Met Multiple submitters ⚠
- p.Ala128Ser Single submitter ⚠
- p.Ala146Gly Single submitter ⚠
- p.Ala180Val Single submitter ⚠
- p.Ala282Ser Single submitter ⚠
- p.Ala282Val Single submitter ⚠
- p.Ala295Thr Single submitter ⚠
- p.Ala36Ser Single submitter ⚠
- p.Ala40_Asp42del Single submitter
- p.Ala402fs Single submitter
- p.Ala423Gly Single submitter ⚠
- p.Ala442= Single submitter
- p.Ala503Ser Single submitter ⚠
- p.Ala532Gly Single submitter spastic ataxia 3 ⚠
- p.Ala590Thr Single submitter ⚠
- p.Arg137Cys Single submitter
- p.Arg142Gln Single submitter ⚠
- p.Arg144Leu Single submitter ⚠
- p.Arg227Gln Single submitter ⚠
- p.Arg257Pro Single submitter ⚠
- p.Arg334His Single submitter ⚠
- p.Arg364Cys Single submitter ⚠
- p.Arg364His Single submitter ⚠
- p.Arg364Leu Single submitter ⚠
- p.Arg371Cys Single submitter ⚠
- p.Arg376Trp Single submitter
- p.Arg409Gln Single submitter ⚠
- p.Arg414Ter Single submitter
- p.Arg567Trp Single submitter
- p.Arg584Gly Single submitter ⚠
- p.Arg592Gly Single submitter ⚠
- p.Arg77Cys Single submitter ⚠
- p.Arg8Leu Single submitter ⚠
- p.Arg8Ser Single submitter ⚠
- p.Asn229= Single submitter
- p.Asn229Lys Single submitter ⚠
- p.Asn229Thr Single submitter ⚠
- p.Asn285= Single submitter
- p.Asn285Ile Single submitter ⚠
- p.Asn416Thr Single submitter ⚠
- p.Asp247Gly Single submitter ⚠
- p.Asp252Asn Single submitter
- p.Asp272Gly Single submitter ⚠
- p.Asp385His Single submitter ⚠
- p.Asp388Glu Single submitter ⚠
- p.Asp39Asn Single submitter ⚠
- p.Asp462Glu Single submitter ⚠
- p.Asp465Gly Single submitter ⚠
- p.Cys344Gly Single submitter ⚠
- p.Cys361Ser Single submitter ⚠
- p.Cys384Tyr Single submitter ⚠
- p.Cys479Tyr Single submitter ⚠
- p.Cys562Phe Single submitter ⚠
- p.Cys73Phe Single submitter ⚠
- p.Gln123Leu Single submitter ⚠
- p.Gln123Pro Single submitter ⚠
- p.Gln126Ter Single submitter
- p.Gln482His Single submitter ⚠
- p.Glu249Val Single submitter ⚠
- p.Glu296Asp Single submitter ⚠
- p.Glu398Gln Single submitter ⚠
- p.Glu432Gly Single submitter ⚠
- p.Glu445Gln Single submitter ⚠
- p.Glu550Lys Single submitter ⚠
- p.Gly11Glu Single submitter ⚠
- p.Gly167Ser Single submitter ⚠
- p.Gly270Arg Single submitter ⚠
- p.Gly270Glu Single submitter ⚠
- p.Gly291Asp Single submitter ⚠
- p.Gly574fs Single submitter
- p.His239Leu Single submitter ⚠
- p.His316Leu Single submitter ⚠
- p.His316Tyr Single submitter ⚠
- p.His340Gln Single submitter ⚠
- p.His463Tyr Single submitter ⚠
- p.His560Tyr Single submitter ⚠
- p.Ile130Thr Single submitter ⚠
- p.Ile136Asn Single submitter ⚠
- p.Ile136Ser Single submitter ⚠
- p.Ile415Leu Single submitter ⚠
- p.Ile469Thr Single submitter ⚠
- p.Ile51Val Single submitter ⚠
- p.Leu154= Single submitter
- p.Leu197His Single submitter spastic ataxia 3 ⚠
- p.Leu20Pro Single submitter ⚠
- p.Leu253Met Single submitter
- p.Leu283fs Single submitter
- p.Leu283Val Single submitter ⚠
- p.Leu406Ile Single submitter
- p.Leu449_Ala452del Single submitter
- p.Leu514Phe Single submitter ⚠
- p.Leu523Phe Single submitter
- p.Leu524Pro Single submitter ⚠
- p.Leu531Pro Single submitter ⚠
- p.Leu78= Single submitter
- p.Leu7Phe Single submitter ⚠
- p.Leu9Gln Single submitter ⚠
- p.Lys310Glu Single submitter combined oxidative phosphorylation defect type 25 ⚠
- p.Lys310Met Single submitter ⚠
- p.Lys347Arg Single submitter ⚠
- p.Lys347Glu Single submitter ⚠
- p.Lys458Asn Single submitter ⚠
- p.Met1Ile Single submitter
- p.Met329Thr Single submitter ⚠
- p.Phe125Leu Single submitter ⚠
- p.Phe467Leu Single submitter ⚠
- p.Phe520= Single submitter
- p.Pro202Ser Single submitter ⚠
- p.Pro251Leu Single submitter ⚠
- p.Pro267Ser Single submitter spastic ataxia 3 ⚠
- p.Pro302Ala Single submitter ⚠
- p.Pro302Ser Single submitter ⚠
- p.Pro358Arg Single submitter ⚠
- p.Pro380Ser Single submitter ⚠
- p.Pro529Leu Single submitter ⚠
- p.Pro571Ser Single submitter ⚠
- p.Pro81Thr Single submitter ⚠
- p.Ser260Gly Single submitter ⚠
- p.Ser30Thr Single submitter ⚠
- p.Ser349Cys Single submitter ⚠
- p.Ser351Arg Single submitter ⚠
- p.Ser431Arg Single submitter ⚠
- p.Ser439Ter Single submitter
- p.Ser478Thr Single submitter ⚠
- p.Ser542Cys Single submitter ⚠
- p.Thr234Asn Single submitter ⚠
- p.Thr288Ser Single submitter ⚠
- p.Thr360Ala Single submitter ⚠
- p.Thr366Ala Single submitter ⚠
- p.Thr528Ile Single submitter ⚠
- p.Thr585Ser Single submitter ⚠
- p.Thr593Ala Single submitter ⚠
- p.Thr593Ile Single submitter ⚠
- p.Trp151Cys Single submitter ⚠
- p.Trp151Ter Single submitter
- p.Trp168Ter Single submitter spastic ataxia 3
- p.Trp264Ter Single submitter
- p.Trp301Ter Single submitter
- p.Tyr28= Single submitter
- p.Tyr28His Single submitter ⚠
- p.Tyr365His Single submitter
- p.Tyr386Cys Single submitter
- p.Tyr387Asn Single submitter ⚠
- p.Tyr387Cys Single submitter ⚠
- p.Tyr447Cys Single submitter ⚠
- p.Tyr464Cys Single submitter ⚠
- p.Val182= Single submitter
- p.Val195Ala Single submitter ⚠
- p.Val255Leu Single submitter
- p.Val355Ala Single submitter ⚠
- p.Val453Leu Single submitter ⚠
- p.Val453Met Single submitter ⚠
- p.Val487Ile Single submitter ⚠
- p.Val512Met Single submitter ⚠
- p.Ala461Gly No assertion criteria ⚠