Is RTN2 p.Arg425Gln (c.1274G>A) pathogenic?

RTN2 p.Arg425Gln (c.1274G>A) is classified Benign/Likely benign in RTN2 (ClinVar, Multiple submitters, 4 submitters; last evaluated 2025-12-09). AlphaMissense: likely benign (0.0788); Rare (popmax 0.601%, afr); Associated with Spastic paraplegia.

What condition is associated with RTN2 p.Arg425Gln (c.1274G>A)?

RTN2 p.Arg425Gln (c.1274G>A) is associated with Spastic paraplegia (ClinVar).

How common is RTN2 p.Arg425Gln (c.1274G>A) in the general population?

RTN2 p.Arg425Gln (c.1274G>A) is rare (popmax 0.601%, afr) (gnomAD).

What do computational predictors say about RTN2 p.Arg425Gln (c.1274G>A)?

AlphaMissense predicts likely benign (score 0.0788) — an in-silico prediction, not a clinical call.

RTN2 p.Arg425Gln

c.1274G>A · rs35461805 · VCV448171

Benign/Likely benign Multiple submitters

Classification

Benign/Likely benign

ClinVar

Review

Multiple submitters ★★☆☆

ClinVar · 4 submitters

AlphaMissense

likely benign

0.0788 · ACMG-weighted

gnomAD v4.1

Rare

rare (popmax 0.601%, afr)

Missense predictors

0/3 damaging

AlphaMissense, REVEL, SaProt

Conditions

Spastic paraplegia

Computational evidence

Protein change
AlphaMissense	0.0788	likely benign
REVEL	0.04	moderately benign-leaning
SaProt	LLR -4.94	tolerated
⇒ AlphaMissense, REVEL and SaProt all call this tolerated.
Splicing
SpliceAI	—	not assessed · no allele here is in the release
Constraint at this position
Conservation	phyloP -3.3	GERP -3.1
Regional constraint	o/e 0.95	unconstrained · MANE · 11 kb · p 0.23 · regional, not allelic
Overall
CADD	12.42	PHRED · no single calibrated threshold — expert panels disagree

AlphaMissense carries the ACMG weight for missense; REVEL and SaProt are independent agreement signals.

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 0.601% African / African-American · 451/75,000 alleles

Filtering AF 0.555% ClinGen BA1/BS1 input FAF95, joint · FAF99 0.537%

Overall 0.0346% 557/1,611,644 alleles · rare exomes 305/1,459,398 · genomes 252/152,246 · joint denominator

Ancestry group	Frequency	1 in
African / African-Americangrpmax	0.601%	166
Admixed American	0.0569%	1,756
Remaining individualsnot grpmax-eligible	0.024%	4,160
European (non-Finnish)	0.00466%	21,438
Finnishnot grpmax-eligible	0.00157%	63,754
South Asian	0.00111%	90,450

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: RTN2
Protein: p.Arg425Gln
Coding: c.1274G>A
MANE Select: NM_005619
dbSNP: rs35461805
Type: single nucleotide variant
Location: chr19:45488954 (GRCh38)
Gene constraint: LOEUF 0.748 · pLI <0.01 (gnomAD)
Structure: AlphaFold pLDDT 50.99
Residue 425: a UniProt-annotated variant site (in hmnr11; likely pathogenic); a UniProt-annotated variant site (in spg12; likely pathogenic); a UniProt-annotated variant site (in); the reticulon domain

Full RTN2 gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Disease context

Spastic paraplegia — disease report on Sugi Atlas → Associated genes, phenotypes, clinical trials & treatments

Similar variants

RTN2 p.Arg425Trp Likely benign ★ ⚠ same residue
RTN2 p.Arg425Pro Uncertain significance ★ ⚠ same residue
RTN2 p.Glu50Ter Pathogenic/Likely pathogenic ★★ same condition
RTN2 c.1497+3G>A Uncertain significance ★★ same condition
RTN2 p.Ala529Thr Uncertain significance ★★ ⚠ same condition
RTN2 p.Arg152Pro Uncertain significance ★★ ⚠ same condition
RTN2 p.Arg252Trp Uncertain significance ★★ ⚠ same condition
RTN2 p.Arg398Cys Uncertain significance ★★ ⚠ same condition

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/rtn2-p-arg425gln (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown