Is SPAST p.Pro105= (c.315G>A) pathogenic?

SPAST p.Pro105= (c.315G>A) is classified Likely benign in SPAST (ClinVar, Single submitter, 1 submitter; last evaluated 2025-11-05). Ultra-rare (popmax 0.015%, afr); Associated with hereditary spastic paraplegia 4.

What condition is associated with SPAST p.Pro105= (c.315G>A)?

SPAST p.Pro105= (c.315G>A) is associated with hereditary spastic paraplegia 4 (ClinVar).

How common is SPAST p.Pro105= (c.315G>A) in the general population?

SPAST p.Pro105= (c.315G>A) is ultra-rare (popmax 0.015%, afr) (gnomAD).

How is Autosomal dominant spastic paraplegia type 4 inherited?

Typically Autosomal dominant (Orphanet). A genetic counselor can assess individual risk for a family.

SPAST p.Pro105=

c.315G>A · rs761254257 · VCV1366529

Likely benign Single submitter

Classification

Likely benign

ClinVar

Review

Single submitter ★☆☆☆

ClinVar · 1 submitter

gnomAD v4.1

Rare

ultra-rare (popmax 0.015%, afr)

Conditions

hereditary spastic paraplegia 4

Computational evidence

Protein change
Missense predictors do not apply to this variant type; conservation carries the signal.
Splicing
SpliceAI	—	not assessed · no allele here is in the release
Constraint at this position
Conservation	phyloP -0.2	GERP -4.2, phastCons 0.001
Overall
CADD	8.752	PHRED · no single calibrated threshold — expert panels disagree

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 0.015% African / African-American · 11/73,550 alleles

Filtering AF 0.0083% ClinGen BA1/BS1 input FAF95, joint · FAF99 0.00644%

Overall 0.000832% 13/1,562,236 alleles · ultra-rare exomes 7/1,410,380 · genomes 6/151,856 · joint denominator

Ancestry group	Frequency	1 in
African / African-Americangrpmax	0.015%	6,686
European (non-Finnish)	0.000173%	577,101

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: SPAST
Protein: p.Pro105=
Coding: c.315G>A
MANE Select: NM_014946
dbSNP: rs761254257
Type: single nucleotide variant
Location: chr2:32064146 (GRCh38)
Gene constraint: LOEUF 0.69 · pLI 0.11 · ClinGen HI 3/3 (gnomAD)
Structure: AlphaFold pLDDT 77.03 · 7 PDB
Residue 105: a UniProt-annotated variant site (in spg4); the 'required for interaction with rtn1' region; the 'required for midbody localization' region

Full SPAST gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Disease context

hereditary spastic paraplegia 4 — disease report on Sugi Atlas → Associated genes, phenotypes, clinical trials & treatments

For Autosomal dominant spastic paraplegia type 4 (Orphanet):

Inheritance: Autosomal dominant
Onset: Adolescent, Adult, Childhood, Infancy
Prevalence: 1-9 / 1 000 000 (Portugal)

Similar variants

SPAST p.Pro105fs Likely pathogenic ★ same residue
SPAST p.Pro105Arg Uncertain significance ★ ⚠ same residue
SPAST c.1004+1G>C Pathogenic/Likely pathogenic ★★ same condition
SPAST c.1004+1G>T Pathogenic ★★ same condition
SPAST c.1004+2T>G Pathogenic ★★ same condition
SPAST c.1098+1G>A Pathogenic ★★ same condition
SPAST c.1099-1G>A Pathogenic ★★ same condition
SPAST c.1099-2A>C Pathogenic/Likely pathogenic ★★ same condition

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/spast-p-pro105 (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown