Is TP53 p.Cys277Phe (c.830G>T) pathogenic?

TP53 p.Cys277Phe (c.830G>T) is classified Conflicting classifications of pathogenicity in TP53 (ClinVar, Conflicting submitters, 7 submitters; last evaluated 2025-03-21). AlphaMissense: likely pathogenic (0.9904); Very rare (popmax 8.47e-05%, nfe); Associated with TP53-related disorder.

What condition is associated with TP53 p.Cys277Phe (c.830G>T)?

TP53 p.Cys277Phe (c.830G>T) is associated with TP53-related disorder, hereditary neoplastic syndrome, Li-Fraumeni syndrome (ClinVar).

How common is TP53 p.Cys277Phe (c.830G>T) in the general population?

TP53 p.Cys277Phe (c.830G>T) is very rare (popmax 8.47e-05%, nfe) (gnomAD).

What do computational predictors say about TP53 p.Cys277Phe (c.830G>T)?

AlphaMissense predicts likely pathogenic (score 0.9904) — an in-silico prediction, not a clinical call.

TP53 p.Cys277Phe

c.830G>T · rs763098116 · VCV458567

Conflicting classifications of pathogenicity Conflicting submitters

Classification

Conflicting classifications of pathogenicity

ClinVar

Review

Conflicting submitters ★☆☆☆

ClinVar · 7 submitters

AlphaMissense

likely pathogenic

0.9904 · ACMG-weighted

gnomAD v4.1

Rare

very rare (popmax 8.47e-05%, nfe)

Missense predictors

3/3 damaging

AlphaMissense, REVEL, SaProt

Conditions

TP53-related disorder

Computational evidence

Protein change
AlphaMissense	0.9904	likely pathogenic · top 9% in TP53
REVEL	0.932	strongly pathogenic-leaning
SaProt	LLR -11.97	damaging
⇒ AlphaMissense, REVEL and SaProt all call this damaging.
Splicing
SpliceAI	—	not assessed · no allele here is in the release
Constraint at this position
Conservation	phyloP 7.7	GERP 2.5, phastCons 1
Regional constraint	o/e 0.89	unconstrained · MANE · 6,982 bp · p 0.0085 · regional, not allelic
Overall
CADD	27.9	PHRED · no single calibrated threshold — expert panels disagree

AlphaMissense carries the ACMG weight for missense; REVEL and SaProt are independent agreement signals.

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 8.47e-05% European (non-Finnish) · 1/1,180,016 alleles

Overall 6.2e-05% 1/1,614,092 alleles · very rare exomes 1/1,461,856 · genomes 0/152,236 · joint denominator

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: TP53
Protein: p.Cys277Phe
Coding: c.830G>T
MANE Select: NM_000546
dbSNP: rs763098116
Type: single nucleotide variant
Location: chr17:7673790 (GRCh38)
Gene constraint: LOEUF 0.449 · pLI 1.00 · ClinGen HI 3/3 (gnomAD)
Structure: AlphaFold pLDDT 75.83 · 321 PDB
Residue 277: a DNA-binding region; the 'interaction with axin1' region; a UniProt-annotated variant site (in sporadic cancers; somatic mutation); a UniProt-annotated variant site (in an osteosarcoma with no family history; germline mutation and in sporadic cancers; somatic mutation); the 'interaction with e4f1' region; the 'interaction with dna' region; the 'interaction with ccar2' region; the 'interaction with hipk1' region; the 'required for interaction with znf385a' region

Full TP53 gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Disease context

TP53-related disorder

Also linked: hereditary neoplastic syndrome Li-Fraumeni syndrome

Similar variants

TP53 p.Cys277Gly Likely pathogenic ★★★ same residue
TP53 p.Cys277Arg Uncertain significance ★★★ ⚠ same residue
TP53 p.Cys277Ter Pathogenic ★★ same residue
TP53 p.Cys277Ser Uncertain significance ★★ ⚠ same residue
TP53 p.Cys277Trp Uncertain significance ★★ ⚠ same residue
TP53 p.Cys277= Benign/Likely benign ★★ same residue
TP53 p.Cys277Tyr Conflicting classifications of pathogenicity ★ ⚠ same residue
TP53 p.Cys277fs Pathogenic ★ same residue

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/tp53-p-cys277phe (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown