Is CD276 p.Val134Ile (c.400G>A) pathogenic?

CD276 p.Val134Ile (c.400G>A) is classified Uncertain significance in CD276 (ClinVar, Single submitter, 1 submitter; last evaluated 2023-11-03). AlphaMissense: likely benign (0.0872); Ultra-rare (popmax 0.0451%, amr).

How common is CD276 p.Val134Ile (c.400G>A) in the general population?

CD276 p.Val134Ile (c.400G>A) is ultra-rare (popmax 0.0451%, amr) (gnomAD).

What do computational predictors say about CD276 p.Val134Ile (c.400G>A)?

AlphaMissense predicts likely benign (score 0.0872) — an in-silico prediction, not a clinical call.

CD276 p.Val134Ile

c.400G>A · rs538781194 · VCV3140486

Uncertain significance Single submitter

Classification

Uncertain significance

ClinVar

Review

Single submitter ★☆☆☆

ClinVar · 1 submitter

AlphaMissense

likely benign

0.0872 · ACMG-weighted

gnomAD v4.1

Rare

ultra-rare (popmax 0.0451%, amr)

Missense predictors

1/3 damaging

AlphaMissense, REVEL, SaProt

Computational evidence

Protein change
AlphaMissense	0.0872	likely benign
REVEL	0.078	moderately benign-leaning
SaProt	LLR -12.60	damaging
⇒ SaProt calls this damaging; AlphaMissense and REVEL do not.
Splicing
SpliceAI	—	not assessed · no allele here is in the release
Constraint at this position
Conservation	phyloP 7.2	GERP 1.4, phastCons 1
Regional constraint	o/e 0.88	unconstrained · MANE · 13 kb · p 0.0018 · regional, not allelic
Overall
CADD	23.5	PHRED · no single calibrated threshold — expert panels disagree

AlphaMissense carries the ACMG weight for missense; REVEL and SaProt are independent agreement signals.

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 0.0451% Admixed American · 27/59,922 alleles

Filtering AF 0.0317% ClinGen BA1/BS1 input FAF95, joint · FAF99 0.0274%

Overall 0.00647% 104/1,608,386 alleles · ultra-rare exomes 82/1,456,070 · genomes 22/152,316 · joint denominator

Ancestry group	Frequency	1 in
Admixed Americangrpmax	0.0451%	2,219
Remaining individualsnot grpmax-eligible	0.0257%	3,896
East Asian	0.00892%	11,209
Ashkenazi Jewishnot grpmax-eligible	0.00679%	14,720
African / African-American	0.00533%	18,753
European (non-Finnish)	0.00399%	25,075
South Asian	0.0033%	30,283
Finnishnot grpmax-eligible	0.00165%	60,550

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: CD276
Protein: p.Val134Ile
Coding: c.400G>A
MANE Select: NM_001024736
dbSNP: rs538781194
Type: single nucleotide variant
Location: chr15:73702575 (GRCh38)
Gene constraint: LOEUF 0.888 · pLI <0.01 (gnomAD)
Structure: AlphaFold pLDDT 83.63 · 3 PDB
Residue 134: the ig-like v-type 1 domain

Full CD276 gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Similar variants

CD276 p.Val134Ala Uncertain significance ★ ⚠ same residue
CD276 p.Leu419= Benign ★★ same type
CD276 p.Ala132Ser Uncertain significance ★ ⚠ same type
CD276 p.Ala139Ser Uncertain significance ★ ⚠ same type
CD276 p.Ala28Ser Uncertain significance ★ ⚠ same type
CD276 p.Ala312Thr Uncertain significance ★ ⚠ same type
CD276 p.Ala350Thr Uncertain significance ★ ⚠ same type
CD276 p.Ala351Val Uncertain significance ★ ⚠ same type

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/cd276-p-val134ile (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown