Is DACT3 p.Arg286Cys (c.856C>T) pathogenic?

DACT3 p.Arg286Cys (c.856C>T) is classified Uncertain significance in DACT3 (ClinVar, Single submitter, 1 submitter; last evaluated 2024-06-07). AlphaMissense: likely benign (0.2499); Rare (popmax 0.163%, mid).

How common is DACT3 p.Arg286Cys (c.856C>T) in the general population?

DACT3 p.Arg286Cys (c.856C>T) is rare (popmax 0.163%, mid) (gnomAD).

What do computational predictors say about DACT3 p.Arg286Cys (c.856C>T)?

AlphaMissense predicts likely benign (score 0.2499) — an in-silico prediction, not a clinical call.

DACT3 p.Arg286Cys

c.856C>T · rs528739876 · VCV2349387

Uncertain significance Single submitter

Classification

Uncertain significance

ClinVar

Review

Single submitter ★☆☆☆

ClinVar · 1 submitter

AlphaMissense

likely benign

0.2499 · ACMG-weighted

gnomAD v4.1

Rare

rare (popmax 0.163%, mid)

Missense predictors

0/3 damaging

AlphaMissense, REVEL, SaProt

Computational evidence

Protein change
AlphaMissense	0.2499	likely benign
REVEL	0.196	benign-leaning
SaProt	LLR -8.61	tolerated
⇒ AlphaMissense, REVEL and SaProt all call this tolerated.
Splicing
SpliceAI	—	not assessed · no allele here is in the release
Constraint at this position
Conservation	phyloP 6	GERP 0.74, phastCons 1
Regional constraint	o/e 1.09	unconstrained · MANE · 1,201 bp · p 0.027 · regional, not allelic
Overall
CADD	22.7	PHRED · no single calibrated threshold — expert panels disagree

ClinVar classifies this uncertain; the computational predictors unanimously lean tolerated — a triage signal, not a reclassification

AlphaMissense carries the ACMG weight for missense; REVEL and SaProt are independent agreement signals.

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 0.163% Middle Eastern · 4/2,454 alleles

Filtering AF 0.0556% ClinGen BA1/BS1 input FAF95, joint · FAF99 0.0335%

Overall 0.0244% 265/1,085,222 alleles · rare

Ancestry group	Frequency	1 in
Middle Easterngrpmax	0.163%	614
Ashkenazi Jewishnot grpmax-eligible	0.0612%	1,634
Admixed American	0.0424%	2,360
Remaining individualsnot grpmax-eligible	0.0394%	2,535
European (non-Finnish)	0.0257%	3,898
South Asian	0.00429%	23,304
African / African-American	0.00169%	59,036

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: DACT3
Protein: p.Arg286Cys
Coding: c.856C>T
MANE Select: NM_145056
dbSNP: rs528739876
Type: single nucleotide variant
Location: chr19:46649516 (GRCh38)
Gene constraint: LOEUF 0.703 · pLI 0.65 (gnomAD)
Structure: AlphaFold pLDDT 53.51
Residue 286: the 'disordered' region

Full DACT3 gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Similar variants

DACT3 p.Ala179Pro Uncertain significance ★ ⚠ same type
DACT3 p.Ala181Ser Uncertain significance ★ ⚠ same type
DACT3 p.Ala181Thr Uncertain significance ★ ⚠ same type
DACT3 p.Ala194Thr Uncertain significance ★ ⚠ same type
DACT3 p.Ala299Gly Uncertain significance ★ ⚠ same type
DACT3 p.Ala318Ser Uncertain significance ★ ⚠ same type
DACT3 p.Ala338Pro Uncertain significance ★ ⚠ same type
DACT3 p.Ala363Val Uncertain significance ★ ⚠ same type

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/dact3-p-arg286cys (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown