Is WT1 p.Ile353= (c.1059C>T) pathogenic?

WT1 p.Ile353= (c.1059C>T) is classified Conflicting classifications of pathogenicity in WT1 (ClinVar, Conflicting submitters, 5 submitters; last evaluated 2025-11-19). Ultra-rare (popmax 0.0217%, amr); Associated with Wilms tumor 1.

What condition is associated with WT1 p.Ile353= (c.1059C>T)?

WT1 p.Ile353= (c.1059C>T) is associated with Wilms tumor 1, Frasier syndrome, WAGR syndrome (ClinVar).

How common is WT1 p.Ile353= (c.1059C>T) in the general population?

WT1 p.Ile353= (c.1059C>T) is ultra-rare (popmax 0.0217%, amr) (gnomAD).

WT1 p.Ile353=

c.1059C>T · rs527655625 · VCV290725

Conflicting classifications of pathogenicity Conflicting submitters

Classification

Conflicting classifications of pathogenicity

ClinVar

Review

Conflicting submitters ★☆☆☆

ClinVar · 5 submitters

gnomAD v4.1

Rare

ultra-rare (popmax 0.0217%, amr)

Conditions

Wilms tumor 1

Computational evidence

Protein change
Missense predictors do not apply to this variant type; conservation carries the signal.
REVEL	0.088	moderately benign-leaning
Splicing
SpliceAI	—	not assessed · no allele here is in the release
Constraint at this position
Conservation	phyloP 1.8	GERP 0.61, phastCons 1
Overall
CADD	6.399	PHRED · no single calibrated threshold — expert panels disagree

Population frequency

gnomAD v4.1 · exomes + genomes

Grpmax group 0.0217% Admixed American · 13/60,034 alleles

Filtering AF 0.0127% ClinGen BA1/BS1 input FAF95, joint · FAF99 0.0101%

Overall 0.00558% 90/1,614,248 alleles · ultra-rare exomes 82/1,461,888 · genomes 8/152,360 · joint denominator

Ancestry group	Frequency	1 in
Admixed Americangrpmax	0.0217%	4,618
Remaining individualsnot grpmax-eligible	0.0096%	10,418
European (non-Finnish)	0.00585%	17,102
African / African-American	0.00266%	37,532

Rarity supports pathogenicity (ACMG PM2); frequency above a disease-specific BA1/BS1 threshold argues against it. A variant frequent in one ancestry group can be near-absent in others, so a single global figure can mislead.

Variant & gene context

Gene: WT1
Protein: p.Ile353=
Coding: c.1059C>T
MANE Select: NM_024426
dbSNP: rs527655625
Type: single nucleotide variant
Location: chr11:32400002 (GRCh38)
Gene constraint: LOEUF 0.253 · pLI 1.00 · ClinGen HI 3/3 (gnomAD)
Structure: AlphaFold pLDDT 52.09 · 31 PDB
Residue 353: a β-strand

Full WT1 gene report on Sugi Atlas → Gene function, pathways, protein structure, interactions & more

Disease context

Wilms tumor 1 — disease report on Sugi Atlas → Associated genes, phenotypes, clinical trials & treatments

Also linked: Frasier syndrome WAGR syndrome Denys-Drash syndrome

Similar variants

WT1 p.Ile353Asn Uncertain significance ★★ same residue
WT1 p.Ile353Val Uncertain significance ★★ same residue
WT1 c.1354+2T>C Pathogenic/Likely pathogenic ★★ same condition
WT1 c.1447+2T>C Pathogenic ★★ same condition
WT1 c.1447+4C>T Pathogenic/Likely pathogenic ★★ same condition
WT1 c.1447+5G>A Pathogenic/Likely pathogenic ★★ same condition
WT1 c.1448-1G>A Pathogenic/Likely pathogenic ★★ same condition
WT1 p.Arg374Ter Pathogenic ★★ same condition

Built on 18 open, versioned datasets — ClinVar, gnomAD, AlphaMissense, REVEL, ClinGen & more — plus Sugi Variant's own cross-source analysis. Source data 2026-01–2026-10, ClinVar 2026-10. Full sources & versions →

Cite Sugi Variant

Gür T. Sugi Variant: A Deterministic Per-Variant Germline Reference from a Knowledge Graph for Humans and AI Agents. 2026. doi:10.5281/zenodo.21509445. This variant: https://sugi.bio/variant/wt1-p-ile353 (accessed [today's date]).

Preprint & method: sugi.bio/variant/method Machine-readable: this page as Markdown